CD21 and CD62L shedding are both inducible via P2X7Rs.


:Neutrophils and lymphocytes are recruited to sites of inflammation and require the adhesion molecule L-selectin (CD62L) for adherence to endothelial cells. Nucleotides released from activated or dying cells at sites of inflammation can mediate signaling through purinergic receptor family II, resulting in CD62L shedding. Activation of B lymphocytes requires the complement receptor type II (CD21) and at the same time leads to shedding of CD21. Both CD62L and CD21 shedding possibly depends on the same families of proteases. In the present study, we characterized peripheral blood naive and memory cells and neutrophils for CD62L surface expression and analyzed benzoyl-benzoyl triphosphate (BzATP)-induced shedding. BzATP is able to induce CD62L shedding in naive and memory lymphocytes, but not in neutrophils. CD21 shedding can be induced through activation of the B cell receptor (BCR) or with mitogens. Here we show that CD21 is also susceptible to BzATP-induced shedding on peripheral B cells. In addition, using receptor inhibitors, we show that shedding of CD21 and CD62L is mediated via the P2X7R. P2X7R-mediated CD62L and CD21 shedding could occur as a result of extracellular accumulated ATP and may have an influence on leukocyte migrational behavior and BCR-mediated signaling.


Int Immunol


International immunology


Sengstake S,Boneberg EM,Illges H




Has Abstract


2006-07-01 00:00:00














  • Lipid mediators and sterile inflammation in ischemic stroke.

    abstract::Stroke is one of the major causes of lethality and disability, yet few effective therapies have been established for ischemic stroke. Inflammation in the ischemic brain is induced by the infiltration and subsequent activation of immune cells. Loss of cerebral blood flow and ischemic brain-cell death trigger the activa...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nakamura A,Otani K,Shichita T

    更新日期:2020-10-20 00:00:00

  • Neutrophil activation and survival are modulated by interaction with NK cells.

    abstract::It is increasingly evident that neutrophils are able to cross-talk with other leukocytes to shape ongoing inflammatory and immune responses. In this study, we analyzed whether human NK cells may influence the survival and activation of neutrophils under co-culture conditions. We report that NK cells exposed to either ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Costantini C,Micheletti A,Calzetti F,Perbellini O,Pizzolo G,Cassatella MA

    更新日期:2010-10-01 00:00:00

  • Immune checkpoint inhibition in ovarian cancer.

    abstract::Recent studies have shown that tumor cells acquire escape mechanisms to evade host immunity in the tumor microenvironment. Two key immune checkpoint pathways mediated by immunosuppressive co-signaling, the first via programmed cell death 1 (PD-1) and PD-1 ligand 1 (PD-1/PD-L1) and the second via CTLA-4 and B7 (CTLA-4/...

    journal_title:International immunology

    pub_type: 杂志文章,评审


    authors: Hamanishi J,Mandai M,Konishi I

    更新日期:2016-07-01 00:00:00

  • Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.

    abstract::Notch2 and Delta-like 1 (Dll1) have been implicated in the development of marginal zone B (MZB) cells. In the present study, we characterized the expression and function of mouse Notch receptors and ligands in the spleen by using newly generated mAbs. Although Notch2 was expressed on both B and T cells in the spleen, ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Moriyama Y,Sekine C,Koyanagi A,Koyama N,Ogata H,Chiba S,Hirose S,Okumura K,Yagita H

    更新日期:2008-06-01 00:00:00

  • Ig heavy chain class switching in Rag-deficient mice.

    abstract::To investigate potential roles of the RAG-1 and RAG-2 gene products in Ig heavy chain class recombination (CSR), we have generated RAG-1(-/-) and RAG-2(-/-) mice which contain both a rearranged Ig HC V(D)J gene (referred to as B1-8) inserted into the endogenous Ig heavy chain (HC) locus in place of the JH segments, an...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lansford R,Manis JP,Sonoda E,Rajewsky K,Alt FW

    更新日期:1998-03-01 00:00:00

  • TCR v(beta) usage and clonality of T cells isolated from progressing and rejected tumor sites before and after in vitro culture.

    abstract::A gelatin sponge model of concomitant tumor immunity was employed in order to examine the clonality of T cells associated with progressing and rejected tumor sites. Here we show that freshly isolated T cells bearing TCR V(beta)1, CDR3 RPGTGN, J(beta)1.1 and TCR V(beta)8, CDR3 GD, J(beta)1.6 predominated progressing an...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kurt RA,Park JA,Schluter SF,Marchalonis JJ,Akporiaye ET

    更新日期:2000-05-01 00:00:00

  • Roles of Ets proteins, NF-kappa B and nocodazole in regulating induction of transcription of mouse germline Ig alpha RNA by transforming growth factor-beta 1.

    abstract::Antibody class switch recombination (CSR) occurs after antigen activation of B cells. CSR is directed to specific heavy chain isotypes by cytokines and B cell activators that induce transcription from the unrearranged, or germline (GL), C(H) region genes. Transforming growth factor (TGF)-beta1 is essential for switch ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Shi MJ,Park SR,Kim PH,Stavnezer J

    更新日期:2001-06-01 00:00:00

  • Abrogation of CCL21 chemokine function by transgenic over-expression impairs T cell immunity to local infections.

    abstract::The CC chemokine receptor 7 (CCR7) and its two ligands, CCL21 and CCL19, play an important role in migration of immune cells to lymphoid tissue. To analyze the function of CCR7 in T cell immunity to infectious agents in vivo, transgenic (tg) mice expressing CCL21 in an ubiquitous fashion were generated. These mice con...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Unsoeld H,Mueller K,Schleicher U,Bogdan C,Zwirner J,Voehringer D,Pircher H

    更新日期:2007-11-01 00:00:00

  • IFN{alpha} enhances human B-cell chemotaxis by modulating ligand-induced chemokine receptor signaling and internalization.

    abstract::In this study, we show that IFNalpha increases the chemotaxis of human B cells to CCL20, CCL21 and CXCL12 in a dose- and time-dependent manner. The effect was maximal with 2000 IU ml(-1) IFNalpha. It peaked at 24 h and decreased thereafter. At 24 h, IFNalpha had increased B-cell chemotaxis to CCL20 by 20 +/- 6.2% (n =...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Badr G,Borhis G,Treton D,Richard Y

    更新日期:2005-04-01 00:00:00

  • PI3K is a negative regulator of IgE production.

    abstract::The production of IgE, a main player in allergic disorders such as asthma and atopic dermatitis, is strictly regulated and the serum concentrations of IgE are normally kept at a much lower level than other isotypes. We found that mice deficient for the p85alpha regulatory subunit of class IA phosphoinositide 3-kinase ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Doi T,Obayashi K,Kadowaki T,Fujii H,Koyasu S

    更新日期:2008-04-01 00:00:00

  • Generation of induced pluripotent stem cell-derived mice by reprogramming of a mature NKT cell.

    abstract::NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor α chain encoded by Vα14Jα18 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analys...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ren Y,Dashtsoodol N,Watarai H,Koseki H,Quan C,Taniguchi M

    更新日期:2014-10-01 00:00:00

  • Direct binding of IL-12 to human and murine B lymphocytes.

    abstract::IL-12 has been shown to play a central role in cell-mediated inflammatory reactions through direct activation of T cells and NK cells. IL-12 also strongly influences humoral immunity but these effects have been thought to be indirect and caused by intermediary cytokines. Using flow cytometry, we now show that IL-12 di...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Vogel LA,Showe LC,Lester TL,McNutt RM,Van Cleave VH,Metzger DW

    更新日期:1996-12-01 00:00:00

  • Characterization of an active, non-myristylated, cytoplasmic form of the lymphoid protein tyrosine kinase pp56lck.

    abstract::pp56lck is a member of the src family of tyrosine kinases mainly expressed in T lymphocytes. Src tyrosine kinases have been implicated in the control of cell growth and differentiation in different cell types, but the mechanism of regulation of these enzymes is poorly understood. In order to characterize the distinct ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Carrera AC,Li P,Roberts TM

    更新日期:1991-07-01 00:00:00

  • A novel co-stimulatory T cell antigen co-expressed on renal cell carcinoma.

    abstract::The A6H mAb raised primarily against human renal cell carcinoma (RCC) has previously been shown to bind strongly to RCC, to some degree to colon carcinoma but only marginally to a variety of normal tissues. Immunohistochemical analysis or RCC tissues containing tumor-infiltrating lymphocytes revealed that A6H stained ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Labuda T,Lando P,Björkdahl O,Kalland T,Vessella R,Hedlund G,Eriksson H,Sjögren HO,Dohlsten M

    更新日期:1995-09-01 00:00:00

  • Production of citrulline and ornithine by interferon-gamma treated macrophages.

    abstract::Activated macrophages exert strong arginase (ASE) activity that converts L-arginine into ornithine, the key precursor for putrescine and polyamine biosynthesis. Macrophages were previously also shown to generate nitric oxide that is derived from the guanido group of arginine by the oxidative deiminase (OAD) reaction. ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Benninghoff B,Lehmann V,Eck HP,Dröge W

    更新日期:1991-05-01 00:00:00

  • IL-7 transgenic mice: analysis of the role of IL-7 in the differentiation of thymocytes in vivo and in vitro.

    abstract::We have generated a high copy number transgenic mouse line in which expression of mouse IL-7 cDNA is under the control of the mouse MHC class II E alpha promoter. These mice were generated in order to see if IL-7 over-production in the thymus altered either thymocyte differentiation or the process of negative selectio...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Mertsching E,Burdet C,Ceredig R

    更新日期:1995-03-01 00:00:00

  • Vaccination of the Leishmania major susceptible BALB/c mouse. I. The precise selection of peptide determinant influences CD4+ T cell subset expression.

    abstract::BALB/c mice are susceptible to cutaneous leishmaniasis upon infection with Leishmania major while C57BL/6 are not. There is a major promastigote surface protease (PSP or gp63) which is available in both native and recombinant forms, and for which the primary amino acid sequence is known. Immunization with PSP has been...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Soares LR,Sercarz EE,Miller A

    更新日期:1994-05-01 00:00:00

  • Staphylococcal enterotoxin B-induced activation and concomitant resistance to cell death in CD28-deficient HLA-DQ8 transgenic mice.

    abstract::HLA class II molecules present superantigens more efficiently than their murine counterpart. Therefore, transgenic mice expressing HLA-DQ8 with and without CD28 were used to address the role of CD28 in staphylococcal enterotoxin B (SEB)-driven immune responses. SEB-induced in vitro proliferation of naive DQ8.CD28(-/-)...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Rajagopalan G,Smart MK,Marietta EV,David CS

    更新日期:2002-07-01 00:00:00

  • A thymic epithelial cell line induces both positive and negative selection in the thymus.

    abstract::TCR engagement in the thymus results in both survival and elimination signals for developing thymocytes. To examine whether both signals can be provided by the same cell type, we investigated the ability of a thymic epithelial cell (TEC) line 427.1, previously shown to allow positive selection in the thymus, to induce...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Vukmanović S,Jameson SC,Bevan MJ

    更新日期:1994-02-01 00:00:00

  • Maintenance of clonal anergy by endogenously produced IL-10.

    abstract::Inadequate co-stimulation of tumor reactive T cells may contribute to the fact that antigenic tumors are not normally rejected by the immune system. We recently reported that the induction of profound unresponsiveness in a T cell clone by melanoma cells expressing MHC class II antigens may provide a mechanism for thes...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Becker JC,Czerny C,Bröcker EB

    更新日期:1994-10-01 00:00:00

  • IL-17 and IL-22 enhance skin inflammation by stimulating the secretion of IL-1β by keratinocytes via the ROS-NLRP3-caspase-1 pathway.

    abstract:BACKGROUND:The pathogenesis of inflammatory skin disease involves the release of cytokines from keratinocytes, and one of these, IL-1β, has been previously implicated in inflammatory skin disease. T(h)17 cells, a subset of T(h) cells involved in autoimmunity and inflammation, possess IL-1β receptors and secrete cytokin...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cho KA,Suh JW,Lee KH,Kang JL,Woo SY

    更新日期:2012-03-01 00:00:00

  • Peripheral blood CD4+CD8+ lymphocytes in cynomolgus monkeys are of resting memory T lineage.

    abstract::In this study, we analyzed peripheral blood CD4+CD8+ double-positive (DP) lymphocytes in adult cynomolgus monkeys (Macaca fascicularis). Forty of 55 monkeys had > 5% of the peripheral blood DP subpopulation (9.3 +/- 5.9%; mean +/- SD) in peripheral blood lymphocytes (PBL) in contrast to a low percentage of peripheral ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Akari H,Terao K,Murayama Y,Nam KH,Yoshikawa Y

    更新日期:1997-04-01 00:00:00

  • Sulfates are main targets of immune responses to cruzipain and are involved in heart damage in BALB/c immunized mice.

    abstract::Trypanosoma cruzi, the agent of Chagas disease contains a major cysteine proteinase, cruzipain (Cz), with an unusual carboxyl-terminal extension (C-T). We have previously reported the presence of sulfate groups in the N-linked oligosaccharide chains of this domain. In order to evaluate the immune responses to sulfated...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Acosta DM,Arnaiz MR,Esteva MI,Barboza M,Stivale D,Orlando UD,Torres S,Laucella SA,Couto AS,Duschak VG

    更新日期:2008-04-01 00:00:00

  • Gene expression profiles during human CD4+ T cell differentiation.

    abstract::To develop a comprehensive catalogue of phenotypic and functional parameters of human CD4(+) T cell differentiation stages, we have performed microarray gene expression profiling on subpopulations of human thymocytes and circulating naive CD4(+) T cells, including CD3(-)CD4(+)CD8(-) intrathymic T progenitor cells, CD3...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lee MS,Hanspers K,Barker CS,Korn AP,McCune JM

    更新日期:2004-08-01 00:00:00

  • Characterization of the developmental landscape of murine RORγt+ iNKT cells.

    abstract::Invariant natural killer T (iNKT) cells expressing the retinoic acid receptor-related orphan receptor γt (RORγt) and producing IL-17 represent a minor subset of CD1d-restricted iNKT cells (iNKT17) in C57BL/6J (B6) mice. We aimed in this study to define the reasons for their low distribution and the sequence of events ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Klibi J,Li S,Amable L,Joseph C,Brunet S,Delord M,Parietti V,Jaubert J,Marie J,Karray S,Eberl G,Lucas B,Toubert A,Benlagha K

    更新日期:2020-02-07 00:00:00

  • Arid5a-deficient mice are highly resistant to bleomycin-induced lung injury.

    abstract::Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are among the major causes of death worldwide due to acute inflammation in the lung. AT-rich interactive domain-containing 5a (Arid5a) is an RNA-binding protein involved in inflammatory autoimmune disease through post-transcriptional control of Il6...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Dubey PK,Masuda K,Nyati KK,-Uz Zaman MM,Chalise JP,Millrine D,Kai W,Ripley B,Kishimoto T

    更新日期:2017-02-01 00:00:00

  • IL-3 facilitates lymphocyte hexose transport by enhancing the intrinsic activity of the transport system.

    abstract::Interleukin 3 (IL-3) promotes the survival and proliferation of hematopoietic cells of various lineages in culture. Like most other hematopoietic colony stimulating factors, its mode of action is unknown. However, binding of the lymphokine induces protein tyrosine phosphorylation and enhanced glucose transport in some...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nefesh I,Bauskin AR,Alkalay I,Golembo M,Ben-Neriah Y

    更新日期:1991-08-01 00:00:00

  • Major population differences in T cell response to a malaria sporozoite vaccine candidate.

    abstract::Using a complete series of overlapping peptides, we have identified the T cell epitopes of a malaria vaccine candidate, the circumsporozoite (CS) protein, that are recognized by sporozoite-exposed residents of a non-endemic country. This protein and subunits from it are being considered as malaria sporozoite vaccine c...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Zevering Y,Houghten RA,Frazer IH,Good MF

    更新日期:1990-01-01 00:00:00

  • HLA-G functions as a restriction element and a transplantation antigen in mice.

    abstract::HLA-G, a human MHC class I molecule expressed on the trophoblast during pregnancy, was expressed in transgenic mice by recombining the HLA-G gene with a transcriptional promoter from a murine H-2 MHC class I gene. Skin grafts from HLA-G transgenic mice were rejected by non-transgenic mice showing that HLA-G behaves as...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Horuzsko A,Antoniou J,Tomlinson P,Portik-Dobos V,Mellor AL

    更新日期:1997-05-01 00:00:00

  • IL-2 production by myofibroblasts from post-radiation fibrosis in breast cancer patients.

    abstract::The origin of cell activation in post-radiation fibrosis and its chronic extension are still poorly understood. Since local IL-2 cancer treatment sometimes triggers intraperitoneal fibrosis we have analyzed three myofibroblastic cell strains from post-radiation skin fibrosis (FPR7, FPR10 and FPR15) for their interacti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Alileche A,Han D,Plaisance S,Assier E,Sahraoui Y,Clemanceau C,Metivier D,Brouty-Boyer D,Jasmin C,Azzarone B

    更新日期:1994-10-01 00:00:00