Abstract:
:In vascularized organ transplantation, vascular endothelial cells (EC) confronting recipient T cells are potentially significant APC initiating cellular immune responses that lead to rejection. In the present study, we studied the ability of human EC to stimulate allogeneic T cells and the co-stimulatory molecules involved in this response. On both human umbilical vein endothelial cells (HUVEC) and microvascular endothelial cells (MVEC), MHC class I, intercellular adhesion molecule (ICAM)-1 and CD86 were constitutively expressed as assessed by flow cytometry. After IFN-gamma treatment, MHC class II expression was induced, and MHC class I and ICAM-1 were up-regulated. In contrast, the expression of CD86 was unchanged and CD80 was undetectable even after IFN-gamma treatment. Highly purified CD4+ T cells proliferated in response to IFN-gamma-treated allogeneic HUVEC and MVEC, and this response was efficiently blocked by mAb to MHC class II, ICAM-1 and CD86. Furthermore, the addition of anti-CD86 mAb to the primary culture with allogeneic EC resulted in the induction of alloantigen-specific anergy. These results suggest that CD86 expressed on EC plays a critical role in initiating cellular immune responses to vascularized allografts and would be an important target for immune intervention.
journal_name
Int Immunoljournal_title
International immunologyauthors
Seino K,Azuma M,Bashuda H,Fukao K,Yagita H,Okumura Kdoi
10.1093/intimm/7.8.1331subject
Has Abstractpub_date
1995-08-01 00:00:00pages
1331-7issue
8eissn
0953-8178issn
1460-2377journal_volume
7pub_type
杂志文章abstract::Previously we observed elevation of the serum concentration of two acute-phase protein (AFP) complement components (C9 and C1-inhibitor) in patients with chronic hepatitis C who responded (R) to IFN-alpha therapy, but not in non-responders (NR). In the present study we investigated the effect of high-dose IFN-alpha th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh024
更新日期:2004-01-01 00:00:00
abstract::It is well established that the bacterial superantigens bind to both TCR beta-chain and, with moderate affinity, to MHC class II molecules. Class II-bearing cells bind the superantigen and present the superantigen to T cells expressing certain TCR beta-chain variable region alleles. We have observed that the superanti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.11.1721
更新日期:1995-11-01 00:00:00
abstract::IL-12 has been shown to play a central role in cell-mediated inflammatory reactions through direct activation of T cells and NK cells. IL-12 also strongly influences humoral immunity but these effects have been thought to be indirect and caused by intermediary cytokines. Using flow cytometry, we now show that IL-12 di...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.12.1955
更新日期:1996-12-01 00:00:00
abstract::Fc receptors play important roles for a wide array of immune responses. In contrast to the well-defined Fcγ and Fcε receptors, the molecular and functional characteristics of Fc receptors for IgA and IgM have remained incompletely understood for years. Recent progress has unveiled the characteristics of Fc receptors f...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx059
更新日期:2017-12-30 00:00:00
abstract::The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneo...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh143
更新日期:2004-10-01 00:00:00
abstract::Malaria infection induces the production of serum antibodies to a variety of malaria antigens but the prevalence of antibodies to any particular antigen is typically much less than 100%. It has been assumed that non-responsiveness to defined antigens in malaria immune subjects is due to HLS-mediated restriction of the...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.905
更新日期:1996-06-01 00:00:00
abstract::After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and re...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm086
更新日期:2007-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.3.401
更新日期:1995-03-01 00:00:00
abstract::The immediate responses to aerosolized staphylococcal enterotoxin B (SEB) in respiratory toxic shock were studied in the circulation of rhesus monkeys with low antibody levels following immunization with SEB toxoid-containing microspheres. Both the surviving and dying monkeys had toxic shock syndrome 4-48 h after SEB ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.12.1825
更新日期:1997-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxy067
更新日期:2019-02-15 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.6.509
更新日期:1990-01-01 00:00:00
abstract::Src homology region 2-domain-containing phosphatase-1 (SHP-1) plays an important role in the regulation of signaling from various receptors in hematopoietic cells. In mast cells, SHP-1 has been shown to negatively regulate the initial signaling triggered by high-affinity receptor for IgE (FcepsilonRI) and positively r...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm070
更新日期:2007-09-01 00:00:00
abstract::The utilization of individual VH7183 genes in the autoimmune non-obese diabetic (NOD) mouse and the normal C57BL/6 mouse, both carrying the IgHb haplotype, was investigated. Nucleotide sequence analyses of a large number of VHDJH rearrangements utilizing VH7183 gene segments allowed us to identify 10 individual member...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.4.623
更新日期:1994-04-01 00:00:00
abstract::We have previously cloned the murine homolog of cDNA for the human myelomonocytic differentiation antigen, CD14. We synthesized three hydrophilic peptides derived from the predicted amino acid sequence of murine CD14 (mCD14), designated MS7.1, MS7.2, and MS7.3, respectively, and raised antisera against them. Each anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.2.205
更新日期:1991-02-01 00:00:00
abstract::Dendritic cells (DCs) are responsible for the initiation of immune responses. Our study demonstrates a new pathway for generating a large quantity of stimulatory monocyte-derived DCs (Mo-DCs) from human monocytes using anti-4-1BB ligand (4-1BBL) mAb to trigger reverse signaling. The anti-4-1BBL-driven Mo-DCs (DCs(alph...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp077
更新日期:2009-10-01 00:00:00
abstract::The non-obese diabetic (NOD) mouse spontaneously develops T-cell-mediated autoimmune insulitis. We analyzed the clonotypes of T cell infiltrates of the NOD mouse islets using a new method we have developed recently, which consists of RT-PCR amplification of the CDR3 region of the TCR beta chain mRNA and subsequent sin...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.807
更新日期:1996-06-01 00:00:00
abstract::The B cell-activating factor from the tumor necrosis factor family (BAFF) is an important regulator of B cell immunity. Recently, we demonstrated that recombinant BAFF also provides a co-stimulatory signal to T cells. Here, we studied expression of BAFF in peripheral blood leukocytes and correlated this expression wit...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh043
更新日期:2004-03-01 00:00:00
abstract::Three MHC class II-derived synthetic peptides (I-A beta (g7)1-16, I-A beta (g7)52-77 and I-A alpha (g7)63-82YC) were analyzed for their ability to bind to syngeneic and allogeneic MHC class II molecules using a whole cell, competitive peptide binding assay. These studies demonstrated that the A beta (g7)1-16 peptide w...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.12.1857
更新日期:1996-12-01 00:00:00
abstract::The alternative complement pathway (ACP) in vertebrates is known to be important in inflammatory reactions, and to be activated by foreign substances such as bacterial lipopolysaccharide (LPS) and zymosan, although to date no intrinsic activators have been identified except complement receptor type 2. From the point o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1349
更新日期:1993-10-01 00:00:00
abstract::We have recently described the novel A6H antigen expressed on human peripheral blood T cells and on renal cell carcinoma cells. Cross-linking of the A6H antigen results in co-stimulation of human CD4(+) T cells, characterized by induction of the transcription factor activator protein-1 (AP-1), proliferation and promin...
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pub_type: 杂志文章
doi:10.1093/intimm/12.3.253
更新日期:2000-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.11.1777
更新日期:1994-11-01 00:00:00
abstract::NOD mice spontaneously develop type I diabetes resulting from autoimmune destruction of their insulin-producing beta cells. Among the self-antigens targeted by NOD autoimmune T cells is a peptide, p277, from the sequence of the 60 kDa heat shock protein (hsp60). Common to the anti-p277 T cell populations of NOD mice i...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.6.957
更新日期:1999-06-01 00:00:00
abstract::We have searched the human genome for genes that predispose to rheumatoid arthritis (RA) using fluorescence-based microsatellite marker analysis and affected sib-pair linkage study. A panel of 41 Japanese families, each with at least two affected siblings, was typed for genome-wide 358 polymorphic microsatellite marke...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.12.1891
更新日期:1998-12-01 00:00:00
abstract::We have followed CD4 and CD8 TCR V beta repertoires during the acute phase of Trypanosoma cruzi infection in a resistant mouse strain (C57BL/6). No major changes were found in the V beta TCR distributions analyzed (covering roughly 40% of the TCR repertoire) in peripheral CD4 T lymphocytes, confirming the polyclonal n...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.3.387
更新日期:1994-03-01 00:00:00
abstract::NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor α chain encoded by Vα14Jα18 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analys...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu057
更新日期:2014-10-01 00:00:00
abstract::Previously we examined the ability of the host's immune responses to regulate Ig production in an IgE-secreting murine plasma cell tumor (B53). In the present study we have examined the reverse phenomenon, in that we have investigated the effects of this and other plasma cell tumors on the immune responses of their ho...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1029
更新日期:1995-07-01 00:00:00
abstract::The kinetics of kappa light (kappa L) chain gene rearrangement and expression on mRNA and protein level has been studied with four stromal cell/IL-7 reactive, long-term in vitro proliferating pre-B cell lines and clones, two from fetal liver of normal mice and two from fetal liver of E microH-bcl-2 transgenic (bcl-2-t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.12.1609
更新日期:1993-12-01 00:00:00
abstract::Secretory IgA (sIgA) antibodies in human tears and milk were found to recognize protein disulfide isomerase (PDI) on a Toxoplasma gondii lysate immunoblot (IB). These antibodies were already detectable in tears of infants. To determine the epitope containing-regions on PDI, we generated truncated versions of recombina...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf091
更新日期:2002-11-01 00:00:00
abstract::After a 3 week course (approximately), during which there is marked lymphoid hyperplasia, Trypanosoma musculi infections in young-adult mice are cured by an immune mechanism involving antibodies of the IgG2a isotype. Both the lymphoid hyperplasia and IgG2a antibody response are T-cell-dependent events and both process...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1107
更新日期:1994-08-01 00:00:00
abstract::Dendritic cells (DCs) are specialized antigen-presenting cells that play pivotal roles in initiating immune responses. However, DC maturation is usually strongly restricted by the stromal microenvironment, especially in non-lymphoid tissues, such as skin and mucosa. Although suppression of DC maturation by stromal cel...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn078
更新日期:2008-09-01 00:00:00