Abstract:
:Quantitative expression of class I genes varies widely in different tissues, during development and in some neoplastic cells. Regulatory DNA sequences controlling levels of transcription of class I genes have been characterized in the murine and swine promoters. Although some regulatory features appear to be retained in humans, most of them are not evolutionary conserved. In this study, we identify novel DNA sequences and factors which contribute to the regulation of the human HLA-A11 gene. Two activator elements (-155 to -91 and -335 to -206) and one negative element (-172 to -156), distinct from those previously described are mapped within 335 bp upstream of exon 1 of the human HLA-A11 gene. Various nuclear factors bind to these regulatory elements, some of which are cell restricted or interact with evolutionary divergent regulatory sequences of the human class I gene promoter. Two of the five DNA-binding sites characterized in this work bind at least two proteins which compete for the occupancy of their site. The identification of these new regulatory elements provides a more comprehensive basis for the understanding of physiological or pathological modulation of MHC class I transcription in humans.
journal_name
Int Immunoljournal_title
International immunologyauthors
Blanchet O,Gazin C,L'Haridon M,Tatari Z,Degos L,Sigaux F,Paul Pdoi
10.1093/intimm/6.10.1485subject
Has Abstractpub_date
1994-10-01 00:00:00pages
1485-96issue
10eissn
0953-8178issn
1460-2377journal_volume
6pub_type
杂志文章abstract::Eye-derived antigen-presenting cells (APCs) are known to contribute to the immune privilege status of the eye by inducing a form of peripheral tolerance that deviates T(h)1 type of pro-inflammatory immune responses. Similar systemic tolerance can also be induced by non-ocular APCs exposed to transforming growth factor...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl006
更新日期:2006-05-01 00:00:00
abstract::The human IL-3 gene, located on chromosome 5, contains several cis-acting DNA sequences, i.e. CLE (conserved lymphokine element) and a GC-rich region, similar to the GM-CSF gene. To investigate the role of these elements, the 5' flanking region of the IL-3 gene was attached to a bacterial chloramphenicol acetyltransfe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.3.245
更新日期:1991-03-01 00:00:00
abstract::The Ig heavy chain (IgH) locus is controlled by multiple regulatory sequences mapping both within the IgH transcription unit (E mu) and downstream (3') of IgH coding sequences (hs3a, hs1,2, hs3b and hs4). Enhancer knockout studies in mice have implicated E mu in the control of IgH variable region gene assembly, but si...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.8.1003
更新日期:2001-08-01 00:00:00
abstract::Leukocyte diapedesis is a chemotactic multistep process that requires optimal chemoattractant presentation by the endothelial barrier. Recent studies have described a critical role for heparan sulfate glycosaminoglycans (HSGAGs) in the presentation and functions of chemokines essential for lymphocyte interactions with...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt076
更新日期:2014-06-01 00:00:00
abstract::Cross-linking of the IgM antigen receptor on an immature B cell lymphoma (BKS-2) induces growth arrest and apoptosis. This is accompanied by down-regulation of the immediate early genes, egr-1 and c-myc, and a reduction in NF-kappaB activity. Anti-IgM-induced growth arrest and apoptosis of this murine B cell lymphoma ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.6.871
更新日期:1999-06-01 00:00:00
abstract::1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] and the vitamin D receptor (VDR) are important regulators of autoimmunity. The effect of the VDR on the ability of mice to fight a primary or secondary infection has not been determined. Young and old VDR knockout (KO) mice were able to clear both primary and secondary infe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn129
更新日期:2009-02-01 00:00:00
abstract::'Programmed cell death' has been used to describe the death of cells killed by cytotoxic T cells or growth factor deprivation. Although bcl-2 can prevent death of cells deprived of growth factor, it failed to protect cells against T cell killing. In spite of bcl-2 expression, the DNA of targeted cells was degraded int...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.7.821
更新日期:1992-07-01 00:00:00
abstract::We have isolated two cDNA clones encoding the guinea pig receptor for the Fc portion of IgG2 (Fc gamma 2R) from a guinea pig peritoneal macrophage cDNA library. Analysis of the predicted amino acid sequence of the one cDNA clone indicated that the guinea pig Fc gamma 2R is a type I transmembrane protein and has approx...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.9.1335
更新日期:1996-09-01 00:00:00
abstract::Lymphocyte homing to lymph nodes is regulated by transient but specific interactions between lymphocytes and high endothelial venules (HEVs), the initial phase of which is mainly governed by the leukocyte adhesion molecule L-selectin, which recognizes sulfated and sialylated O-linked oligosaccharides displayed on sial...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh128
更新日期:2004-09-01 00:00:00
abstract::After activation, populations of antigen-specific T cells flow between sites of antigen expression, local lymphoid structures and other lymphoid and non-lymphoid organs. In this study, we documented the in vivo dynamics of a CD8(+) T cell response to antigen delivered using herpes simplex virus amplicon vectors and re...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm086
更新日期:2007-10-01 00:00:00
abstract::Neural stem cells (NSCs) transplantation has been proposed as a means of restoring damaged brain tissue, a possibility rendered more likely by reports of low NSCs immunogenicity in various experimental models because of low expression of MHC class I and II as well as co-stimulatory molecules. We investigated the immun...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm079
更新日期:2007-09-01 00:00:00
abstract::The granulocyte macrophage colony stimulating factor (GM-CSF) promoter contains a 10 bp element known as CK-1 or CD28RE that specifically responds to the co-stimulatory signal delivered to T cells via the CD28 surface receptor. This element is a variant NFkappaB site that does not function alone but requires an adjace...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.12.1945
更新日期:1999-12-01 00:00:00
abstract::The human chymase (HC) is a major granule constituent of mast cells (MCs) residing in the connective tissue and the sub-mucosa. Although many potential substrates have been described for this important MC enzyme, its full range of in vivo substrates has most likely not yet been identified. A major step toward a better...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn128
更新日期:2009-01-01 00:00:00
abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.11.1605
更新日期:2000-11-01 00:00:00
abstract::IL-18 is a proinflammatory cytokine that plays an important role in NK cell activation and T(h)1 response. IL-18 has a structural homology to IL-1, particularly IL-1beta. IL-18R, composed of IL-1R-related protein (IL-18Ralpha) and IL-1R accessory protein-like (IL-18Rbeta), belongs to the IL-1R family. Furthermore, IL-...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.2.151
更新日期:2000-02-01 00:00:00
abstract::The class II trans-activator (CIITA) is a bi- or multi-functional domain protein which plays a critical role in the expression of MHC class II genes. We report that removal of the N-terminal 151 amino acids, encompassing all of the acidic domain but leaving intact the proline/serine/threonine-rich domain, results in a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1545
更新日期:1997-10-01 00:00:00
abstract::Human intestinal intraepithelial lymphocytes (iIEL) are a unique population of predominantly CD8 alpha beta+, TCR alpha beta+ lymphocytes and, to a lesser extent, TCP gamma delta+ lymphocytes that proliferate poorly to anti-CD3 mitogenic signals but display significant cytolytic activity. Studies in mouse model system...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/7.8.1237
更新日期:1995-08-01 00:00:00
abstract::Hepatitis C virus (HCV) has the ability to persist in the majority of infected people. Strong, multispecific and sustained T-cell response is correlated with viral clearance. The mechanisms of chronicity by HCV are unclear. HCV could restrain the immune system and establish chronic infection by modulating dendritic ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq033
更新日期:2010-06-01 00:00:00
abstract::Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.7.691
更新日期:1991-07-01 00:00:00
abstract::Natural cytotoxicity receptors and NKG2D correspond to major activating receptors involved in triggering of tumor cell lysis by human NK cells. In this report, we investigated the expression of NKG2D ligands (NKG2DLs), MHC class I-related chain (MIC) A, MICB and UL16-binding proteins 1, 2 and 3, on a panel of human no...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn038
更新日期:2008-07-01 00:00:00
abstract::Spectroscopic methods have been applied to elucidate conformational differences responsible for the immunological diversity of two synthetic multichain copolymers, Tyr1Tyr2Glu3Glu4-poly-DL-Ala--poly-Lys and Tyr1Glu2Tyr3Glu4-poly-DL-Ala--poly-Lys. Despite their far-reaching structural similarity in the epitope peptide ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.591
更新日期:1993-06-01 00:00:00
abstract::The B cell-activating factor from the tumor necrosis factor family (BAFF) is an important regulator of B cell immunity. Recently, we demonstrated that recombinant BAFF also provides a co-stimulatory signal to T cells. Here, we studied expression of BAFF in peripheral blood leukocytes and correlated this expression wit...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh043
更新日期:2004-03-01 00:00:00
abstract::The CD2 co-receptor expressed on the surface of T lymphocytes is able to stimulate T cell activation, proliferation and cytokine production in the absence of direct engagement of the antigen-specific TCR. Engagement of human CD2 by mitogenic pairs of anti-CD2 mAb induces tyrosine phosphorylation of a number of intrace...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.1.13
更新日期:2001-01-01 00:00:00
abstract::Human dendritic cells (DC) generated from CD34+ hematopoietic progenitors cultured in the presence of granulocyte macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha are related to Langerhans cells (DLC) and have been shown to induce a strong proliferation of allogeneic CD4+ T cells. Th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1177
更新日期:1994-08-01 00:00:00
abstract::Naive CD4(+) T cells rapidly proliferate to generate effector cells after encountering an antigen and small numbers survive as memory T cells in preparation for future immunological events. In the present work, adoptive transfer of naive CD4(+) T cells into RAG2(-/-) mice caused the generation of memory-type effector ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm114
更新日期:2007-12-01 00:00:00
abstract::O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1409
更新日期:1994-09-01 00:00:00
abstract::Several cytokines and chemokines including chemokine (C-C motif) ligand-2 (CCL2) are induced in HIV-1 infection. However, the impact of HIV-1 viremia on CCL2 regulation is largely unknown. We utilized a DNA oligonucleotide microarray covering 110 inflammatory genes. Five genes were induced by at least 2-fold in PBMCs ...
journal_title:International immunology
pub_type: 临床试验,杂志文章
doi:10.1093/intimm/dxl078
更新日期:2006-10-01 00:00:00
abstract::The lectin complement pathway is initiated by binding of mannose-binding lectin (MBL) and MBL-associated serine protease (MASP) to carbohydrates. In the human lectin pathway, MASP-1 and MASP-2 are involved in the proteolysis of C4, C2 and C3. Here we report that the human MBL-MASP complex contains a new 22 kDa protein...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.5.859
更新日期:1999-05-01 00:00:00
abstract::Apoptosis is a major mechanism whereby HIV-1 depletes uninfected CD4+ and CD8+ T cells. We previously showed that resting peripheral blood T cells derived from healthy donors were killed by an apoptotic mechanism after adsorption to gp120-containing, protease-defective HIV-1 (L-2) particles, more effectively than pare...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1453
更新日期:1997-10-01 00:00:00
abstract::An alloreactive CTL clone, 27.B2, produced in the mouse strain combination B6.C-H-2bm1 (bm1) against C57BL/6 (B6) and nominally directed against the Kb molecule has been shown to be peptide dependent. The clone recognizes intact B6, but not intact bm1 or bm3 target cells. However, peptides recognized by 27.B2 CTL can ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.3.321
更新日期:1992-03-01 00:00:00