Abstract:
:Using Ia antigen-containing lipid vesicles, we investigated by stopped-flow fluorometry the requirements for helper T cell recognition and activation. When azobenzenearsonate-L-tyrosine (ABA-L-Tyr)-specific, I-Ak-restricted helper T cell hybridomas 2-45-12 were mixed with ABA-L-Tyr and purified I-Ak molecules on lipid vesicles, an increase of intercellular calcium ion concentration ([Ca2+]i) in the T cells were detected within 1-2s. The average increases of [Ca2+]i were not much different when the lipid vesicles were composed of dimyristoylphosphatidylcholine or of egg phosphatidylcholine, but they were dependent on the density of I-Ak molecules on the liposomes. The increase of [Ca2+]i was inhibited in the presence of anti-I-Ak monoclonal antibody 10.2.16, but not by the addition of anti-L3T4 monoclonal antibody GK1.5. However, the addition of anti-L3T4 antibody during the first 3 h of cultivation completely inhibited the T cell activation [interleukin (IL-2) production]. In our experimental system, IL-2 production was observed either when L3T4-positive T cell hybridomas 2-45-12 were stimulated with ABA-L-Tyr and Ia molecules on the vesicles in the presence of phorbol 12-myristate 13-acetate, or when L3T4-negative T cell hybridomas 3H60.12 were incubated with ABA-L-Tyr and Ia molecules on the planar membranes.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Odaka C,Utsunomiya N,Nakanishi M,Arata Y,Manabe S,Yasuda T,Tadakuma Tdoi
10.1093/intimm/2.6.509subject
Has Abstractpub_date
1990-01-01 00:00:00pages
509-14issue
6eissn
0953-8178issn
1460-2377journal_volume
2pub_type
杂志文章abstract::Invariant natural killer T (iNKT) cells expressing the retinoic acid receptor-related orphan receptor γt (RORγt) and producing IL-17 represent a minor subset of CD1d-restricted iNKT cells (iNKT17) in C57BL/6J (B6) mice. We aimed in this study to define the reasons for their low distribution and the sequence of events ...
journal_title:International immunology
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更新日期:2012-03-01 00:00:00
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journal_title:International immunology
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更新日期:2003-01-01 00:00:00
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更新日期:1996-04-01 00:00:00
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更新日期:2004-09-01 00:00:00
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更新日期:2005-08-01 00:00:00
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doi:10.1093/intimm/12.9.1347
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更新日期:2008-02-01 00:00:00
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更新日期:2007-09-01 00:00:00
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更新日期:2000-10-01 00:00:00
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