Composition of TCR-CD3 complex in human intestinal intraepithelial lymphocytes: lack of Fc epsilon RI gamma chain.


:Human intestinal intraepithelial lymphocytes (iIEL) are a unique population of predominantly CD8 alpha beta+, TCR alpha beta+ lymphocytes and, to a lesser extent, TCP gamma delta+ lymphocytes that proliferate poorly to anti-CD3 mitogenic signals but display significant cytolytic activity. Studies in mouse model systems have shown that the gamma chain of the high-affinity receptor for IgE (Fc epsilon RI gamma) may substitute for the zeta chain in the TCR-CD3 complex of iIEL. This has suggested that the functional properties of these cells may be associated with an altered composition of the TCR-CD3 complex. We therefore analyzed the TCR-CD3 complex of normal human iIEL. One- and two-dimensional non-reducing/reducing SDS-PAGE analysis of CD3 gamma, CD3 delta, CD3 epsilon, zeta and Fc epsilon RI gamma chain immunoprecipitates of cell surface radiolabeled proteins with subunit-specific antibodies revealed a TCR-CD3 complex without associated Fc epsilon RI gamma chains. Thus, normal human iIEL contain a TCR-CD3 complex that consists predominantly of zeta homodimers in association with the alpha beta TCR and CD3 gamma, delta and epsilon, similar to the majority of peripheral lymphocytes. This indicates that the distinct properties of human iIEL are not associated with substitutions of the Fc epsilon RI gamma chain in the TCR-CD3 complex.


Int Immunol


International immunology


Balk SP,Polischuk JE,Probert C,Stevens C,Ebert E,She J,Terhorst C,Blumberg RS




Has Abstract


1995-08-01 00:00:00












  • Rearrangement and expression of kappa light chain genes can occur without mu heavy chain expression during differentiation of pre-B cells.

    abstract::The kinetics of kappa light (kappa L) chain gene rearrangement and expression on mRNA and protein level has been studied with four stromal cell/IL-7 reactive, long-term in vitro proliferating pre-B cell lines and clones, two from fetal liver of normal mice and two from fetal liver of E microH-bcl-2 transgenic (bcl-2-t...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Grawunder U,Haasner D,Melchers F,Rolink A

    更新日期:1993-12-01 00:00:00

  • Nuclear factor of activated T cells contributes to the function of the CD28 response region of the granulocyte macrophage-colony stimulating factor promoter.

    abstract::The granulocyte macrophage colony stimulating factor (GM-CSF) promoter contains a 10 bp element known as CK-1 or CD28RE that specifically responds to the co-stimulatory signal delivered to T cells via the CD28 surface receptor. This element is a variant NFkappaB site that does not function alone but requires an adjace...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Shang C,Attema J,Cakouros D,Cockerill PN,Shannon MF

    更新日期:1999-12-01 00:00:00

  • Modeling the presentation of C3d-coated antigen by B lymphocytes: enhancement by CR1/2-BCR co-ligation is selective for the co-ligating antigen.

    abstract::We have used a set of single-chain variable fragment antibodies (sc) genetically fused with an influenza hemagglutinin-derived peptide as a means to investigate the role of CR1 and CR2 in antigen presentation by B cells. When incubated with the B cell lymphoma 2PK3, peptide-containing sc specific for either CR1 or CR1...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Prechl J,Baiu DC,Horváth A,Erdei A

    更新日期:2002-03-01 00:00:00

  • TLR2-dependent recognition of Streptococcus suis is modulated by the presence of capsular polysaccharide which modifies macrophage responsiveness.

    abstract::Streptococcus suis capsular type 2 is an important swine pathogen and an agent of zoonosis. Although meningitis is the most common form of disease, septicemia and septic shock are also frequently reported. Despite reports that CD14 is involved in the recognition of encapsulated S. suis by host cells, the mechanisms un...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Graveline R,Segura M,Radzioch D,Gottschalk M

    更新日期:2007-04-01 00:00:00

  • Hsp60-mediated T cell stimulation is independent of TLR4 and IL-12.

    abstract::Heat shock protein (Hsp) 60 is thought to function as endogenous danger signal by activating professional antigen-presenting cells (APC) through toll-like receptor (TLR) 4 and CD14, a mechanism that is also used by bacterial LPS. We recently showed that Hsp60 binds LPS and enhances LPS-induced immune stimulation. On t...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Osterloh A,Veit A,Gessner A,Fleischer B,Breloer M

    更新日期:2008-03-01 00:00:00

  • Loss of SDF-1 receptor expression during positive selection in the thymus.

    abstract::SDF-1 is a member of the CXC chemokines. In contrast to other chemokines that are induced by inflammation, SDF-1 is constitutively produced by stromal cells. In order to investigate the physiological roles of SDF-1, we constructed a fusion protein, SDF-1-Cgamma1, composed from murine SDF-1alpha and the constant region...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Suzuki G,Nakata Y,Dan Y,Uzawa A,Nakagawa K,Saito T,Mita K,Shirasawa T

    更新日期:1998-08-01 00:00:00

  • FcγRIIIA-mediated activation of NK cells by IgG heavy chain complexed with MHC class II molecules.

    abstract::Natural killer (NK) cells are a major FcγRIIIA-expressing lymphocyte population that mediate antibody-dependent cellular cytotoxicity. Although NK cells are critical for immunity against viruses and tumors, they are also activated in the joints of patients with rheumatoid arthritis (RA) and may be involved in disease ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Shimizu Y,Kohyama M,Yorifuji H,Jin H,Arase N,Suenaga T,Arase H

    更新日期:2019-04-26 00:00:00

  • LAG-3 is not responsible for selecting T helper cells in CD4-deficient mice.

    abstract::The product of the LAG-3 gene is a cell surface protein with significant homology to CD4. It has been suggested that it can serve as a functional equivalent of CD4 and account for the MHC class II-restricted responses which persist in CD4-deficient mice. To test this hypothesis, we have created CD4/LAG-3 double-defici...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Miyazaki T,Dierich A,Benoist C,Mathis D

    更新日期:1996-05-01 00:00:00

  • Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation.

    abstract::Although there has been extensive analysis on the capacity of MHC-peptide tetramers to bind antigen-specific TCR, there have been comparatively few studies regarding the role of the CD4 and CD8 co-receptors in binding and activation by these multimeric molecules. Here, we start from the observation that different anti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Campanelli R,Palermo B,Garbelli S,Mantovani S,Lucchi P,Necker A,Lantelme E,Giachino C

    更新日期:2002-01-01 00:00:00

  • Differentiation and diversity of subsets in group 1 innate lymphoid cells.

    abstract::NK cells were first identified in 1975 and represent the prototypical group 1 innate lymphoid cell (ILC). More recently, the discovery of new members of the ILC family has highlighted the complexity of this innate lymphoid lineage. Importantly, it has been recognized that different subsets exist within the group 1 ILC...

    journal_title:International immunology

    pub_type: 杂志文章,评审


    authors: Seillet C,Belz GT

    更新日期:2016-01-01 00:00:00

  • The interface between innate and acquired immunity: glycolipid antigen presentation by CD1d-expressing dendritic cells to NKT cells induces the differentiation of antigen-specific cytotoxic T lymphocytes.

    abstract::In vivo administration of NKT cell ligand, alpha-galactosylceramide (alpha-GalCer), caused the activation of NKT cells to induce a strong NK activity and cytokine production by CD1d-restricted mechanisms. Surprisingly, we also found that alpha-GalCer induced the activation of immunoregulatory cells involved in acquire...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nishimura T,Kitamura H,Iwakabe K,Yahata T,Ohta A,Sato M,Takeda K,Okumura K,Van Kaer L,Kawano T,Taniguchi M,Nakui M,Sekimoto M,Koda T

    更新日期:2000-07-01 00:00:00

  • Transmembrane polar residues of TCR beta chain are required for signal transduction.

    abstract::Mutagenic analyses have identified structural motifs important for TCR-mediated signaling in the antigen-binding chains, CD3 and zeta subunits of the TCR complex. In this study, we altered selected residues in the transmembrane and extracellular constant regions of the TCR beta chain and expressed the mutants in a T h...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Fuller-Espie S,Hoffman Towler P,Wiest DL,Tietjen I,Spain LM

    更新日期:1998-07-01 00:00:00

  • O-acetyl GD3 ganglioside in human peripheral blood T lymphocytes.

    abstract::O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Reivinen J,Holthöfer H,Miettinen A

    更新日期:1994-09-01 00:00:00

  • Immunomodulation by hepatitis C virus-derived proteins: targeting human dendritic cells by multiple mechanisms.

    abstract::Hepatitis C virus (HCV) has the ability to persist in the majority of infected people. Strong, multispecific and sustained T-cell response is correlated with viral clearance. The mechanisms of chronicity by HCV are unclear. HCV could restrain the immune system and establish chronic infection by modulating dendritic ce...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Krishnadas DK,Ahn JS,Han J,Kumar R,Agrawal B

    更新日期:2010-06-01 00:00:00

  • Induction of ornithine decarboxylase activity in mouse B lymphocytes.

    abstract::We have examined the role in B cell activation of ornithine decarboxylase (ODC), the labile rate-limiting enzyme in the synthesis of polyamines thought to be required for S phase entry in all cells. When small resting mouse splenic B cells were stimulated with the mitogenic agents phorbol myristate acetate (PMA) plus ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Molitor J,Pace W,Stunz L,Vigna J,Louie S,Ashman R

    更新日期:1994-11-01 00:00:00

  • Allorecognition of human neural stem cells by peripheral blood lymphocytes despite low expression of MHC molecules: role of TGF-beta in modulating proliferation.

    abstract::Neural stem cells (NSCs) transplantation has been proposed as a means of restoring damaged brain tissue, a possibility rendered more likely by reports of low NSCs immunogenicity in various experimental models because of low expression of MHC class I and II as well as co-stimulatory molecules. We investigated the immun...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ubiali F,Nava S,Nessi V,Frigerio S,Parati E,Bernasconi P,Mantegazza R,Baggi F

    更新日期:2007-09-01 00:00:00

  • Cloning and sequence analysis of a novel beta 2-related integrin transcript from T lymphocytes: homology of integrin cysteine-rich repeats to domain III of laminin B chains.

    abstract::The integrins have been grouped into three subfamilies based on the utilization of three distinct beta chain subunits, beta 1, beta 2, and beta 3. The recent discovery of beta 4 and beta 5 subunits, and the sharing of alpha subunits between beta subfamilies reflects a greater structural and functional diversity of the...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yuan QA,Jiang WM,Krissansen GW,Watson JD

    更新日期:1990-01-01 00:00:00

  • Normal D-JH rearranged products of the IgH gene in SCID mouse bone marrow.

    abstract::SCID mice are profoundly immunodeficient, resulting from an inability to carry out the V(D)J recombination reaction during both B cell and T cell development. Recently, however, it was revealed that normal rearrangement frequently did occur in the TCR delta and gamma chain loci in the SCID thymus. To evaluate whether ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Araki R,Itoh M,Hamatani K,Abe M

    更新日期:1996-07-01 00:00:00

  • Signaling events following chemokine receptor ligation in human dendritic cells at different developmental stages.

    abstract::Responsiveness of dendritic cells (DC) to inflammatory CC chemokines is down-regulated during their maturation. We analyzed the mechanism underlying these events. Cell-surface expression of CC chemokine receptor (CCR)-1, -3 and -5 was increased during differentiation of immature DC (iDC) from monocytes. In contrast, t...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Sato K,Kawasaki H,Nagayama H,Enomoto M,Morimoto C,Tadokoro K,Juji T,Takahashi TA

    更新日期:2001-02-01 00:00:00

  • BAFF production by antigen-presenting cells provides T cell co-stimulation.

    abstract::The B cell-activating factor from the tumor necrosis factor family (BAFF) is an important regulator of B cell immunity. Recently, we demonstrated that recombinant BAFF also provides a co-stimulatory signal to T cells. Here, we studied expression of BAFF in peripheral blood leukocytes and correlated this expression wit...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Huard B,Arlettaz L,Ambrose C,Kindler V,Mauri D,Roosnek E,Tschopp J,Schneider P,French LE

    更新日期:2004-03-01 00:00:00

  • Direct evidence for the commitment of hematopoietic stem cells to T, B and myeloid lineages in murine fetal liver.

    abstract::We established an experimental system in vitro to examine the developmental capacity of individual hematopoietic progenitors to generate T, B and myeloid (M) cells. By using this system we analyzed the process of lineage commitment of hematopoietic progenitors in murine fetal liver (FL). It is known that small numbers...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kawamoto H,Ohmura K,Katsura Y

    更新日期:1997-07-01 00:00:00

  • CD154-CD40-independent up-regulation of B7-2 on splenic antigen-presenting cells and efficient T cell priming by staphylococcal enterotoxin A.

    abstract::It has been demonstrated that in vivo T cell priming requires CD154-CD40 interaction, which is suggested to be critical in the induction of co-stimulatory activities on antigen-presenting cells (APC). In the current study, we demonstrate that in vivo administration of a high dose of a superantigen, staphylococcal ente...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Eshima K,Choi Y,Flavell RA

    更新日期:2003-07-01 00:00:00

  • Transendothelial migration of effector T cells across inflamed endothelial barriers does not require heparan sulfate proteoglycans.

    abstract::Leukocyte diapedesis is a chemotactic multistep process that requires optimal chemoattractant presentation by the endothelial barrier. Recent studies have described a critical role for heparan sulfate glycosaminoglycans (HSGAGs) in the presentation and functions of chemokines essential for lymphocyte interactions with...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Stoler-Barak L,Barzilai S,Zauberman A,Alon R

    更新日期:2014-06-01 00:00:00

  • A mouse C kappa-specific T cell clone indicates that DC-SIGN is an efficient target for antibody-mediated delivery of T cell epitopes for MHC class II presentation.

    abstract::In vaccine development, a major objective is to induce strong, specific T cell responses. This might be obtained by targeting antigen to cell surface molecules that efficiently channel the antigen into endocytic compartments for loading of MHC molecules. Antibodies have been used to deliver antigen; however, it is imp...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Schjetne KW,Thompson KM,Aarvak T,Fleckenstein B,Sollid LM,Bogen B

    更新日期:2002-12-01 00:00:00

  • Development of Th1 and not Th2 immune responses in mice lacking IFN-regulatory factor-4.

    abstract::IFN-regulatory factor (IRF)-4 is a member of the IRF family of transcription factors expressed in lymphocytes and macrophages. The previous studies using mice deficient in the IRF-4 gene showed profound defects in function of both B and T cells. To further investigate the role of IRF-4 in CD4(+) T cell function, IRF-4...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Tominaga N,Ohkusu-Tsukada K,Udono H,Abe R,Matsuyama T,Yui K

    更新日期:2003-01-01 00:00:00

  • Evidence for carbohydrate recognition and homotypic and heterotypic binding by the TIM family.

    abstract::The T cell Ig domain and mucin domain (TIM) proteins form a conserved family of transmembrane cell-surface glycoproteins expressed by a variety of tissues. Each TIM protein contains a single V-type Ig domain, a glycosylated mucin-like domain, a transmembrane domain and a cytoplasmic domain. TIM proteins recognize a di...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Wilker PR,Sedy JR,Grigura V,Murphy TL,Murphy KM

    更新日期:2007-06-01 00:00:00

  • The response of human B cells to Mycobacterium leprae. Identification of target antigens following polyclonal activation in vitro.

    abstract::We have investigated the B cell response to Mycobacterium leprae in leprosy patients and healthy controls. A comparison of Western-blotted proteins separated by two-dimensional gel electrophoresis and probed with pooled sera from LL and BT patients revealed distinct antigen recognition patterns for the two classificat...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Mahon AC,Gebre N,Nurlign A

    更新日期:1990-01-01 00:00:00

  • Differential susceptibility of human T(h)1 versus T(h) 2 cells to induction of anergy and apoptosis by ECDI/antigen-coupled antigen-presenting cells.

    abstract::Antigen-coupled antigen-presenting cells (APC) serve as potent tolerogens for inhibiting immune responses in vivo and in vitro, apparently by providing an antigen-specific signal through the TCR in the absence of co-stimulation. Although this approach has been well studied in rodents, little is known about its effects...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Vandenbark AA,Barnes D,Finn T,Bourdette DN,Whitham R,Robey I,Kaleeba J,Bebo BF Jr,Miller SD,Offner H,Chou YK

    更新日期:2000-01-01 00:00:00

  • The extended substrate specificity of the human mast cell chymase reveals a serine protease with well-defined substrate recognition profile.

    abstract::The human chymase (HC) is a major granule constituent of mast cells (MCs) residing in the connective tissue and the sub-mucosa. Although many potential substrates have been described for this important MC enzyme, its full range of in vivo substrates has most likely not yet been identified. A major step toward a better...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Andersson MK,Enoksson M,Gallwitz M,Hellman L

    更新日期:2009-01-01 00:00:00

  • Involvement of 4F2 antigen expressed on the MHC-negative target cells in the recognition of murine CD3+CD4-CD8- alpha beta (V alpha 4/V beta 2) T cells.

    abstract::T cells of an unique phenotype (CD3+CD4-CD8- alpha beta TCR+) develop in vitro from the hematopoietic progenitors, the majority of which carry homologous alpha beta TCR (V alpha 4J alpha 28/V beta 2D beta 1.1J beta 2.6). In the present study, antigen corresponding to the particular alpha beta TCR was investigated, tak...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kubota H,Sato M,Ogawa Y,Iwai K,Hattori M,Yoshida T,Minato N

    更新日期:1994-09-01 00:00:00