Correlation between invariant chain expression level and capability to present antigen to MHC class II-restricted T cells.


:In this study we investigated the role of the invariant chain (li) in the presentation of hen egg lysozyme (HEL) and measles virus hemagglutinin (HA) antigens to MHC class II-restricted T hybridoma cells. Fibroblastic cells transfected with Ed or Ak genes, and supertransfected or not with the li gene, were used as antigen-presenting cells (APC). For every APC pair analysed, the amount of exogenous antigen needed to obtain a T-cell response was inversely correlated with the level of li expression. Exogenously provided HEL was efficiently presented by li-supertransfected APC at doses of 10 micrograms/ml or below. In contrast, non-li transfected fibroblastic cells, which express a low level of endogenous li, required at least 10 times more HEL to stimulate most of the T hybridoma cells. Analogous results were also obtained using exogenous HA. Finally, two different experiments suggest that basal li expressed in fibroblastic cells is involved in the presentation of exogenous antigen. In the first one, we showed that li/class II ratio was increased in high-density grown fibroblastic cells and that this increase correlates with the ability of the cells to present exogenous antigen. In the second, treating high-density grown cells with an antisense li oligodeoxynucleotide could impair their ability to present exogenous HEL. We also examined the presentation of endogenously-synthesized HEL or HA after introduction of the antigen into the biosynthetic pathway of the APC by transfection of HEL and HA cDNAs. There was no apparent difference in the capability of high density grown fibroblastic cells, transfected or not with li gene, to present endogenous HEL or HA.(ABSTRACT TRUNCATED AT 250 WORDS)


Int Immunol


International immunology


Bertolino P,Forquet F,Pont S,Koch N,Gerlier D,Rabourdin-Combe C




Has Abstract


1991-05-01 00:00:00












  • Protective cytotoxic T lymphocyte responses against paramyxoviruses induced by epitope-based DNA vaccines: involvement of IFN-gamma.

    abstract::Plasmid DNA vectors have been constructed with minigenes encoding a single cytotoxic T lymphocyte (CTL) epitope from either the M2 protein of respiratory syncytial virus (RSV) or from the nucleoprotein of measles virus (MV) with or without a signal sequence (also called secretory or leader sequence). Following intrade...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hsu SC,Obeid OE,Collins M,Iqbal M,Chargelegue D,Steward MW

    更新日期:1998-10-01 00:00:00

  • S1P lyase in thymic perivascular spaces promotes egress of mature thymocytes via up-regulation of S1P receptor 1.

    abstract::Sphingosine 1-phosphate (S1P) and S1P receptor 1 (S1P1) play an important role in the egress of mature CD4 or CD8 single-positive (SP) thymocytes from the thymus. Fingolimod hydrochloride (FTY720), an S1P1 functional antagonist, induced significant accumulation of CD62L(high)CD69(low) mature SP thymocytes in the thymi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Maeda Y,Yagi H,Takemoto K,Utsumi H,Fukunari A,Sugahara K,Masuko T,Chiba K

    更新日期:2014-05-01 00:00:00

  • Production of IFN-γ by CD4(+) T cells in response to malaria antigens is IL-2 dependent.

    abstract::T-cell immune responses are critical for protection of the host and for disease pathogenesis during infection with Plasmodium species. We examined the regulation of CD4(+) T-cell cytokine responses during infection with Plasmodium berghei ANKA (PbA). CD4(+) T cells from PbA-infected mice produced IFN-γ, IL-4 and IL-10...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kimura D,Miyakoda M,Honma K,Shibata Y,Yuda M,Chinzei Y,Yui K

    更新日期:2010-12-01 00:00:00

  • Intestinal epithelial cell-derived IL-15 determines local maintenance and maturation of intra-epithelial lymphocytes in the intestine.

    abstract::Interleukin-15 (IL-15) is a cytokine critical for maintenance of intestinal intra-epithelial lymphocytes (IELs), especially CD8αα + IELs (CD8αα IELs). In the intestine, IL-15 is produced by intestinal epithelial cells (IECs), blood vascular endothelial cells (BECs) and hematopoietic cells. However, the precise role of...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Zhu Y,Cui G,Miyauchi E,Nakanishi Y,Mukohira H,Shimba A,Abe S,Tani-Ichi S,Hara T,Nakase H,Chiba T,Sehara-Fujisawa A,Seno H,Ohno H,Ikuta K

    更新日期:2020-05-08 00:00:00

  • MicroRNA 125b inhibition of B cell differentiation in germinal centers.

    abstract::MicroRNAs 125a and 125b are predicted to be able to bind to the B lymphocyte-induced maturation protein-1 (BLIMP-1) and IFN regulatory protein-4 (IRF-4) transcription factors, which are essential for plasma cell differentiation. A computational survey of the human and mouse genomes revealed that miR-125a and miR-125b ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Gururajan M,Haga CL,Das S,Leu CM,Hodson D,Josson S,Turner M,Cooper MD

    更新日期:2010-07-01 00:00:00

  • Cytokine dependence of V gamma 3 thymocytes: mature but not immature V gamma 3 cells require endogenous IL-2 and IL-7 to survive--evidence for cytokine redundancy.

    abstract::It has previously been described that V gamma 3 cells can proliferate extensively in vitro in the presence of different cytokines. Here, the role of cytokines in the maintenance of V gamma 3 cells in the thymus has been determined. Culture of fetal thymocytes in cell suspension for 24 h showed that, whereas immature T...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Leclercq G,De Smedt M,Plum J

    更新日期:1995-05-01 00:00:00

  • Differential expression of B7 co-stimulatory molecules by astrocytes correlates with T cell activation and cytokine production.

    abstract::Whether astrocytes utilize B7:CD28 co-stimulation to activate T cells mediating CNS inflammatory disease is controversial. In this report, primary astrocytes and murine astrocyte lines, generated by immortalization at two different times, day 7 or 45 of culture, were examined for their capability to express B7 co-stim...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Soos JM,Ashley TA,Morrow J,Patarroyo JC,Szente BE,Zamvil SS

    更新日期:1999-07-01 00:00:00

  • Immunomodulation by hepatitis C virus-derived proteins: targeting human dendritic cells by multiple mechanisms.

    abstract::Hepatitis C virus (HCV) has the ability to persist in the majority of infected people. Strong, multispecific and sustained T-cell response is correlated with viral clearance. The mechanisms of chronicity by HCV are unclear. HCV could restrain the immune system and establish chronic infection by modulating dendritic ce...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Krishnadas DK,Ahn JS,Han J,Kumar R,Agrawal B

    更新日期:2010-06-01 00:00:00

  • CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.

    abstract::Chemokine receptors (CCRs) are important co-stimulatory molecules found on many blood cells and associated with various diseases. The expression and function of CCRs on mast cells has been quite controversial. In this study, we report for the first time that murine bone marrow-derived mast cells (BMMC) express messeng...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Fifadara NH,Aye CC,Raghuwanshi SK,Richardson RM,Ono SJ

    更新日期:2009-08-01 00:00:00

  • Bystander apoptosis induced by CD8+ cytotoxic T cell (CTL) clones: implications for CTL lytic mechanisms.

    abstract::Cytotoxic T lymphocyte (CTL) mediated killing has classically been associated with a high level of specificity for the target cell. However, when CTL clones and specific target cells were copelleted with innocent bystander cells for which the CTL was not specific, these bystander cells were also lysed. Bystander lysis...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Burrows SR,Fernan A,Argaet V,Suhrbier A

    更新日期:1993-09-01 00:00:00

  • Reconstitution of the intermediate-affinity interleukin-2 receptor by cell fusion.

    abstract::Although IL-2 receptor beta chain (IL-2R beta) expressed in various lymphoid cell lines binds IL-2 with an intermediate affinity, IL-2R beta expressed in fibroblasts is unable to bind IL-2, suggesting that IL-2R beta is on its own not sufficient for generating the intermediate-affinity receptor and that lymphoid-speci...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kuida K,Tanaka T,Kitamura F,Toyama-Sorimachi N,Miyasaka M

    更新日期:1992-10-01 00:00:00

  • CD86 (B70/B7-2) on endothelial cells co-stimulates allogeneic CD4+ T cells.

    abstract::In vascularized organ transplantation, vascular endothelial cells (EC) confronting recipient T cells are potentially significant APC initiating cellular immune responses that lead to rejection. In the present study, we studied the ability of human EC to stimulate allogeneic T cells and the co-stimulatory molecules inv...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Seino K,Azuma M,Bashuda H,Fukao K,Yagita H,Okumura K

    更新日期:1995-08-01 00:00:00

  • Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists.

    abstract::NK cells limit the emergence of cancers and viral infections by surveillance of 'missing-self' and 'induced-self' ligands, and by direct recognition of pathogen-associated molecules. We examined individual roles for Toll-like receptors (TLRs)-7 and -8 in human NK-cell activation using synthetic, small molecule agonist...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Gorski KS,Waller EL,Bjornton-Severson J,Hanten JA,Riter CL,Kieper WC,Gorden KB,Miller JS,Vasilakos JP,Tomai MA,Alkan SS

    更新日期:2006-07-01 00:00:00

  • CXCR6 regulates the recruitment of pro-inflammatory IL-17A-producing T cells into atherosclerotic aortas.

    abstract::The adaptive immune response is involved in the development and progression of atherosclerosis and IL-17A(+) cells play a role in this disease. Although elevated number of CD4(+) IL-17A(+) (Th17) and IL-17A(+)TCRγδ(+) T cells are found within murine atherosclerotic aortas and human plaques, the mechanisms governing IL...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Butcher MJ,Wu CI,Waseem T,Galkina EV

    更新日期:2016-05-01 00:00:00

  • Characterization of the developmental landscape of murine RORγt+ iNKT cells.

    abstract::Invariant natural killer T (iNKT) cells expressing the retinoic acid receptor-related orphan receptor γt (RORγt) and producing IL-17 represent a minor subset of CD1d-restricted iNKT cells (iNKT17) in C57BL/6J (B6) mice. We aimed in this study to define the reasons for their low distribution and the sequence of events ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Klibi J,Li S,Amable L,Joseph C,Brunet S,Delord M,Parietti V,Jaubert J,Marie J,Karray S,Eberl G,Lucas B,Toubert A,Benlagha K

    更新日期:2020-02-07 00:00:00

  • The role of thymus in the aging of Th cell subpopulations and age-associated alteration of cytokine production by these cells.

    abstract::Mouse CD4+ T cells were subdivided into two subpopulations, naive (CD44low CD45RBhigh) and memory (CD44high CD45RBlow) T cells, by flow cytometric analysis. Examination of spleen and peripheral blood of C57BL/6 mice of various ages revealed that there was a reciprocal age-associated change in these two subpopulations,...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kurashima C,Utsuyama M,Kasai M,Ishijima SA,Konno A,Hirokawa K

    更新日期:1995-01-01 00:00:00

  • Stepwise formation of the high-affinity complex of the interleukin 2 receptor.

    abstract::The interleukin 2 receptor (IL-2R) is composed of at least two polypeptides--a 55 kd protein (the L chain, p55, or alpha chain) and a 75 kd protein (the H chain, p75 or beta chain). The high-affinity binding of IL-2 results in the formation of a ternary complex consisting of IL-2 and the L and H chains. We found that ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Saito Y,Ogura T,Kamio M,Sabe H,Uchiyama T,Honjo T

    更新日期:1990-01-01 00:00:00

  • Neutrophil activation and survival are modulated by interaction with NK cells.

    abstract::It is increasingly evident that neutrophils are able to cross-talk with other leukocytes to shape ongoing inflammatory and immune responses. In this study, we analyzed whether human NK cells may influence the survival and activation of neutrophils under co-culture conditions. We report that NK cells exposed to either ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Costantini C,Micheletti A,Calzetti F,Perbellini O,Pizzolo G,Cassatella MA

    更新日期:2010-10-01 00:00:00

  • Host chemokine (C-C motif) ligand-2 (CCL2) is differentially regulated in HIV type 1 (HIV-1)-infected individuals.

    abstract::Several cytokines and chemokines including chemokine (C-C motif) ligand-2 (CCL2) are induced in HIV-1 infection. However, the impact of HIV-1 viremia on CCL2 regulation is largely unknown. We utilized a DNA oligonucleotide microarray covering 110 inflammatory genes. Five genes were induced by at least 2-fold in PBMCs ...

    journal_title:International immunology

    pub_type: 临床试验,杂志文章


    authors: Ansari AW,Bhatnagar N,Dittrich-Breiholz O,Kracht M,Schmidt RE,Heiken H

    更新日期:2006-10-01 00:00:00

  • Direct binding of IL-12 to human and murine B lymphocytes.

    abstract::IL-12 has been shown to play a central role in cell-mediated inflammatory reactions through direct activation of T cells and NK cells. IL-12 also strongly influences humoral immunity but these effects have been thought to be indirect and caused by intermediary cytokines. Using flow cytometry, we now show that IL-12 di...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Vogel LA,Showe LC,Lester TL,McNutt RM,Van Cleave VH,Metzger DW

    更新日期:1996-12-01 00:00:00

  • Cellular selection leads to age-dependent and reversible down-regulation of transgenic immunoglobulin light chain genes.

    abstract::Analysis of immunoglobulin expression in mice transgenic for either a kappa light chain (driven by the kappa enhancer) or lambda light chain (driven by the IgH enhancer) revealed that the transgenic light chains are expressed by the majority of B lymphocytes in the neonatal mice. However, the proportion of B cells tha...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Pettersson S,Sharpe MJ,Gilmore DR,Surani MA,Neuberger MS

    更新日期:1989-01-01 00:00:00

  • Pyroptotic cells externalize eat-me and release find-me signals and are efficiently engulfed by macrophages.

    abstract::Pathogenic intracellular bacteria often hijack macrophages for their propagation. The infected macrophages release IL-1β and IL-18 and simultaneously commit suicide, which is called pyroptosis; both responses require caspase-1. Here, we found that pyroptotic cells induced by microbial infection were efficiently engulf...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Wang Q,Imamura R,Motani K,Kushiyama H,Nagata S,Suda T

    更新日期:2013-06-01 00:00:00

  • Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation.

    abstract::Although there has been extensive analysis on the capacity of MHC-peptide tetramers to bind antigen-specific TCR, there have been comparatively few studies regarding the role of the CD4 and CD8 co-receptors in binding and activation by these multimeric molecules. Here, we start from the observation that different anti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Campanelli R,Palermo B,Garbelli S,Mantovani S,Lucchi P,Necker A,Lantelme E,Giachino C

    更新日期:2002-01-01 00:00:00

  • Roles of Ets proteins, NF-kappa B and nocodazole in regulating induction of transcription of mouse germline Ig alpha RNA by transforming growth factor-beta 1.

    abstract::Antibody class switch recombination (CSR) occurs after antigen activation of B cells. CSR is directed to specific heavy chain isotypes by cytokines and B cell activators that induce transcription from the unrearranged, or germline (GL), C(H) region genes. Transforming growth factor (TGF)-beta1 is essential for switch ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Shi MJ,Park SR,Kim PH,Stavnezer J

    更新日期:2001-06-01 00:00:00

  • p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes.

    abstract::p40 was previously described as a regulatory molecule capable of inhibiting both the natural and the CD16-mediated cytotoxicity of NK cells. In this study, we analyze the effect of p40 molecule engagement on the NK cell triggering induced by activating HLA class I-specific NK receptors (NKR) or on TCR alpha beta-media...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Poggi A,Tomasello E,Revello V,Nanni L,Costa P,Moretta L

    更新日期:1997-09-01 00:00:00

  • Lymphotoxin and tumor necrosis factor-alpha production by myelin basic protein-specific T cell clones correlates with encephalitogenicity.

    abstract::Lymphokine activity in seven myelin basic protein (MBP)-specific T cell clones was examined. All of the clones recognize MBP peptide 1-9 in the context of I-Au. A strong positive correlation was found between levels of lymphotoxin (LT) and tumor necrosis factor alpha (TNF-alpha) mRNA and biological activity on L929 ce...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Powell MB,Mitchell D,Lederman J,Buckmeier J,Zamvil SS,Graham M,Ruddle NH,Steinman L

    更新日期:1990-01-01 00:00:00

  • Ubiquitin-like polypeptide inhibits the IgE response of lipopolysaccharide-activated B cells.

    abstract::Monoclonal non-specific suppressor factor (MNSF), a lymphokine produced by a murine hybridoma, was originally found to inhibit the generation of lipopolysaccharide (LPS)-induced Ig-secreting cells. Most recently, we demonstrated the recombinant form of the ubiquitin-like segment (rUbi-L) or MNSF beta, an isoform of MN...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nakamura M,Nagata T,Xavier M,Tanigawa Y

    更新日期:1996-11-01 00:00:00

  • Allorecognition of human neural stem cells by peripheral blood lymphocytes despite low expression of MHC molecules: role of TGF-beta in modulating proliferation.

    abstract::Neural stem cells (NSCs) transplantation has been proposed as a means of restoring damaged brain tissue, a possibility rendered more likely by reports of low NSCs immunogenicity in various experimental models because of low expression of MHC class I and II as well as co-stimulatory molecules. We investigated the immun...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ubiali F,Nava S,Nessi V,Frigerio S,Parati E,Bernasconi P,Mantegazza R,Baggi F

    更新日期:2007-09-01 00:00:00

  • Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage.

    abstract::cDNA clones which strongly hybridized with a 3.1 kb mRNA from mouse macrophages and macrophage cell lines and weakly with mRNA from P815 but not from a variety of other cell lines and tissues were isolated from cDNA libraries constructed using mRNA from murine macrophage cell lines and peritoneal macrophages. Treatmen...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yoshida S,Setoguchi M,Higuchi Y,Akizuki S,Yamamoto S

    更新日期:1990-01-01 00:00:00

  • Modulation of the TCR stimulation strength can render human activated CD4+ T cells suppressive.

    abstract::In this study, we explored the potential of human naive CD4(+) T cells to acquire regulatory properties upon stimulation. We demonstrated that, in vitro, pre-activated naive CD4(+)CD25(-)CD45RA(+) T cells could become anergic and suppressive CD4(+)CD25(+) T cells upon lower intensity TCR stimulation. These CD4(+)CD25(...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Noël G,Brinster C,Semana G,Bruniquel D

    更新日期:2009-09-01 00:00:00