Abstract:
:The M54 transgenic mouse line, which carries the 17.2.25 Ig mu heavy chain gene, rearranges Ig heavy chains and expresses both transgenic and endogenous mu. B cell lineage development is selectively impaired in these mice and cells that simultaneously express transgenic and endogenous mu ('double-producers') are common amongst the B cells and plasma cells that do develop. Weaver, Imanishi-kari, Baltimore and colleagues failed to obtain double-producing hybridomas from M54 mice; however, molecular and serologic studies presented here show that such hybridomas are readily generated. These hybridomas are extremely unstable and rapidly yield variants producing either transgenic or endogenous mu. Therefore the stable cloned lines we obtained, like Weaver et al., were almost all single or non-producers. We also found that the VH gene usage in our hybridomas was skewed towards the JH proximal (VHQ52, VH81X) families, supporting the idea that the expression of the M54 transgene alters the endogenous Ig repertoire.
journal_name
Int Immunoljournal_title
International immunologyauthors
Lam KP,Herzenberg LA,Stall AMdoi
10.1093/intimm/5.9.1011subject
Has Abstractpub_date
1993-09-01 00:00:00pages
1011-22issue
9eissn
0953-8178issn
1460-2377journal_volume
5pub_type
杂志文章abstract::It is now generally recognized that bone marrow is the survival niche for antigen-specific plasma cells with long-term immunological memory. These cells release antibodies into the circulation, needed to prime effector cells in the secondary immune response. These antibodies participate in the surveillance for antigen...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy048
更新日期:2018-08-30 00:00:00
abstract::In view of our recent report that T cell receptor (TCR) alpha chain restriction fragment length polymorphism (RFLP) is associated with multiple sclerosis (MS), we have assessed the possibility that HLA-DR2, HLA-DPw4, and TCR alpha chain RFLPs may interact to increase the risk of developing MS. Detection of TCR alpha c...
journal_title:International immunology
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doi:10.1093/intimm/4.2.177
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pub_type: 杂志文章
doi:10.1093/intimm/12.11.1511
更新日期:2000-11-01 00:00:00
abstract::Transforming growth factor (TGF)-beta has been shown to stimulate isotype switching to IgA in cultures of lipopolysaccharide (LPS)-stimulated B cells. The induction of isotype switching is associated with the appearance of novel germline alpha transcripts that cannot be found in cultures stimulated with LPS alone. TGF...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.1.113
更新日期:1994-01-01 00:00:00
abstract::CD4 T cells are not thought to play a significant role in generating an effective primary CD8 T cell response to most viral infections. We have challenged this view by demonstrating that antigen-specific CD4 T cells can indeed suppress the proliferation of antigen-specific naive CD8 T cells in response to low doses of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl061
更新日期:2006-08-01 00:00:00
abstract::We have previously shown that the occurrence of CD45RA+ adult mouse thymocytes is strain-dependent, e.g. constituting approximately 0.6% in C57BL/Icrf and approximately 2.5% in BALB/c (Huby, R. and Goff, L., 1992. Eur. J. Immunol. 22:1659). Here we show that irrespective of strain, the thymus contains approximately 0....
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pub_type: 杂志文章
doi:10.1093/intimm/4.11.1303
更新日期:1992-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxx004
更新日期:2017-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.10.1255
更新日期:2001-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:The pathogenesis of inflammatory skin disease involves the release of cytokines from keratinocytes, and one of these, IL-1β, has been previously implicated in inflammatory skin disease. T(h)17 cells, a subset of T(h) cells involved in autoimmunity and inflammation, possess IL-1β receptors and secrete cytokin...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxr110
更新日期:2012-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf110
更新日期:2002-12-01 00:00:00
abstract::Mouse CD4+ T cells were subdivided into two subpopulations, naive (CD44low CD45RBhigh) and memory (CD44high CD45RBlow) T cells, by flow cytometric analysis. Examination of spleen and peripheral blood of C57BL/6 mice of various ages revealed that there was a reciprocal age-associated change in these two subpopulations,...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.1.97
更新日期:1995-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.5.645
更新日期:1997-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt012
更新日期:2013-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.7.1011
更新日期:1994-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl006
更新日期:2006-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/13.6.733
更新日期:2001-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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更新日期:1997-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:International immunology
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doi:10.1093/intimm/dxv014
更新日期:2015-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.11.1263
更新日期:1992-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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doi:10.1093/intimm/6.6.795
更新日期:1994-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.3.245
更新日期:1991-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/13.4.411
更新日期:2001-04-01 00:00:00