Abstract:
:A human neoplastic B cell line SSK41 that expresses IgM on its surface switches spontaneously to IgG-producing cells. The SSK41 line contains a single immunoglobulin heavy-chain locus, the constant region (C) genes of which retain the germline configuration. The IgG-producing SSK41 line was purified by sorting, and shown to have undergone S-S recombination with deletion of the C mu gene. This line produced secretory and membrane-bound forms of gamma-chain mRNA. From cDNA libraries of a mixed population of IgM+/IgG+ SSK41 cells, we have isolated cDNA clones encoding the mature membrane-bound and secretory forms of the mu and gamma 1 heavy chains, all of which share the same variable region sequence. cDNA clones containing the mature gamma 3 chain were identified as well. We also isolated cDNA clones containing C gamma 1 and C gamma 3 sterile transcripts from the SSK41 line. These sterile transcripts contained additional exon sequences designated 'I' which were localized upstream of the C gamma 1 and C gamma 3 switch regions and homologous to murine counterparts. The I sequences were precisely spliced to the 5' ends of the corresponding C gamma exon sequences. These features of germline CH transcripts, i.e. the isotype specificity to class switching, location of exons, and sequences per se, are highly conserved between man and mouse.
journal_name
Int Immunoljournal_title
International immunologyauthors
Sideras P,Mizuta TR,Kanamori H,Suzuki N,Okamoto M,Kuze K,Ohno H,Doi S,Fukuhara S,Hassan MSdoi
10.1093/intimm/1.6.631subject
Has Abstract,Author List Incompletepub_date
1989-01-01 00:00:00pages
631-42issue
6eissn
0953-8178issn
1460-2377journal_volume
1pub_type
杂志文章abstract::The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle control or cellular response to...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf060
更新日期:2002-08-01 00:00:00
abstract::TCR engagement in the thymus results in both survival and elimination signals for developing thymocytes. To examine whether both signals can be provided by the same cell type, we investigated the ability of a thymic epithelial cell (TEC) line 427.1, previously shown to allow positive selection in the thymus, to induce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.2.239
更新日期:1994-02-01 00:00:00
abstract::T cells of an unique phenotype (CD3+CD4-CD8- alpha beta TCR+) develop in vitro from the hematopoietic progenitors, the majority of which carry homologous alpha beta TCR (V alpha 4J alpha 28/V beta 2D beta 1.1J beta 2.6). In the present study, antigen corresponding to the particular alpha beta TCR was investigated, tak...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1323
更新日期:1994-09-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) signaling pathway is strongly activated in response to TCR stimulation in normal T cells. However, the extent to which activation of the ERK pathway is necessary for TCR-stimulated cytokine production is not clear. We have addressed this question by use of two separate m...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.2.223
更新日期:1998-02-01 00:00:00
abstract::We examined effects of c-fos overexpression on the development and property of peritoneal B-1 cells using transgenic (H2-c-fos) mice carrying the c-fos gene under the control of the constitutive H-2Kb promoter. The number of B-1b cells in the peritoneal cavity of H2-c-fos mice was 4-fold larger than that in control li...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh149
更新日期:2004-10-01 00:00:00
abstract::In this study, we analyzed peripheral blood CD4+CD8+ double-positive (DP) lymphocytes in adult cynomolgus monkeys (Macaca fascicularis). Forty of 55 monkeys had > 5% of the peripheral blood DP subpopulation (9.3 +/- 5.9%; mean +/- SD) in peripheral blood lymphocytes (PBL) in contrast to a low percentage of peripheral ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.4.591
更新日期:1997-04-01 00:00:00
abstract::Susceptibility and resistance to type 1 diabetes are associated with MHC class II alleles that carry non-Asp and Asp at residue 57 of their beta chain respectively. The effect of Asp or non-Aspbeta57 may relate to a differential ability of distinct class II molecules to bind specific immuno-pathogenic peptides. Recent...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.8.1229
更新日期:1998-08-01 00:00:00
abstract::NOD mice spontaneously develop type I diabetes resulting from autoimmune destruction of their insulin-producing beta cells. Among the self-antigens targeted by NOD autoimmune T cells is a peptide, p277, from the sequence of the 60 kDa heat shock protein (hsp60). Common to the anti-p277 T cell populations of NOD mice i...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.6.957
更新日期:1999-06-01 00:00:00
abstract::The profile of cytokines produced by CD4 T cells is profoundly influenced by the presence of IL-12. Here we demonstrate that during re-stimulation of antigen-specific immune responses in vitro, antigen-primed lymph node cells from DBA/2 mice produced 3- to 30-fold more IL-12 than did cells from BALB/c mice, which are ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.10.1511
更新日期:1996-10-01 00:00:00
abstract::HIV infected T and monocytic cell lines could activate and fix C3 fragments when incubated in human serum under conditions allowing for activation of the alternative complement pathway. Normal T lymphocytes incubated with HIV could also activate and fix C3. This activity was, at least in part, the property of the viru...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.4.395
更新日期:1991-04-01 00:00:00
abstract::Leptin, one of the typical adipokines, is reported to promote Th17 cell responses and to enhance production of proinflammatory cytokines. To clarify the role of leptin in the regulation of the IL-23/IL-17 axis and the development of kidney disease, we used a murine model of nephrotoxic serum (NTS) nephritis (NTN). She...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxv067
更新日期:2016-04-01 00:00:00
abstract::Two major B cell subpopulations were identified in the IgD- compartment of tonsils and subsequently isolated. They displayed the following phenotypes: CD10+CD38+CD44- (CD38+ B cells) and CD10-CD38-CD44+ (CD38- B cells). Of the CD38- B cells, 70% also expressed CD24 and CD39, whereas CD77 was specifically distributed o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1259
更新日期:1993-10-01 00:00:00
abstract::It has proven difficult to evaluate the functional potential of germinal center (GC) B cells, including those from Peyer's patches (PP), by either in vivo or in vitro methods. Thus, rather than assess secreted Ig product as an indicator of functional potential we have instead sought to detect mRNAs related to the vari...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.12.1253
更新日期:1991-12-01 00:00:00
abstract::Amongst CD4-CD8- (double negative) thymocytes there is a sizeable population (variable from strain to strain) of cells expressing surface T cell receptor (TCR). These TCR+ double negatives are predominantly non-cycling, have very little precursor activity, and, unlike the TCR-CD4-CD8- thymocytes, appear not to be part...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.1.51
更新日期:1990-01-01 00:00:00
abstract::Streptococcus suis serotype 2 is an important porcine bacterial pathogen and a zoonotic agent responsible for sudden death, septic shock and meningitis, with exacerbated inflammation being a hallmark of the systemic and central nervous system (CNS) infections. However, S. suis serotype 2 strains are genetically and ph...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz033
更新日期:2019-10-16 00:00:00
abstract::We demonstrate here that human necrosis factor-alpha, a potent neutrophil activator, induces rapid (within 3 h) apoptosis of these cells, i.e. neutrophils treated with this cytokine exhibit (i) light and electron microscopic changes characteristic to apoptotic cells, (ii) reduced propidium iodide binding to DNA, and (...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.691
更新日期:1993-06-01 00:00:00
abstract::Chronic viral infections are often characterized by CD8 T-cell responses with poor cytokine secretion potential and limited expansion of the CD8 T-cell pool, collectively referred to as CD8 T-cell exhaustion. Exhaustion of lymphocytic choriomeningitis virus (LCMV)-specific CD8 T cells was shown to be partially regulat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp107
更新日期:2010-01-01 00:00:00
abstract::Recent studies have shown that tumor cells acquire escape mechanisms to evade host immunity in the tumor microenvironment. Two key immune checkpoint pathways mediated by immunosuppressive co-signaling, the first via programmed cell death 1 (PD-1) and PD-1 ligand 1 (PD-1/PD-L1) and the second via CTLA-4 and B7 (CTLA-4/...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxw020
更新日期:2016-07-01 00:00:00
abstract::The alternative complement pathway (ACP) in vertebrates is known to be important in inflammatory reactions, and to be activated by foreign substances such as bacterial lipopolysaccharide (LPS) and zymosan, although to date no intrinsic activators have been identified except complement receptor type 2. From the point o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1349
更新日期:1993-10-01 00:00:00
abstract::The previously defined binding motif of MHC class I H-2Kd-restricted antigenic peptides consists of a Y residue in position P2 and a hydrophobic residue with a large aliphatic side chain (L, I, or V) in position P9/P10 of optimal 9- or 10-mer peptides. We show now that the presence of a charged or a F residue in posit...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.11.1489
更新日期:1993-11-01 00:00:00
abstract::The mechanism underlying selective IgG subclass deficiency is largely unknown in humans. We have previously reported the acquisition of a complete IgG2 deficiency in a leukemia patient after bone marrow transplantation. Southern blot analysis showed a deletion including the Cgamma2 and Cgamma4 genes on one chromosome ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh192
更新日期:2005-01-01 00:00:00
abstract::Antigen engagement of the TCR may lead to activation of mature T cells while inducing deletion or positive selection of immature thymocytes. Using thymocytes from TCR transgenic mice recognizing the allo-antigen H-2Kb we investigated whether double-positive CD4+CD8+ (DP) thymocytes constitute a particular developmenta...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.9.1421
更新日期:1996-09-01 00:00:00
abstract::Converging data suggest an important role for IL-7 in T lymphocyte maturation as illustrated by the severe T lymphopenia observed in IL-7-deficient mice. We recently reported that IL-7 preferentially promotes the in vitro expansion of a discrete MHC class I-dependent lymphocyte subset comprising both CD4+ and CD4-CD8-...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.11.1751
更新日期:1996-11-01 00:00:00
abstract::A small proportion of the sIg+ B lymphocytes in peripheral lymphoid organs [22% in spleen and 6% in lymph node (LN)] in rat carries the Thy-1 antigen. These Thy-1+ B cells represent newly formed bone marrow (BM) derived (or immature) B cells. In this study we investigated the kinetic behavior of Thy-1+ and Thy-1- B ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.7.735
更新日期:1993-07-01 00:00:00
abstract::Mice lacking the IL-2 receptor beta chain (IL-2R beta) exhibit an autoimmune reaction characterized by generalized T cell activation, production of autoantibodies, myeloproliferation and severe anemia. T cells of IL-2R beta-/- mice were examined to elucidate the mechanism responsible for their abnormal activation and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.9.1367
更新日期:1997-09-01 00:00:00
abstract::Animal models of autoimmune diseases have been instrumental in advancing our understanding of autoimmunity in humans. Collagen-induced arthritis in mice is an autoimmune disease model of rheumatoid arthritis, which is MHC class II restricted and CD4 T cell dependent. To better understand the fundamental role of T cell...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.11.1613
更新日期:1998-11-01 00:00:00
abstract::The role of B7 co-stimulatory signaling in in vivo tumor rejection remains incompletely characterized. In particular, the relative competence of B7-1 (CD80) and B7-2 (CD86) to provide effective co-stimulus is not well defined, and the identification of the T cell co-stimulatory receptor that mediates B7 co-stimulation...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.6.791
更新日期:1998-06-01 00:00:00
abstract::Constitutive expression of p35 and p40 IL-12 mRNA was detected in splenic macrophages isolated from aged mice. Macrophages were also implicated as the cell type responsible for the dysregulated IL-6 and tumor necrosis factor (TNF)-alpha commonly observed to be constitutively produced by lymphoid cells from aged donors...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.5.745
更新日期:1997-05-01 00:00:00
abstract::We have previously cloned the murine homolog of cDNA for the human myelomonocytic differentiation antigen, CD14. We synthesized three hydrophilic peptides derived from the predicted amino acid sequence of murine CD14 (mCD14), designated MS7.1, MS7.2, and MS7.3, respectively, and raised antisera against them. Each anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.2.205
更新日期:1991-02-01 00:00:00
abstract::Immune tolerance in the lung is important for preventing hypersensitivity, such as allergic asthma. Maintenance of tolerance in the lung is established by coordinated activities of poorly understood cellular and molecular mechanisms, including participation of dendritic cells (DCs). We have previously identified DC ex...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxx011
更新日期:2017-02-01 00:00:00