Differential effect of insulin and elevated glucose level on adenosine transport in rat B lymphocytes.


:Impaired lymphocyte function is a common feature of human diabetes. Nucleoside transport across the plasma membrane is an essential step during lymphocyte growth and activation. In our study, we evaluated the impact of diabetic conditions on nucleoside transport system in B lymphocytes. Examination of the nucleoside transporters expression level in B lymphocytes isolated from diabetic rats revealed significant changes in their mRNA levels. Experiments performed on B cells cultured in medium containing defined concentrations of glucose and insulin showed that the rENT1 mRNA level was sensitive to extracellular glucose concentration and was not affected by insulin. Increase of glucose concentration from 5 to 20 mM caused a decrease of rENT1 mRNA by 80% and was associated with decreased adenosine uptake by the B cells. The effect of glucose was blocked by PD98059, a MAPK kinase inhibitor. The mRNA levels of rENT2 and rCNT2 were highly dependent on insulin but not on glucose concentration. Exposure of lymphocytes to 10 nM insulin resulted in a 2-fold increase in rENT2 mRNA and a 50% decrease in the rCNT2 mRNA level. Alterations in mRNA levels of rENT2 and rCNT2 were associated with changes in adenosine transport. Insulin-induced changes in expression level of rENT2 were blocked by wortmannin, an inhibitor of phosphatidylinositide 3-kinase, whereas the effect of insulin on rCNT2 was inhibited by PD98059 and to a lesser extend by wortmannin. In summary, impaired nucleoside transport in diabetic B lymphocytes results from alterations in the expression of nucleoside transporters, which are independently and differentially regulated by glucose and insulin.


Int Immunol


International immunology


Sakowicz M,Szutowicz A,Pawelczyk T




Has Abstract


2005-02-01 00:00:00














  • On the mechanism of human T cell suppression.

    abstract::Previous evidence from several laboratories suggests that CD8+ T suppressor cells may be important regulatory elements governing specific unresponsiveness of lepromatous lepromatous leprosy patients to M.leprae. To analyse the mechanism of suppression, CD8+ Ts clones were established from lesions and peripheral blood ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Salgame P,Modlin R,Bloom BR

    更新日期:1989-01-01 00:00:00

  • IL-1α released from oral epithelial cells upon candidalysin exposure initiates an early innate epithelial response.

    abstract::Candida albicans is a commensal fungus that predominantly resides on mucosal surfaces and can cause lethal systemic infection when the host defense is compromised. Candidalysin is a cytolytic peptide toxin produced by C. albicans hyphae that is essential for mucosal tissue damage and is believed to contribute to the e...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hanaoka M,Domae E

    更新日期:2020-10-10 00:00:00

  • V beta 6-bearing T cells are involved in resistance to Trypanosoma cruzi infection in XID mice.

    abstract::BALB.xid mice, carrying an X-linked mutation leading to the absence of CD5+ B cells, are highly resistant to Trypanosoma cruzi infection. These mice clear blood parasites in the acute phase of infection and do not develop the inflammatory infiltration characteristically observed in the chronic phase of susceptible str...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cordeiro da Silva A,Lima EC,Vicentelli MH,Minoprio P

    更新日期:1996-08-01 00:00:00

  • Developmental control of antigen-induced thymic transcription factors.

    abstract::Antigen engagement of the TCR may lead to activation of mature T cells while inducing deletion or positive selection of immature thymocytes. Using thymocytes from TCR transgenic mice recognizing the allo-antigen H-2Kb we investigated whether double-positive CD4+CD8+ (DP) thymocytes constitute a particular developmenta...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Simon AK,Auphan N,Schmitt-Verhulst AM

    更新日期:1996-09-01 00:00:00

  • Distinct indirect pathways govern human NK-cell activation by TLR-7 and TLR-8 agonists.

    abstract::NK cells limit the emergence of cancers and viral infections by surveillance of 'missing-self' and 'induced-self' ligands, and by direct recognition of pathogen-associated molecules. We examined individual roles for Toll-like receptors (TLRs)-7 and -8 in human NK-cell activation using synthetic, small molecule agonist...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Gorski KS,Waller EL,Bjornton-Severson J,Hanten JA,Riter CL,Kieper WC,Gorden KB,Miller JS,Vasilakos JP,Tomai MA,Alkan SS

    更新日期:2006-07-01 00:00:00

  • Expression of CD45 isoforms by fresh and activated human gamma delta T lymphocytes and natural killer cells.

    abstract::Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, a...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Braakman E,Sturm E,Vijverberg K,van Krimpen BA,Gratama JW,Bolhuis RL

    更新日期:1991-07-01 00:00:00

  • Prostaglandin E2 is a major soluble factor produced by stromal cells for preventing inflammatory cytokine production from dendritic cells.

    abstract::Dendritic cells (DCs) are specialized antigen-presenting cells that play pivotal roles in initiating immune responses. However, DC maturation is usually strongly restricted by the stromal microenvironment, especially in non-lymphoid tissues, such as skin and mucosa. Although suppression of DC maturation by stromal cel...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Shiraishi H,Yoshida H,Saeki K,Miura Y,Watanabe S,Ishizaki T,Hashimoto M,Takaesu G,Kobayashi T,Yoshimura A

    更新日期:2008-09-01 00:00:00

  • Gp49B is a pathogenic marker for auto-antibody-producing plasma cells in lupus-prone BXSB/Yaa mice.

    abstract::AbstractImmune homeostasis is critically regulated by the balance between activating and inhibitory receptors expressed on various immune cells such as T and B lymphocytes, and myeloid cells. The inhibitory receptors play a fundamental role in the immune checkpoint pathway, thus maintaining peripheral tolerance. We re...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Wong YL,Su MT,Sugahara-Tobinai A,Itoi S,Kezuka D,Endo S,Inui M,Takai T

    更新日期:2019-05-21 00:00:00

  • Truncation of the mu heavy chain alters BCR signalling and allows recruitment of CD5+ B cells.

    abstract::Ig are multifunctional molecules with distinct properties assigned to individual domains. To assess the importance of IgM domain assembly in B cell development we generated two transgenic mouse lines with truncated muH chains by homologous integration of the neomycin resistance gene (neo(r)) into exons C(mu)1 and C(mu...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Zou X,Ayling C,Xian J,Piper TA,Barker PJ,Brüggemann M

    更新日期:2001-12-01 00:00:00

  • S1P lyase in thymic perivascular spaces promotes egress of mature thymocytes via up-regulation of S1P receptor 1.

    abstract::Sphingosine 1-phosphate (S1P) and S1P receptor 1 (S1P1) play an important role in the egress of mature CD4 or CD8 single-positive (SP) thymocytes from the thymus. Fingolimod hydrochloride (FTY720), an S1P1 functional antagonist, induced significant accumulation of CD62L(high)CD69(low) mature SP thymocytes in the thymi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Maeda Y,Yagi H,Takemoto K,Utsumi H,Fukunari A,Sugahara K,Masuko T,Chiba K

    更新日期:2014-05-01 00:00:00

  • TRAF1 regulates Th2 differentiation, allergic inflammation and nuclear localization of the Th2 transcription factor, NIP45.

    abstract::We have previously reported that tumor necrosis factor receptor-associated factor 1 (TRAF1), an intracellular protein, which binds to a range of molecules, including tumor necrosis factor (TNF) receptor family members, regulates TNF-induced NF-kappaB and AP-1 signaling as well as TCR-triggered proliferative responses ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Bryce PJ,Oyoshi MK,Kawamoto S,Oettgen HC,Tsitsikov EN

    更新日期:2006-01-01 00:00:00

  • Dendritic cell expression of the signaling molecule TRAF6 is required for immune tolerance in the lung.

    abstract::Immune tolerance in the lung is important for preventing hypersensitivity, such as allergic asthma. Maintenance of tolerance in the lung is established by coordinated activities of poorly understood cellular and molecular mechanisms, including participation of dendritic cells (DCs). We have previously identified DC ex...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Han D,Walsh MC,Kim KS,Hong SW,Lee J,Yi J,Rivas G,Choi Y,Surh CD

    更新日期:2017-02-01 00:00:00

  • Over-expressed IgG2 antibodies against O-acetylated sialoglycoconjugates incapable of proper effector functioning in childhood acute lymphoblastic leukemia.

    abstract::Earlier studies have demonstrated an over-expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on lymphoblasts, concomitant with high titers of anti-9-OAcSGs in childhood acute lymphoblastic leukemia (ALL). The present study was aimed to evaluate whether this high induction of anti-9-OAcSGs at disease presenta...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Bandyopadhyay S,Bhattacharyya A,Mallick A,Sen AK,Tripathi G,Das T,Sa G,Bhattacharya DK,Mandal C

    更新日期:2005-02-01 00:00:00

  • Involvement of 4F2 antigen expressed on the MHC-negative target cells in the recognition of murine CD3+CD4-CD8- alpha beta (V alpha 4/V beta 2) T cells.

    abstract::T cells of an unique phenotype (CD3+CD4-CD8- alpha beta TCR+) develop in vitro from the hematopoietic progenitors, the majority of which carry homologous alpha beta TCR (V alpha 4J alpha 28/V beta 2D beta 1.1J beta 2.6). In the present study, antigen corresponding to the particular alpha beta TCR was investigated, tak...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kubota H,Sato M,Ogawa Y,Iwai K,Hattori M,Yoshida T,Minato N

    更新日期:1994-09-01 00:00:00

  • The P9 pocket of HLA-DQ2 (non-Aspbeta57) has no particular preference for negatively charged anchor residues found in other type 1 diabetes-predisposing non-Aspbeta57 MHC class II molecules.

    abstract::Susceptibility and resistance to type 1 diabetes are associated with MHC class II alleles that carry non-Asp and Asp at residue 57 of their beta chain respectively. The effect of Asp or non-Aspbeta57 may relate to a differential ability of distinct class II molecules to bind specific immuno-pathogenic peptides. Recent...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Quarsten H,Paulsen G,Johansen BH,Thorpe CJ,Holm A,Buus S,Sollid LM

    更新日期:1998-08-01 00:00:00

  • Leptin deficiency down-regulates IL-23 production in glomerular podocytes resulting in an attenuated immune response in nephrotoxic serum nephritis.

    abstract::Leptin, one of the typical adipokines, is reported to promote Th17 cell responses and to enhance production of proinflammatory cytokines. To clarify the role of leptin in the regulation of the IL-23/IL-17 axis and the development of kidney disease, we used a murine model of nephrotoxic serum (NTS) nephritis (NTN). She...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Goto K,Kaneko Y,Sato Y,Otsuka T,Yamamoto S,Goto S,Yamamoto K,Yamamoto T,Kawachi H,Madaio MP,Narita I

    更新日期:2016-04-01 00:00:00

  • Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells.

    abstract::The TCR complex transduces the antigen recognition signal through common activation motifs present in both CD3 gamma delta epsilon chains and zeta dimers within the complex. We have investigated functional roles of the cytoplasmic domain in zeta and CD3 gamma delta epsilon for T cell activation in early and late respo...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Aoe T,Goto S,Ohno H,Saito T

    更新日期:1994-11-01 00:00:00

  • Elevated expression levels of an Ig transgene in mice links the IgH 3' enhancer to the regulation of IgH expression.

    abstract::To delineate the role of the IgH 3' enhancer in the regulation of Ig heavy (IgH) chain gene expression, mice harbouring rearranged IgH transgenes, with (PSVmu3) and without (PSVmu1) this element, were produced. RNA and protein analysis from the different transgenic lines revealed a 5- to 7-fold increase in the express...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Arulampalam V,Furebring C,Samuelsson A,Lendahl U,Borrebaeck C,Lundkvist I,Pettersson S

    更新日期:1996-07-01 00:00:00

  • Immune regulatory effects of central nervous system antigens in culture.

    abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lindsey JW,Jin R

    更新日期:2000-11-01 00:00:00

  • Redistribution of selectin counter-ligands induced by cytokines.

    abstract::Soluble recombinant (r) P-selectin and rP-selectin immobilized on plastic surfaces were tested for their capacity to activate neutrophils to produce superoxide anion. Soluble rP-selectin was incapable of activating leukocytes, whereas immobilized rP-selectin was able to induce leukocyte activation. When neutrophils we...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nagata K,Tsuji T,Matsushima K,Hanai N,Irimura T

    更新日期:2000-04-01 00:00:00

  • Alternative complement pathway activation by HIV infected cells: C3 fixation does not lead to complement lysis but enhances NK sensitivity.

    abstract::HIV infected T and monocytic cell lines could activate and fix C3 fragments when incubated in human serum under conditions allowing for activation of the alternative complement pathway. Normal T lymphocytes incubated with HIV could also activate and fix C3. This activity was, at least in part, the property of the viru...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yefenof E,Asjö B,Klein E

    更新日期:1991-04-01 00:00:00

  • The involvement of the proto-oncogene p120 c-Cbl and ZAP-70 in CD2-mediated T cell activation.

    abstract::The CD2 co-receptor expressed on the surface of T lymphocytes is able to stimulate T cell activation, proliferation and cytokine production in the absence of direct engagement of the antigen-specific TCR. Engagement of human CD2 by mitogenic pairs of anti-CD2 mAb induces tyrosine phosphorylation of a number of intrace...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lin H,Martelli MP,Bierer BE

    更新日期:2001-01-01 00:00:00

  • Requirement of high-affinity IL-2-IL-2R interaction for T cell anergy induction.

    abstract::Incomplete T cell antigen receptor-mediated signaling induces an unresponsive state known as anergy. Previously, we had shown that anergy can be induced in antigen-primed but not naive T cells. In this report, we found that in vitro primed T cells from IL-2R alpha-deficient mice were resistant to anergy induction in c...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hayashi RJ,Kanagawa O

    更新日期:2006-05-01 00:00:00

  • Host chemokine (C-C motif) ligand-2 (CCL2) is differentially regulated in HIV type 1 (HIV-1)-infected individuals.

    abstract::Several cytokines and chemokines including chemokine (C-C motif) ligand-2 (CCL2) are induced in HIV-1 infection. However, the impact of HIV-1 viremia on CCL2 regulation is largely unknown. We utilized a DNA oligonucleotide microarray covering 110 inflammatory genes. Five genes were induced by at least 2-fold in PBMCs ...

    journal_title:International immunology

    pub_type: 临床试验,杂志文章


    authors: Ansari AW,Bhatnagar N,Dittrich-Breiholz O,Kracht M,Schmidt RE,Heiken H

    更新日期:2006-10-01 00:00:00

  • DNA vaccination with gp96-peptide fusion proteins induces protection against an intracellular bacterial pathogen.

    abstract::Effective vaccination using in vitro peptide-loaded heat-shock proteins (HSP), tumor-derived HSP and HSP fusion proteins has been shown in viral, parasite and tumor model systems. We demonstrate protective DNA vaccination using gp96-peptide fusion proteins against the intracellular bacterial pathogen Listeria monocyto...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Rapp UK,Kaufmann SH

    更新日期:2004-04-01 00:00:00

  • PD-1 and PD-1 ligands: from discovery to clinical application.

    abstract::Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. Because of the wide range of ligand distribution in the body, its b...

    journal_title:International immunology

    pub_type: 杂志文章,评审


    authors: Okazaki T,Honjo T

    更新日期:2007-07-01 00:00:00

  • Normal D-JH rearranged products of the IgH gene in SCID mouse bone marrow.

    abstract::SCID mice are profoundly immunodeficient, resulting from an inability to carry out the V(D)J recombination reaction during both B cell and T cell development. Recently, however, it was revealed that normal rearrangement frequently did occur in the TCR delta and gamma chain loci in the SCID thymus. To evaluate whether ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Araki R,Itoh M,Hamatani K,Abe M

    更新日期:1996-07-01 00:00:00

  • Schnurri-2 regulates Th2-dependent airway inflammation and airway hyperresponsiveness.

    abstract::Schnurri (Shn)-2 is a large zinc finger-containing protein, which plays a critical role in cell growth, signal transduction and lymphocyte development. In Shn-2-deficient (Shn-2(-/-)) CD4 T cells, the activation of nuclear factor-kappaB is up-regulated and their ability to differentiate into Th2 is enhanced. Here, we ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Iwamura C,Kimura MY,Shinoda K,Endo Y,Hasegawa A,Yamashita M,Nakayama T

    更新日期:2007-06-01 00:00:00

  • Changes in the subsets of CD4+ T cells in Trypanosoma musculi infection: delay of immunological cure in young mice and the weak ability of aged mice to control the infection.

    abstract::After a 3 week course (approximately), during which there is marked lymphoid hyperplasia, Trypanosoma musculi infections in young-adult mice are cured by an immune mechanism involving antibodies of the IgG2a isotype. Both the lymphoid hyperplasia and IgG2a antibody response are T-cell-dependent events and both process...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Utsuyama M,Albright JW,Holmes KL,Hirokawa K,Albright JF

    更新日期:1994-08-01 00:00:00

  • IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.

    abstract::IL-18 is a proinflammatory cytokine that plays an important role in NK cell activation and T(h)1 response. IL-18 has a structural homology to IL-1, particularly IL-1beta. IL-18R, composed of IL-1R-related protein (IL-18Ralpha) and IL-1R accessory protein-like (IL-18Rbeta), belongs to the IL-1R family. Furthermore, IL-...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Tominaga K,Yoshimoto T,Torigoe K,Kurimoto M,Matsui K,Hada T,Okamura H,Nakanishi K

    更新日期:2000-02-01 00:00:00