Abstract:
:Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germline band. The same band was also observed in analysis with a 5' DQ probe, a 5'-flanking sequence of DQ52, thus suggesting the use of DQ52. By analyzing EcoRI- and PvuII-digested genomic DNA, the above-observed band was demonstrated to be the result of DQ52-JH2 joining. This was further confirmed utilizing a T cell leukemia line with known DQ52-JH joinings. We then quantitatively estimated the frequencies of JH segment usages in joining with DQ52, through plaque hybridization assays. Three hundred and fifty JH4-positive clones were obtained from 7 x 10(5) plaques by plaque hybridization. Forty-eight randomly selected non-germline clones were purified and the use of JH segments was determined through Southern blot analysis. Nine clones used JH1, 29 used JH2, 9 used JH3, and 1 used JH4, thus indicating an apparent preferential usage of JH2 segment. Southern blot analysis of genomic DNA of progenitor B cells in culture showed a dominant band of DQ52-JH2 joining 1 week after initiation of culture. This may indicate that the dominant DQ52-JH2 joining in thymocytes is representative of early D-J joinings in progenitor B cells.
journal_name
Int Immunoljournal_title
International immunologyauthors
Suzuki H,Abe M,Nishikawa S,Nakayama E,Shiku Hdoi
10.1093/intimm/1.6.643subject
Has Abstractpub_date
1989-01-01 00:00:00pages
643-6issue
6eissn
0953-8178issn
1460-2377journal_volume
1pub_type
杂志文章abstract::Dendritic cells (DCs) are specialized antigen-presenting cells that play pivotal roles in initiating immune responses. However, DC maturation is usually strongly restricted by the stromal microenvironment, especially in non-lymphoid tissues, such as skin and mucosa. Although suppression of DC maturation by stromal cel...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn078
更新日期:2008-09-01 00:00:00
abstract::Protein antigens elicit humoral responses in mice that consist predominantly of IgG1 antibodies. We have now investigated the ability of IL-12, a cytokine reported to augment IgG2a anti-hapten responses through activation of Th1 cells, to alter antibody responses to hen eggwhite lysozyme (HEL). The normal response of ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.9.1519
更新日期:1995-09-01 00:00:00
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journal_title:International immunology
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.12.1489
更新日期:2001-12-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) signaling pathway is strongly activated in response to TCR stimulation in normal T cells. However, the extent to which activation of the ERK pathway is necessary for TCR-stimulated cytokine production is not clear. We have addressed this question by use of two separate m...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.2.223
更新日期:1998-02-01 00:00:00
abstract::Experiments in transgenic mice have demonstrated that thymocyte differentiation into the mature CD4+ helper or CD8+ cytotoxic T cell lineage is ultimately dependent upon the specificity of the TCR for class II or class I MHC molecules respectively. However, the initial mechanistic events involved in this process remai...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.4.541
更新日期:1995-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt061
更新日期:2014-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh381
更新日期:2006-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg006
更新日期:2003-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg073
更新日期:2003-06-01 00:00:00
abstract::Recent studies have revealed a role for basophils as antigen-presenting cells that preferentially induce T(h)2 cells in response to complexes of antigen plus antigen-specific IgE, to protease allergens or to helminthic parasites in vitro and in vivo through the production of 'early IL-4' and the presentation to CD4(+)...
journal_title:International immunology
pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn024
更新日期:2008-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl100
更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/3.8.827
更新日期:1991-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.7.821
更新日期:1992-07-01 00:00:00
abstract::Tyrosine kinase-2 (Tyk2) participates in the signaling pathways of multiple cytokines in innate and acquired immunity. In the present study, we investigated the in vivo involvement of Tyk2 in anti-type II collagen antibody-induced arthritis (CAIA) using Tyk2-deficient mice. Hind paws of wild-type mice showed massive s...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxr057
更新日期:2011-09-01 00:00:00
abstract::Naturally occurring CD4(+)CD25(+) regulatory T cells (nT(R)) comprise a separate lineage of T cells that are essential for maintaining immunological tolerance to self. Here we demonstrate that the level of phosphorylation of the TCR zeta-chain is approximately 1.5- to 4-fold higher in nT(R) as compared with CD4(+)CD25...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm021
更新日期:2007-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz082
更新日期:2020-05-08 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.5.693
更新日期:1994-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
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更新日期:2018-08-30 00:00:00
abstract::NK cells were first identified in 1975 and represent the prototypical group 1 innate lymphoid cell (ILC). More recently, the discovery of new members of the ILC family has highlighted the complexity of this innate lymphoid lineage. Importantly, it has been recognized that different subsets exist within the group 1 ILC...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxv051
更新日期:2016-01-01 00:00:00
abstract::The thymus leukemia antigens (TL) belong to the MHC class Ib family and can be recognized by CD8-dependent or -independent cytotoxic T lymphocytes (CTL) showing TL, but not H-2, restriction. We previously reported that the CTL epitope is TAP independent and in the present study we further characterize the recognition ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg131
更新日期:2003-11-01 00:00:00
abstract::The TCR is responsible for the specificity of cytotoxic T lymphocytes (CTL) by recognizing peptides presented in the context of MHC. By producing recombinant soluble TCR, it is possible to study this interaction at the molecular level. We generated single-chain TCR (scTCR) from tumor infiltrating lymphocytes (TIL) and...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.5.745
更新日期:1999-05-01 00:00:00
abstract::Cyclosporin A (CsA) prevents most immature thymocytes from progressing to a mature phenotype and blocks the deletion of T cells that express self-specific TCR in the small population of cells that achieve maturity. The latter effect may explain the paradoxical observation that this immunosuppressive drug can induce au...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.12.1341
更新日期:1992-12-01 00:00:00
abstract::The profile of cytokines produced by CD4 T cells is profoundly influenced by the presence of IL-12. Here we demonstrate that during re-stimulation of antigen-specific immune responses in vitro, antigen-primed lymph node cells from DBA/2 mice produced 3- to 30-fold more IL-12 than did cells from BALB/c mice, which are ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.10.1511
更新日期:1996-10-01 00:00:00
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journal_title:International immunology
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更新日期:2006-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.1.21
更新日期:1999-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz033
更新日期:2019-10-16 00:00:00
abstract::The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneo...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh143
更新日期:2004-10-01 00:00:00