Abstract:
:The interleukin 2 receptor (IL-2R) is composed of at least two polypeptides--a 55 kd protein (the L chain, p55, or alpha chain) and a 75 kd protein (the H chain, p75 or beta chain). The high-affinity binding of IL-2 results in the formation of a ternary complex consisting of IL-2 and the L and H chains. We found that the rate of high-affinity complex formation at 0 degrees C was about one-third of that at 37 degrees C. The reduction of high-affinity complex formation rate at lower temperatures correlates with the reduction of lateral diffusion within the membrane at lower temperatures. An anti-H chain antibody (2RB) inhibited the formation of the high-affinity complex at 0 degrees C but not at 37 degrees C. We studied the kinetics of the high-affinity complex formation at 37 degrees C after the preincubation of ATL-2 cells with the 2RB antibody and IL-2 at 0 degrees C. We found that the amount of IL-2.L complex formed initially at 0 degrees C was almost equivalent to the amount of high-affinity complex formed by subsequent incubation at 37 degrees C. These results suggest that the IL-2.L complex might be converted to the high-affinity IL-2R complex. Similar experiments using YTC3 cells, which express a smaller number of L chains, showed slower rates of high-affinity complex formation, in agreement with the affinity conversion/stepwise binding model.
journal_name
Int Immunoljournal_title
International immunologyauthors
Saito Y,Ogura T,Kamio M,Sabe H,Uchiyama T,Honjo Tdoi
10.1093/intimm/2.12.1167subject
Has Abstractpub_date
1990-01-01 00:00:00pages
1167-77issue
12eissn
0953-8178issn
1460-2377journal_volume
2pub_type
杂志文章abstract::Taking advantage of the recently demonstrated presence of N-aminopeptidases and the serine protease dipeptidyl aminopeptidase IV (DPP IV) at the surface of human myeloblastic HL-60 cells, the regulation of these protease activities in HL-60 cell differentiation has been assessed using combined spectrophotometric and f...
journal_title:International immunology
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pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.1.21
更新日期:1999-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh255
更新日期:2005-06-01 00:00:00
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更新日期:2001-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh381
更新日期:2006-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/10.3.325
更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.12.1253
更新日期:1991-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.5.619
更新日期:1998-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxr123
更新日期:2012-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:International immunology
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更新日期:1999-03-01 00:00:00
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更新日期:2004-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxw020
更新日期:2016-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs109
更新日期:2013-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.591
更新日期:1993-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.5.509
更新日期:1989-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh134
更新日期:2004-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh139
更新日期:2004-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.3.387
更新日期:1994-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs069
更新日期:2012-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.12.1945
更新日期:1999-12-01 00:00:00