Abstract:
:Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, all V gamma 9-V delta 2 gamma delta T cells reportedly express CD45RO whereas all V delta gamma delta T cells lack CD45RO. Here, we show that these CD45RO- V delta gamma delta T cells all express CD45RA and the CD45RO+ V.9-V delta 2 gamma delta cells lack expression of CD45RA. The V delta T cells acquired CD45RO expression and lost part of their surface CD45RA, following in vitro activation with phytohaemagglutinin or IL-2. Also the CD3-CD16+ NK cells in peripheral blood that are uniformly CD45RA+ CD45RO- completely converted to the CD45RA-CD45RO+ phenotype upon in vitro activation. Moreover, all cloned V.9-V delta 2 and V delta 1 T cells and NK cells express CD45RO and lack expression of CD45RA. Our results strongly suggest that CD45RA and CD45RO are genuine markers for naive and primed lymphocytes that represent distinct differentiation lineages.
journal_name
Int Immunoljournal_title
International immunologyauthors
Braakman E,Sturm E,Vijverberg K,van Krimpen BA,Gratama JW,Bolhuis RLdoi
10.1093/intimm/3.7.691subject
Has Abstractpub_date
1991-07-01 00:00:00pages
691-7issue
7eissn
0953-8178issn
1460-2377journal_volume
3pub_type
杂志文章abstract::Immunoglobulin (IG:) genes are hypermutated in mature B cells after interaction with antigen and T cells in a germinal center reaction. We and others have recently shown that the human BCL6 gene is also hypermutated in human peripheral blood memory B cells and tonsils. A preliminary analysis of other non-Ig genes (c-M...
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更新日期:1995-05-01 00:00:00
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更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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更新日期:1995-03-01 00:00:00
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更新日期:1993-04-01 00:00:00
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