Abstract:
:Antibody responses to ingested antigens can be inhibited by a mechanism known as oral tolerance which acts to prevent excessive stimulation from luminal contents. Local IgA responses can be induced in this non-responsive environment and during intestinal inflammation, mucosal IgG responses can also be increased. The purpose of this study was to compare a panel of cytokines to factors from macrophage-T cell co-culture supernatants for their ability to enhance isotype and sheep red blood cell (SRBC)-specific plaque-forming cell responses in an in vitro model of oral tolerance. IL-2, IL-4, IL-5, and IL-6, which have been implicated in IgA regulation of lipopolysaccharide-stimulated B cells, were not capable of enhancing responses in tolerized cultures; however, transforming growth factor (TGF)-beta 1 had a dose-dependent ability to enhance responses to the T cell-dependent antigen SRBCs in this system. The enhancement was only seen when antigen was present and was neutralized by specific rabbit antiserum but not normal rabbit IgG. Similar treatment of soluble factors from the macrophage-T cell co-cultures did not inhibit their ability to enhance responses suggesting at least two distinct molecular mechanisms could augment responses in tolerized cultures. This was substantiated further by showing that TGF-beta 1 was not isotype-specific. In contrast, adsorption of the macrophage-T cell co-culture supernatants against monoclonal IgA or IgG removed isotype-specific binding factors which were necessary for the enhancement of IgA and IgG respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Zettel L,Clark DA,Ernst PBdoi
10.1093/intimm/4.9.975subject
Has Abstractpub_date
1992-09-01 00:00:00pages
975-83issue
9eissn
0953-8178issn
1460-2377journal_volume
4pub_type
杂志文章abstract::Lactic acid bacteria (LAB) are abundant in the gastrointestinal tract where they continuously regulate the immune system. NK cells are potently activated by dendritic cells (DCs) matured by inflammatory stimuli, and NK cells are present in the gut epithelium and in mesenteric lymph nodes, but it is not known how NK-DC...
journal_title:International immunology
pub_type: 杂志文章
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abstract::Human intestinal intraepithelial lymphocytes (iIEL) are a unique population of predominantly CD8 alpha beta+, TCR alpha beta+ lymphocytes and, to a lesser extent, TCP gamma delta+ lymphocytes that proliferate poorly to anti-CD3 mitogenic signals but display significant cytolytic activity. Studies in mouse model system...
journal_title:International immunology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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abstract::Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germline band. The same band was also observed in analysis with a 5' DQ probe, a 5'-flanking sequence of DQ52, thus suggesting the use of DQ52. By analyzin...
journal_title:International immunology
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doi:10.1093/intimm/1.6.643
更新日期:1989-01-01 00:00:00
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更新日期:2005-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf079
更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/1.1.99
更新日期:1989-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.3.481
更新日期:1994-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh341
更新日期:2005-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu086
更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/2.10.945
更新日期:1990-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn080
更新日期:2008-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.807
更新日期:1996-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.4.533
更新日期:1996-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.1.39
更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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更新日期:1993-04-01 00:00:00
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更新日期:2001-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/8.9.1421
更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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更新日期:2014-10-01 00:00:00
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更新日期:2002-09-01 00:00:00
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更新日期:2001-06-01 00:00:00
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更新日期:2006-08-01 00:00:00
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更新日期:2003-08-01 00:00:00