Abstract:
:In this study we investigated the role of the invariant chain (li) in the presentation of hen egg lysozyme (HEL) and measles virus hemagglutinin (HA) antigens to MHC class II-restricted T hybridoma cells. Fibroblastic cells transfected with Ed or Ak genes, and supertransfected or not with the li gene, were used as antigen-presenting cells (APC). For every APC pair analysed, the amount of exogenous antigen needed to obtain a T-cell response was inversely correlated with the level of li expression. Exogenously provided HEL was efficiently presented by li-supertransfected APC at doses of 10 micrograms/ml or below. In contrast, non-li transfected fibroblastic cells, which express a low level of endogenous li, required at least 10 times more HEL to stimulate most of the T hybridoma cells. Analogous results were also obtained using exogenous HA. Finally, two different experiments suggest that basal li expressed in fibroblastic cells is involved in the presentation of exogenous antigen. In the first one, we showed that li/class II ratio was increased in high-density grown fibroblastic cells and that this increase correlates with the ability of the cells to present exogenous antigen. In the second, treating high-density grown cells with an antisense li oligodeoxynucleotide could impair their ability to present exogenous HEL. We also examined the presentation of endogenously-synthesized HEL or HA after introduction of the antigen into the biosynthetic pathway of the APC by transfection of HEL and HA cDNAs. There was no apparent difference in the capability of high density grown fibroblastic cells, transfected or not with li gene, to present endogenous HEL or HA.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Bertolino P,Forquet F,Pont S,Koch N,Gerlier D,Rabourdin-Combe Cdoi
10.1093/intimm/3.5.435subject
Has Abstractpub_date
1991-05-01 00:00:00pages
435-43issue
5eissn
0953-8178issn
1460-2377journal_volume
3pub_type
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