Abstract:
:Recombination activating genes Rag-1 and Rag-2 were isolated on the basis of their ability to confer V(D)J recombination activity when co-expressed in fibroblasts. The mode of action of the confer V(D)J recombination activity when co-expressed in fibroblasts. The mode of action of the products of these genes is not known. Based on sequence comparison data, it was suggested that Rag-1 protein could act like a topoisomerase and that tyrosine in position 998 could be the active site tyrosine. We tested this hypothesis by introducing a point mutation on the Rag-1 cDNA, transforming the tyrosine codon into a phenylalanine codon. We show that the mutation has no effect on site specific recombination implying that Tyr-998 is not essential for the recombination reaction.
journal_name
Int Immunoljournal_title
International immunologyauthors
Kallenbach S,Brinkmann T,Rougeon Fdoi
10.1093/intimm/5.2.231subject
Has Abstractpub_date
1993-02-01 00:00:00pages
231-2issue
2eissn
0953-8178issn
1460-2377journal_volume
5pub_type
杂志文章abstract::Cross-linking of the IgM antigen receptor on an immature B cell lymphoma (BKS-2) induces growth arrest and apoptosis. This is accompanied by down-regulation of the immediate early genes, egr-1 and c-myc, and a reduction in NF-kappaB activity. Anti-IgM-induced growth arrest and apoptosis of this murine B cell lymphoma ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.6.871
更新日期:1999-06-01 00:00:00
abstract::Novel biomarkers of rheumatoid arthritis (RA), in addition to antibodies against cyclic citrullinated peptides, are required. Metabolome analysis is a promising approach to identify metabolite biomarkers for clinical diagnosis. We adopted a comprehensive nontargeted metabolomics approach combining capillary electropho...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxaa059
更新日期:2020-08-31 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh029
更新日期:2004-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.10.1625
更新日期:1999-10-01 00:00:00
abstract::Liver fibrosis is commonly observed in chronic liver disease. However, the immunological mechanisms underlying hepatic fibrosis due to chronic inflammation are not well defined, mainly because suitable experimental models have not been established. We have found that weekly i.v. administration of concanavalin A (Con A...
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pub_type: 杂志文章
doi:10.1093/intimm/11.9.1491
更新日期:1999-09-01 00:00:00
abstract::A central tenet of vaccine development is to identify immune correlates of protection. Both plasmid-encoded gD as well as recombinant protein gD can protect mice from lethal herpes simplex virus (HSV) challenge. It is known that different vaccine modalities should induce different immune phenotypes. Yet, paradoxically...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.11.1763
更新日期:1999-11-01 00:00:00
abstract::Exosomes are small vesicles (60-100 nm) secreted by various cell types upon the fusion of endosomal compartments with the plasma membrane. Exosomes from antigen-presenting cells (APC), such as B lymphocytes and dendritic cells (DC), bear MHC class II molecules. In addition, the injection of DC-derived exosomes was rep...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf048
更新日期:2002-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.4.339
更新日期:2002-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1545
更新日期:1997-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf047
更新日期:2002-07-01 00:00:00
abstract::Recent studies point to glycolipid-enriched membrane (GEM) microdomains as the critical sites for TCR-mediated signal transduction. However, whether the TCR complex is localized in the GEM domain is not well-defined. In the present study, we analyzed localization of the TCR-CD3 complex in the GEM domain by isolating t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1395
更新日期:1999-09-01 00:00:00
abstract::Immunoglobulin (IG:) genes are hypermutated in mature B cells after interaction with antigen and T cells in a germinal center reaction. We and others have recently shown that the human BCL6 gene is also hypermutated in human peripheral blood memory B cells and tonsils. A preliminary analysis of other non-Ig genes (c-M...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.7.1085
更新日期:2000-07-01 00:00:00
abstract::The endocytic and phagocytic activities of a population of MHC IIhi CD11c+ dendritic cell (DC)-like cells in synovium-rich tissues (SRTs) of normal rat paws were compared with CD163+ cells (putative macrophages) from the same tissues and pseudo-afferent lymph DCs, peritoneal macrophages and blood monocytes. Fifty perc...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh291
更新日期:2005-08-01 00:00:00
abstract::Animal models of autoimmune diseases have been instrumental in advancing our understanding of autoimmunity in humans. Collagen-induced arthritis in mice is an autoimmune disease model of rheumatoid arthritis, which is MHC class II restricted and CD4 T cell dependent. To better understand the fundamental role of T cell...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.11.1613
更新日期:1998-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.691
更新日期:1993-06-01 00:00:00
abstract::At birth, virtually all peripheral CD8(+) T cells express the CD28 co-stimulatory molecule, but healthy human adults accumulate CD28(-)CD8(+) T cells that often express the CD57 marker. While these CD28(-) subpopulations are known to exert effector-type functions, the generation, maintenance and regulation of CD28(-) ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.8.1327
更新日期:1999-08-01 00:00:00
abstract::We examined effects of c-fos overexpression on the development and property of peritoneal B-1 cells using transgenic (H2-c-fos) mice carrying the c-fos gene under the control of the constitutive H-2Kb promoter. The number of B-1b cells in the peritoneal cavity of H2-c-fos mice was 4-fold larger than that in control li...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh149
更新日期:2004-10-01 00:00:00
abstract::CT1 is a carbohydrate moiety of CD45 that is expressed on fetal thymocytes in vivo. Examination of CT1 expression on thymocyte subsets revealed that primarily pro-T cells (CD44+ CD25+) and pre-T cells (CD44- CD25+) expressed CT1. Interestingly, non-T-lineage committed lymphoid progenitors (CD44+ CD25-) lacked CT1 indi...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.4.445
更新日期:1998-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn009
更新日期:2008-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl065
更新日期:2006-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.3.415
更新日期:1997-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.5.725
更新日期:1996-05-01 00:00:00
abstract::Human dendritic cells (DC) generated from CD34+ hematopoietic progenitors cultured in the presence of granulocyte macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha are related to Langerhans cells (DLC) and have been shown to induce a strong proliferation of allogeneic CD4+ T cells. Th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1177
更新日期:1994-08-01 00:00:00
abstract::Specific antibodies increase antigen uptake and presentation by antigen-presenting cells via the B cell receptor in B cells or FcgammaR in dendritic cells. To determine whether the interaction between antibody and antigen could influence the set of peptides presented by MHC II molecules, we analyzed the presentation o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.11.1863
更新日期:1999-11-01 00:00:00
abstract::The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle control or cellular response to...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf060
更新日期:2002-08-01 00:00:00
abstract::MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.5.625
更新日期:1996-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxy067
更新日期:2019-02-15 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu054
更新日期:2014-10-01 00:00:00
abstract::The specificity of self MHC-restricted T cells responding to peptide fragments of processed antigen has been well characterized. However, the means by which alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We now provide evidence that the recognition of class I human leukocyte antigen (HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.1.103
更新日期:1993-01-01 00:00:00
abstract::We have previously shown that the occurrence of CD45RA+ adult mouse thymocytes is strain-dependent, e.g. constituting approximately 0.6% in C57BL/Icrf and approximately 2.5% in BALB/c (Huby, R. and Goff, L., 1992. Eur. J. Immunol. 22:1659). Here we show that irrespective of strain, the thymus contains approximately 0....
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.11.1303
更新日期:1992-11-01 00:00:00