Abstract:
:Incomplete T cell antigen receptor-mediated signaling induces an unresponsive state known as anergy. Previously, we had shown that anergy can be induced in antigen-primed but not naive T cells. In this report, we found that in vitro primed T cells from IL-2R alpha-deficient mice were resistant to anergy induction in contrast to comparably treated wild-type T cells. This resistance persisted even after proliferation of IL-2R alpha chain-deficient CD4 T cells with high-dose IL-2-IL-2R beta gamma chains interaction. Thus, antigen activation, and/or progression through cell cycle are not sufficient to induce anergy susceptibility in T cells. The high-affinity IL-2-IL-2R interaction appears to play a critical role in this process.
journal_name
Int Immunoljournal_title
International immunologyauthors
Hayashi RJ,Kanagawa Odoi
10.1093/intimm/dxh397subject
Has Abstractpub_date
2006-05-01 00:00:00pages
645-51issue
5eissn
0953-8178issn
1460-2377pii
dxh397journal_volume
18pub_type
杂志文章abstract::CD25(+)CD4(+) regulatory T cells inhibit the activation of autoreactive T cells in vitro and in vivo, and suppress organ-specific autoimmune diseases. The mechanism of CD25(+)CD4(+) T cells in the regulation of experimental autoimmune encephalomyelitis (EAE) is poorly understood. To assess the role of CD25(+)CD4(+) T ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh029
更新日期:2004-02-01 00:00:00
abstract::Mice lacking the IL-2 receptor beta chain (IL-2R beta) exhibit an autoimmune reaction characterized by generalized T cell activation, production of autoantibodies, myeloproliferation and severe anemia. T cells of IL-2R beta-/- mice were examined to elucidate the mechanism responsible for their abnormal activation and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.9.1367
更新日期:1997-09-01 00:00:00
abstract::Previously we examined the ability of the host's immune responses to regulate Ig production in an IgE-secreting murine plasma cell tumor (B53). In the present study we have examined the reverse phenomenon, in that we have investigated the effects of this and other plasma cell tumors on the immune responses of their ho...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1029
更新日期:1995-07-01 00:00:00
abstract::The integrins have been grouped into three subfamilies based on the utilization of three distinct beta chain subunits, beta 1, beta 2, and beta 3. The recent discovery of beta 4 and beta 5 subunits, and the sharing of alpha subunits between beta subfamilies reflects a greater structural and functional diversity of the...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.11.1097
更新日期:1990-01-01 00:00:00
abstract::We have previously shown that the occurrence of CD45RA+ adult mouse thymocytes is strain-dependent, e.g. constituting approximately 0.6% in C57BL/Icrf and approximately 2.5% in BALB/c (Huby, R. and Goff, L., 1992. Eur. J. Immunol. 22:1659). Here we show that irrespective of strain, the thymus contains approximately 0....
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.11.1303
更新日期:1992-11-01 00:00:00
abstract::We have used a set of single-chain variable fragment antibodies (sc) genetically fused with an influenza hemagglutinin-derived peptide as a means to investigate the role of CR1 and CR2 in antigen presentation by B cells. When incubated with the B cell lymphoma 2PK3, peptide-containing sc specific for either CR1 or CR1...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.3.241
更新日期:2002-03-01 00:00:00
abstract::HLA class II molecules present superantigens more efficiently than their murine counterpart. Therefore, transgenic mice expressing HLA-DQ8 with and without CD28 were used to address the role of CD28 in staphylococcal enterotoxin B (SEB)-driven immune responses. SEB-induced in vitro proliferation of naive DQ8.CD28(-/-)...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf047
更新日期:2002-07-01 00:00:00
abstract::The ability of Bcl-2 to inhibit apoptotic cell death is well established. Several homologues of the bcl-2 gene, such as bax, bcl-x or mcl-1, have recently been identified. Like Bcl-2, both Bcl-XL and Mcl-1 appear to function as repressors of apoptotic cell death, whereas Bax facilitates it, indicating possible interac...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.11.1817
更新日期:1995-11-01 00:00:00
abstract::Investigation of the process of Peyer's patch (PP) formation has been hampered by difficulties in identifying its initial step in the embryo. In this study, we overcame this problem by means of whole-mount immunohistochemistry using mAb against the molecules which were expressed in the cells accumulated at the site of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.4.507
更新日期:1997-04-01 00:00:00
abstract::Recent studies point to glycolipid-enriched membrane (GEM) microdomains as the critical sites for TCR-mediated signal transduction. However, whether the TCR complex is localized in the GEM domain is not well-defined. In the present study, we analyzed localization of the TCR-CD3 complex in the GEM domain by isolating t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1395
更新日期:1999-09-01 00:00:00
abstract::After a 3 week course (approximately), during which there is marked lymphoid hyperplasia, Trypanosoma musculi infections in young-adult mice are cured by an immune mechanism involving antibodies of the IgG2a isotype. Both the lymphoid hyperplasia and IgG2a antibody response are T-cell-dependent events and both process...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1107
更新日期:1994-08-01 00:00:00
abstract::Naturally occurring CD4(+)CD25(+) regulatory T cells (nT(R)) comprise a separate lineage of T cells that are essential for maintaining immunological tolerance to self. Here we demonstrate that the level of phosphorylation of the TCR zeta-chain is approximately 1.5- to 4-fold higher in nT(R) as compared with CD4(+)CD25...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm021
更新日期:2007-04-01 00:00:00
abstract::Ig are multifunctional molecules with distinct properties assigned to individual domains. To assess the importance of IgM domain assembly in B cell development we generated two transgenic mouse lines with truncated muH chains by homologous integration of the neomycin resistance gene (neo(r)) into exons C(mu)1 and C(mu...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.12.1489
更新日期:2001-12-01 00:00:00
abstract::The thymus leukemia antigens (TL) belong to the MHC class Ib family and can be recognized by CD8-dependent or -independent cytotoxic T lymphocytes (CTL) showing TL, but not H-2, restriction. We previously reported that the CTL epitope is TAP independent and in the present study we further characterize the recognition ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg131
更新日期:2003-11-01 00:00:00
abstract::Acute graft-versus-host disease (GvHD) is a serious complication after allogeneic bone marrow transplantation. Donor-derived T cells infiltrate recipient target organs and cause severe tissue damage, often leading to death of the affected patient. Tissue destruction is a direct result of donor CD8+ T cell activation a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.5.503
更新日期:2002-05-01 00:00:00
abstract::Toll-like receptor 7 (TLR7) and TLR9 sense microbial single-stranded RNA (ssRNA) and ssDNA in endolysosomes. Nucleic acid (NA)-sensing in endolysosomes is thought to be important for avoiding TLR7/9 responses to self-derived NAs. Aberrant self-derived NA transportation to endolysosomes predisposes to autoimmune diseas...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu086
更新日期:2015-02-01 00:00:00
abstract::Although there has been extensive analysis on the capacity of MHC-peptide tetramers to bind antigen-specific TCR, there have been comparatively few studies regarding the role of the CD4 and CD8 co-receptors in binding and activation by these multimeric molecules. Here, we start from the observation that different anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.1.39
更新日期:2002-01-01 00:00:00
abstract::In order to better understand the role of intestinal CD1d, we sought to define the cellular localization and further characterize the biochemical structure of CD1d in human intestinal epithelial cells (IEC). Using a CD1d-specific rabbit anti-gst-CD1d antibody, immunoprecipitation of radiolabeled cell surface proteins ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.3.383
更新日期:1999-03-01 00:00:00
abstract::BALB/c mice are susceptible to cutaneous leishmaniasis upon infection with Leishmania major while C57BL/6 are not. There is a major promastigote surface protease (PSP or gp63) which is available in both native and recombinant forms, and for which the primary amino acid sequence is known. Immunization with PSP has been...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.5.785
更新日期:1994-05-01 00:00:00
abstract::Epitope spreading has been best characterized as an exacerbating factor in CD4(+) T cell-dependent autoimmune disease models and is believed to occur via presentation of antigens liberated by tissue destruction initiated by CD4(+) T cells specific for a primary epitope. The growing evidence that exogenous antigens can...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.5.625
更新日期:2001-05-01 00:00:00
abstract::Constitutive expression of p35 and p40 IL-12 mRNA was detected in splenic macrophages isolated from aged mice. Macrophages were also implicated as the cell type responsible for the dysregulated IL-6 and tumor necrosis factor (TNF)-alpha commonly observed to be constitutively produced by lymphoid cells from aged donors...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.5.745
更新日期:1997-05-01 00:00:00
abstract::We have previously reported that tumor necrosis factor receptor-associated factor 1 (TRAF1), an intracellular protein, which binds to a range of molecules, including tumor necrosis factor (TNF) receptor family members, regulates TNF-induced NF-kappaB and AP-1 signaling as well as TCR-triggered proliferative responses ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh354
更新日期:2006-01-01 00:00:00
abstract::After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and re...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm086
更新日期:2007-10-01 00:00:00
abstract::CD4 T cells are not thought to play a significant role in generating an effective primary CD8 T cell response to most viral infections. We have challenged this view by demonstrating that antigen-specific CD4 T cells can indeed suppress the proliferation of antigen-specific naive CD8 T cells in response to low doses of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl061
更新日期:2006-08-01 00:00:00
abstract::Treatment of melanoma cell lines with IFN-gamma induces the switch from proteasome (PS) to immunoproteasome (iPS). This finding has profound implications for the immunobiology of melanoma cells since certain peptides (such as Melan-A(mart1)(27-35)) are cleaved differently by iPS, thus implying a different ability to b...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg140
更新日期:2003-12-01 00:00:00
abstract::Spectroscopic methods have been applied to elucidate conformational differences responsible for the immunological diversity of two synthetic multichain copolymers, Tyr1Tyr2Glu3Glu4-poly-DL-Ala--poly-Lys and Tyr1Glu2Tyr3Glu4-poly-DL-Ala--poly-Lys. Despite their far-reaching structural similarity in the epitope peptide ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.591
更新日期:1993-06-01 00:00:00
abstract::Although CD4(+) T cells form a major subset of TCRalphabeta T cells, only a small number of TCRgammadelta T cells express CD4. Factors contributing to the down-regulation of CD4(+) TCRgammadelta T cells have not been identified. The CD5 molecule is expressed on most TCRgammadelta T cells in the spleen, whereas only a ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg006
更新日期:2003-01-01 00:00:00
abstract::Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.7.691
更新日期:1991-07-01 00:00:00
abstract::Antigen-driven interaction of dendritic cells (DC) with CD4(+) T(h) cells results in the exchange of bidirectional activating signals. Cross-linking of TCR by MHC class II-bound antigen activates T(h) cells, resulting in their up-regulation of CD40 ligand. Here we show that MHC class II molecules, in addition to their...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf073
更新日期:2002-09-01 00:00:00
abstract::Chemokine receptors (CCRs) are important co-stimulatory molecules found on many blood cells and associated with various diseases. The expression and function of CCRs on mast cells has been quite controversial. In this study, we report for the first time that murine bone marrow-derived mast cells (BMMC) express messeng...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp066
更新日期:2009-08-01 00:00:00