Abstract:
:In this study we investigated the role of the invariant chain (li) in the presentation of hen egg lysozyme (HEL) and measles virus hemagglutinin (HA) antigens to MHC class II-restricted T hybridoma cells. Fibroblastic cells transfected with Ed or Ak genes, and supertransfected or not with the li gene, were used as antigen-presenting cells (APC). For every APC pair analysed, the amount of exogenous antigen needed to obtain a T-cell response was inversely correlated with the level of li expression. Exogenously provided HEL was efficiently presented by li-supertransfected APC at doses of 10 micrograms/ml or below. In contrast, non-li transfected fibroblastic cells, which express a low level of endogenous li, required at least 10 times more HEL to stimulate most of the T hybridoma cells. Analogous results were also obtained using exogenous HA. Finally, two different experiments suggest that basal li expressed in fibroblastic cells is involved in the presentation of exogenous antigen. In the first one, we showed that li/class II ratio was increased in high-density grown fibroblastic cells and that this increase correlates with the ability of the cells to present exogenous antigen. In the second, treating high-density grown cells with an antisense li oligodeoxynucleotide could impair their ability to present exogenous HEL. We also examined the presentation of endogenously-synthesized HEL or HA after introduction of the antigen into the biosynthetic pathway of the APC by transfection of HEL and HA cDNAs. There was no apparent difference in the capability of high density grown fibroblastic cells, transfected or not with li gene, to present endogenous HEL or HA.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Bertolino P,Forquet F,Pont S,Koch N,Gerlier D,Rabourdin-Combe Cdoi
10.1093/intimm/3.5.435subject
Has Abstractpub_date
1991-05-01 00:00:00pages
435-43issue
5eissn
0953-8178issn
1460-2377journal_volume
3pub_type
杂志文章abstract::The human IL-3 gene, located on chromosome 5, contains several cis-acting DNA sequences, i.e. CLE (conserved lymphokine element) and a GC-rich region, similar to the GM-CSF gene. To investigate the role of these elements, the 5' flanking region of the IL-3 gene was attached to a bacterial chloramphenicol acetyltransfe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.3.245
更新日期:1991-03-01 00:00:00
abstract::NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor α chain encoded by Vα14Jα18 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analys...
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doi:10.1093/intimm/dxu057
更新日期:2014-10-01 00:00:00
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journal_title:International immunology
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更新日期:2000-09-01 00:00:00
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journal_title:International immunology
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doi:10.1093/intimm/4.8.955
更新日期:1992-08-01 00:00:00
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更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/9.7.1011
更新日期:1997-07-01 00:00:00
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更新日期:2016-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg006
更新日期:2003-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh149
更新日期:2004-10-01 00:00:00
abstract::Rheumatic fever (RF) follows a throat infection with different M-serotypes of beta-hemolytic group A streptococci (GAS) and can affect different tissues, predominantly the heart. It is thought to be an autoimmune illness. Although histological examination of affected heart shows an infiltrate consisting mainly of T ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1235
更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/8.11.1751
更新日期:1996-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1229
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy065
更新日期:2019-02-06 00:00:00
abstract::Natural cytotoxicity receptors and NKG2D correspond to major activating receptors involved in triggering of tumor cell lysis by human NK cells. In this report, we investigated the expression of NKG2D ligands (NKG2DLs), MHC class I-related chain (MIC) A, MICB and UL16-binding proteins 1, 2 and 3, on a panel of human no...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn038
更新日期:2008-07-01 00:00:00
abstract::Blocking T cell co-stimulatory signals by anti-B7-1/B7-2 mAb is an attractive approach to treat autoimmune diseases. However, anti-B7-1/B7-2 mAb treatment is known to exacerbate autoimmune diseases through mechanisms not fully understood. Tumor necrosis factor alpha (TNF-alpha) and reactive oxygen species (ROS) also p...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm012
更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:International immunology
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doi:10.1093/intimm/4.3.321
更新日期:1992-03-01 00:00:00
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更新日期:1995-11-01 00:00:00
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doi:10.1093/intimm/2.12.1203
更新日期:1990-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.3.353
更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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更新日期:2019-05-21 00:00:00
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更新日期:2004-12-01 00:00:00
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更新日期:2009-01-01 00:00:00
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更新日期:2017-02-01 00:00:00