Abstract:
:Animal models of autoimmune diseases have been instrumental in advancing our understanding of autoimmunity in humans. Collagen-induced arthritis in mice is an autoimmune disease model of rheumatoid arthritis, which is MHC class II restricted and CD4 T cell dependent. To better understand the fundamental role of T cells in arthritis, we have generated a transgenic mouse carrying the rearranged Valpha11.1 and Vbeta8.2 TCR chain genes isolated from a type II collagen (CII)-specific T cell hybridoma. Cell surface analysis indicated that Vbeta8.2 chain was expressed on the surface of nearly all peripheral T cells. Analysis of T cell subsets in transgenic mice revealed a profound skewing in peripheral T cells towards the CD4 population. Although peripheral T cells were not tolerant to CII and responded to CII stimulation in vitro, transgenic mice did not develop spontaneous arthritis. However, a rapid onset of arthritis with severe clinical signs was detected in transgenic mice after immunization with CII in complete Freund's adjuvant. Histological analysis of inflamed joints showed a great resemblance to arthritic joints in man. This unique transgenic mouse model provides valuable insights into the mechanism of arthritis and into potential specific immune interventions.
journal_name
Int Immunoljournal_title
International immunologyauthors
Osman GE,Cheunsuk S,Allen SE,Chi E,Liggitt HD,Hood LE,Ladiges WCdoi
10.1093/intimm/10.11.1613subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1613-22issue
11eissn
0953-8178issn
1460-2377journal_volume
10pub_type
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更新日期:1991-04-01 00:00:00
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更新日期:1995-08-01 00:00:00
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更新日期:1991-02-01 00:00:00
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