IL-7 transgenic mice: analysis of the role of IL-7 in the differentiation of thymocytes in vivo and in vitro.

Abstract:

:We have generated a high copy number transgenic mouse line in which expression of mouse IL-7 cDNA is under the control of the mouse MHC class II E alpha promoter. These mice were generated in order to see if IL-7 over-production in the thymus altered either thymocyte differentiation or the process of negative selection. Using in situ hybridization, IL-7 transcripts could be detected in the thymic cortex and medulla as well as the spleen and lymph nodes of transgenic mice but was undetectable in normal controls. Phenotypic and molecular analysis of thymocytes from embryonic and adult transgenic mice failed to reveal a dramatic effect of IL-7 on thymocyte differentiation and negative selection of the TCR V beta repertoire appeared to be intact. In peripheral lymph nodes, there was a massive (30-fold) increase in the number of T cells (CD8+ > CD4+) and simultaneous presence of immature (B220+, Ig-) B cells. TCR repertoire analysis showed that the expansion of peripheral T cells was polyclonal. Using the polymerase chain reaction (PCR), transgene-specific IL-7 transcripts could be detected in the thymus from day 14 of fetal development. However, using semi-quantitative PCR, there was no dramatic increase in the degree of TCR beta or TCR alpha gene rearrangements during thymocyte ontogeny in vivo. Similarly, when fetal mouse thymus lobes were cultured with IL-7 in vitro, there was no dramatic increase in the degree of TCR beta or TCR alpha gene rearrangements. We conclude that IL-7 is probably not an important differentiation factor for immature mouse thymocytes.

journal_name

Int Immunol

journal_title

International immunology

authors

Mertsching E,Burdet C,Ceredig R

doi

10.1093/intimm/7.3.401

subject

Has Abstract

pub_date

1995-03-01 00:00:00

pages

401-14

issue

3

eissn

0953-8178

issn

1460-2377

journal_volume

7

pub_type

杂志文章
  • The expression of B cell surface receptors. III. The murine low-affinity IgE Fc receptor is not expressed on Ly 1 or 'Ly 1-like' B cells.

    abstract::The distribution of IgE FcR (Fc epsilon R)-positive and -negative B cells was examined in normal adult mice. Using three-color flow cytometry, the expression of the Fc epsilon R was analyzed on various B-cell subsets present in the peritoneum and spleen. The results demonstrate that in the peritoneal cavity, the Fc ep...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/3.4.305

    authors: Waldschmidt TJ,Kroese FG,Tygrett LT,Conrad DH,Lynch RG

    更新日期:1991-04-01 00:00:00

  • Immunopathogenesis of hepatic fibrosis in chronic liver injury induced by repeatedly administered concanavalin A.

    abstract::Liver fibrosis is commonly observed in chronic liver disease. However, the immunological mechanisms underlying hepatic fibrosis due to chronic inflammation are not well defined, mainly because suitable experimental models have not been established. We have found that weekly i.v. administration of concanavalin A (Con A...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/11.9.1491

    authors: Kimura K,Ando K,Ohnishi H,Ishikawa T,Kakumu S,Takemura M,Muto Y,Moriwaki H

    更新日期:1999-09-01 00:00:00

  • Transendothelial migration of effector T cells across inflamed endothelial barriers does not require heparan sulfate proteoglycans.

    abstract::Leukocyte diapedesis is a chemotactic multistep process that requires optimal chemoattractant presentation by the endothelial barrier. Recent studies have described a critical role for heparan sulfate glycosaminoglycans (HSGAGs) in the presentation and functions of chemokines essential for lymphocyte interactions with...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxt076

    authors: Stoler-Barak L,Barzilai S,Zauberman A,Alon R

    更新日期:2014-06-01 00:00:00

  • Anti-CD3 induces bi-phasic apoptosis in murine intestinal epithelial cells: possible involvement of the Fas/Fas ligand system in different T cell compartments.

    abstract::Recent studies have suggested that Fas-mediated apoptosis is involved in the pathogenesis of intestinal injury. In this study, we determined the role of Fas/Fas ligand (FasL) interactions in different T cell compartments using a murine model of small intestinal injury. An intraperitoneal injection of 145-2C11 (anti-CD...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxh231

    authors: Miura N,Yamamoto M,Fukutake M,Ohtake N,Iizuka S,Ishige A,Sasaki H,Fukuda K,Yamamoto T,Hayakawa S

    更新日期:2005-05-01 00:00:00

  • Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-alpha.

    abstract::We demonstrate here that human necrosis factor-alpha, a potent neutrophil activator, induces rapid (within 3 h) apoptosis of these cells, i.e. neutrophils treated with this cytokine exhibit (i) light and electron microscopic changes characteristic to apoptotic cells, (ii) reduced propidium iodide binding to DNA, and (...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/5.6.691

    authors: Takeda Y,Watanabe H,Yonehara S,Yamashita T,Saito S,Sendo F

    更新日期:1993-06-01 00:00:00

  • HIV envelope-directed signaling aberrancies and cell death of CD4+ T cells in the absence of TCR co-stimulation.

    abstract::HIV-1 infection in CD4(+) T cells initiates a viral cytopathic effect (CPE) that is dependent on the activation of intracellular protein tyrosine kinases (PTK). PTK in T cells are also activated during the course of TCR or CD4 receptor engagement and the manner of receptor engagement may generate signals leading eithe...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.1.65

    authors: Tian H,Lempicki R,King L,Donoghue E,Samelson LE,Cohen DI

    更新日期:1996-01-01 00:00:00

  • Molecular mimicry: anti-DNA antibodies may arise inadvertently as a response to antibodies generated to microorganisms.

    abstract::The origin of anti-DNA antibodies remains speculative. We argue that some of these antibodies may arise inadvertently in nature during the course of a normal immune response due to their induction by antibodies which bear structures (mimotopes) that mimic DNA. These antibodies are not necessarily DNA specific but, lik...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/13.9.1099

    authors: Wun HL,Leung DT,Wong KC,Chui YL,Lim PL

    更新日期:2001-09-01 00:00:00

  • Use of synthetic peptides to probe lymphocyte--high endothelial cell interactions. Lymphocytes recognize a ligand on the endothelial surface which contains the CS1 adhesion motif.

    abstract::The extravasation of recirculating lymphocytes into lymph nodes, which is crucial for immune system function, occurs constitutively from specialized post-capillary venules in the lymph node paracortex. The migration of lymphocytes between the structurally distinct high endothelial cells which line these blood vessels ...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/2.10.921

    authors: Ager A,Humphries MJ

    更新日期:1990-01-01 00:00:00

  • Requirement of high-affinity IL-2-IL-2R interaction for T cell anergy induction.

    abstract::Incomplete T cell antigen receptor-mediated signaling induces an unresponsive state known as anergy. Previously, we had shown that anergy can be induced in antigen-primed but not naive T cells. In this report, we found that in vitro primed T cells from IL-2R alpha-deficient mice were resistant to anergy induction in c...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxh397

    authors: Hayashi RJ,Kanagawa O

    更新日期:2006-05-01 00:00:00

  • mAb against hen egg-white lysozyme regulate its presentation to CD4(+) T cells.

    abstract::Specific antibodies increase antigen uptake and presentation by antigen-presenting cells via the B cell receptor in B cells or FcgammaR in dendritic cells. To determine whether the interaction between antibody and antigen could influence the set of peptides presented by MHC II molecules, we analyzed the presentation o...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/11.11.1863

    authors: Guermonprez P,Lo-Man R,Sedlik C,Rojas MJ,Poljak RJ,Leclerc C

    更新日期:1999-11-01 00:00:00

  • Selective recognition of malaria antigens by human serum antibodies is not genetically determined but demonstrates some features of clonal imprinting.

    abstract::Malaria infection induces the production of serum antibodies to a variety of malaria antigens but the prevalence of antibodies to any particular antigen is typically much less than 100%. It has been assumed that non-responsiveness to defined antigens in malaria immune subjects is due to HLS-mediated restriction of the...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.6.905

    authors: Taylor RR,Egan A,McGuinness D,Jepson A,Adair R,Drakely C,Riley E

    更新日期:1996-06-01 00:00:00

  • Immune regulatory effects of central nervous system antigens in culture.

    abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/12.11.1605

    authors: Lindsey JW,Jin R

    更新日期:2000-11-01 00:00:00

  • Immunofluorescence analysis of B-1 cell ontogeny in the mouse.

    abstract::In order to further understand the developmental aspects of B-1 cells, we characterized the ontogeny of this B cell population in the spleen and peritoneal cavity of BALB/c mice. Although there are B-1 cells in the spleen within the first 1-3 weeks after birth, they do not at any stage represent the majority of spleni...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/6.3.355

    authors: Hamilton AM,Lehuen A,Kearney JF

    更新日期:1994-03-01 00:00:00

  • How to master the host immune system? Leishmania parasites have the solutions!

    abstract::Infection by protozoan parasites of the genus Leishmania results in the development of leishmaniasis, an increasingly prevalent group of diseases affecting over 12 million people worldwide. Leishmaniasis can have very different outcomes ranging from cutaneous lesions, mucosal lesions to visceralization depending on th...

    journal_title:International immunology

    pub_type: 杂志文章,评审

    doi:10.1093/intimm/dxx075

    authors: Rossi M,Fasel N

    更新日期:2018-03-10 00:00:00

  • Interleukin 6 in inflammation, autoimmunity and cancer.

    abstract::Interleukin 6 (IL-6) is involved both in immune responses and in inflammation, hematopoiesis, bone metabolism and embryonic development. IL-6 plays roles in chronic inflammation (closely related to chronic inflammatory diseases, autoimmune diseases and cancer) and even in the cytokine storm of corona virus disease 201...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxaa078

    authors: Hirano T

    更新日期:2020-12-18 00:00:00

  • Production of IFN-γ by CD4(+) T cells in response to malaria antigens is IL-2 dependent.

    abstract::T-cell immune responses are critical for protection of the host and for disease pathogenesis during infection with Plasmodium species. We examined the regulation of CD4(+) T-cell cytokine responses during infection with Plasmodium berghei ANKA (PbA). CD4(+) T cells from PbA-infected mice produced IFN-γ, IL-4 and IL-10...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxq448

    authors: Kimura D,Miyakoda M,Honma K,Shibata Y,Yuda M,Chinzei Y,Yui K

    更新日期:2010-12-01 00:00:00

  • Elevated expression levels of an Ig transgene in mice links the IgH 3' enhancer to the regulation of IgH expression.

    abstract::To delineate the role of the IgH 3' enhancer in the regulation of Ig heavy (IgH) chain gene expression, mice harbouring rearranged IgH transgenes, with (PSVmu3) and without (PSVmu1) this element, were produced. RNA and protein analysis from the different transgenic lines revealed a 5- to 7-fold increase in the express...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.7.1149

    authors: Arulampalam V,Furebring C,Samuelsson A,Lendahl U,Borrebaeck C,Lundkvist I,Pettersson S

    更新日期:1996-07-01 00:00:00

  • Osteoclasts support the survival of human plasma cells in vitro.

    abstract::The aim of this in vitro study was to evaluate if osteoclasts (OCs) and dendritic cells (DCs), both of monocyte origin, can support the survival of normal human plasma cells (PCs). PCs differentiate from plasmablasts (PBs) arising from activated B cells, essentially memory B cells. To study the survival of both PBs (C...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxn035

    authors: Geffroy-Luseau A,Jégo G,Bataille R,Campion L,Pellat-Deceunynck C

    更新日期:2008-06-01 00:00:00

  • IL-7 reverses NK1+ T cell-defective IL-4 production in the non-obese diabetic mouse.

    abstract::Converging data suggest an important role for IL-7 in T lymphocyte maturation as illustrated by the severe T lymphopenia observed in IL-7-deficient mice. We recently reported that IL-7 preferentially promotes the in vitro expansion of a discrete MHC class I-dependent lymphocyte subset comprising both CD4+ and CD4-CD8-...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.11.1751

    authors: Gombert JM,Tancrède-Bohin E,Hameg A,Leite-de-Moraes MC,Vicari A,Bach JF,Herbelin A

    更新日期:1996-11-01 00:00:00

  • Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11.

    abstract::Quantitative expression of class I genes varies widely in different tissues, during development and in some neoplastic cells. Regulatory DNA sequences controlling levels of transcription of class I genes have been characterized in the murine and swine promoters. Although some regulatory features appear to be retained ...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/6.10.1485

    authors: Blanchet O,Gazin C,L'Haridon M,Tatari Z,Degos L,Sigaux F,Paul P

    更新日期:1994-10-01 00:00:00

  • Molecular cloning and characterization of guinea pig Fc gamma RIII: expression but not function is independent of the gamma chain of Fc epsilon RI.

    abstract::We have isolated two cDNA clones encoding the guinea pig receptor for the Fc portion of IgG2 (Fc gamma 2R) from a guinea pig peritoneal macrophage cDNA library. Analysis of the predicted amino acid sequence of the one cDNA clone indicated that the guinea pig Fc gamma 2R is a type I transmembrane protein and has approx...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.9.1335

    authors: Isashi Y,Yamashita T,Nagasawa S,Murakami M,Uede T

    更新日期:1996-09-01 00:00:00

  • Conserved regions of protein disulfide isomerase are targeted by natural IgA antibodies in humans.

    abstract::Secretory IgA (sIgA) antibodies in human tears and milk were found to recognize protein disulfide isomerase (PDI) on a Toxoplasma gondii lysate immunoblot (IB). These antibodies were already detectable in tears of infants. To determine the epitope containing-regions on PDI, we generated truncated versions of recombina...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxf091

    authors: Meek B,Back JW,Klaren VN,Speijer D,Peek R

    更新日期:2002-11-01 00:00:00

  • The TATA binding protein, c-Myc and survivin genes are not somatically hypermutated, while Ig and BCL6 genes are hypermutated in human memory B cells.

    abstract::Immunoglobulin (IG:) genes are hypermutated in mature B cells after interaction with antigen and T cells in a germinal center reaction. We and others have recently shown that the human BCL6 gene is also hypermutated in human peripheral blood memory B cells and tonsils. A preliminary analysis of other non-Ig genes (c-M...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/12.7.1085

    authors: Shen HM,Michael N,Kim N,Storb U

    更新日期:2000-07-01 00:00:00

  • Roles of Ets proteins, NF-kappa B and nocodazole in regulating induction of transcription of mouse germline Ig alpha RNA by transforming growth factor-beta 1.

    abstract::Antibody class switch recombination (CSR) occurs after antigen activation of B cells. CSR is directed to specific heavy chain isotypes by cytokines and B cell activators that induce transcription from the unrearranged, or germline (GL), C(H) region genes. Transforming growth factor (TGF)-beta1 is essential for switch ...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/13.6.733

    authors: Shi MJ,Park SR,Kim PH,Stavnezer J

    更新日期:2001-06-01 00:00:00

  • T cell response of I-Aq mice to self type II collagen: meshing of the binding motif of the I-Aq molecule with repetitive sequences results in autoreactivity to multiple epitopes.

    abstract::Type II collagen (CII) is of immunological interest because of its repetitive structure and properties as an autoantigen. The mouse gene has recently been cloned, thus enabling T cell-defined epitopes to be identified. Multiple novel epitopes on mouse CII are here detected in the autoreactive T cell response. The majo...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/9.11.1687

    authors: Bayrak S,Holmdahl R,Travers P,Lauster R,Hesse M,Dölling R,Mitchison NA

    更新日期:1997-11-01 00:00:00

  • IL-3 facilitates lymphocyte hexose transport by enhancing the intrinsic activity of the transport system.

    abstract::Interleukin 3 (IL-3) promotes the survival and proliferation of hematopoietic cells of various lineages in culture. Like most other hematopoietic colony stimulating factors, its mode of action is unknown. However, binding of the lymphokine induces protein tyrosine phosphorylation and enhanced glucose transport in some...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/3.8.827

    authors: Nefesh I,Bauskin AR,Alkalay I,Golembo M,Ben-Neriah Y

    更新日期:1991-08-01 00:00:00

  • A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice.

    abstract::The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneo...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxh143

    authors: Takasuka N,Fujii H,Takahashi Y,Kasai M,Morikawa S,Itamura S,Ishii K,Sakaguchi M,Ohnishi K,Ohshima M,Hashimoto S,Odagiri T,Tashiro M,Yoshikura H,Takemori T,Tsunetsugu-Yokota Y

    更新日期:2004-10-01 00:00:00

  • Differential antibody binding to the surface alphabetaTCR.CD3 complex of CD4+ and CD8+ T lymphocytes is conserved in mammals and associated with differential glycosylation.

    abstract::We have previously shown that the surface alphabeta T cell antigen receptor (TCR).CD3 complex borne by human CD4(+) and CD8(+) T lymphocytes can be distinguished using mAbs. Using two unrelated sets of antibodies, we have now extended this finding to the surface alphabetaTCR.CD3 of seven additional mammalian species (...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxn081

    authors: Rossi NE,Reiné J,Pineda-Lezamit M,Pulgar M,Meza NW,Swamy M,Risueno R,Schamel WW,Bonay P,Fernández-Malavé E,Regueiro JR

    更新日期:2008-10-01 00:00:00

  • IL-17 and IL-22 enhance skin inflammation by stimulating the secretion of IL-1β by keratinocytes via the ROS-NLRP3-caspase-1 pathway.

    abstract:BACKGROUND:The pathogenesis of inflammatory skin disease involves the release of cytokines from keratinocytes, and one of these, IL-1β, has been previously implicated in inflammatory skin disease. T(h)17 cells, a subset of T(h) cells involved in autoimmunity and inflammation, possess IL-1β receptors and secrete cytokin...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/dxr110

    authors: Cho KA,Suh JW,Lee KH,Kang JL,Woo SY

    更新日期:2012-03-01 00:00:00

  • HLA-DM and MHC class II molecules co-distribute with peptidase-containing lysosomal subcompartments.

    abstract::MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HL...

    journal_title:International immunology

    pub_type: 杂志文章

    doi:10.1093/intimm/8.5.625

    authors: Fernandez-Borja M,Verwoerd D,Sanderson F,Aerts H,Trowsdale J,Tulp A,Neefjes J

    更新日期:1996-05-01 00:00:00