Abstract:
:Antibody to phosphatidyl choline (PtC) is produced spontaneously in mice, by approximately 2-10% of naturally occurring CD5+ (B1) B cells in the peritoneum. Much of this antibody is encoded by the VH11 gene associated with a specific V kappa 9 gene. Constraints on the size and structure of the H chain CDR3 have been defined from nucleotide sequences of genes expressed by hybridomas and lymphomas derived from adult mice. All employ JH1 and all encode tyrosine as the first amino acid in CDR3, which is either nine or 10 amino acids long; the last six are always the same, start with tyrosine, and are rich in aromatic amino acids. Those with nine amino acids in CDR3 have glycine or serine in the second position and asparagine, serine or proline in the third; those with 10 have an additional aspartate or glycine inserted after the first tyrosine. DSP2 genes are used by 80% and DFL16 by 20%. Productively rearranged VH11 genes in neonates and in 18 day fetal liver display a greater, but still limited degree of diversity. All four JH genes are used and the length of CDR3 varies from three to 12 amino acids, but the first is tyrosine in 58 of 61 and DSP2 genes are used by 80% of these productive VDJ assemblies. Non-productive rearrangements of VH11 in fetal liver show a different pattern; 41% use DFL16 genes and only 40% have a TAx codon in the first position of CDR3. All rearrangements show evidence of a bias in favor of joining the VH11 gene to D genes at positions of matching nucleotide overlap.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Arnold LW,Spencer DH,Clarke SH,Haughton Gdoi
10.1093/intimm/5.11.1365subject
Has Abstractpub_date
1993-11-01 00:00:00pages
1365-73issue
11eissn
0953-8178issn
1460-2377journal_volume
5pub_type
杂志文章abstract::The invertebrate pattern-recognition protein named coelomic cytolytic factor (CCF) and the mammalian cytokine tumor necrosis factor (TNF) share functional analogies that are based on a similar saccharide recognition specificity. In particular, CCF and TNF have been shown to interact with ion channels on the surface of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl100
更新日期:2006-12-01 00:00:00
abstract::T cells of an unique phenotype (CD3+CD4-CD8- alpha beta TCR+) develop in vitro from the hematopoietic progenitors, the majority of which carry homologous alpha beta TCR (V alpha 4J alpha 28/V beta 2D beta 1.1J beta 2.6). In the present study, antigen corresponding to the particular alpha beta TCR was investigated, tak...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1323
更新日期:1994-09-01 00:00:00
abstract::Our aim was to investigate the quantitative relationship between inoculation doses and physiological responses to infection by Salmonella enterica serovar Enteritidis. Rats were orally inoculated with 10-10(9) c.f.u. of S. Enteritidis and monitored for 6 days. Neutrophil and delayed-type hypersensitivity (DTH) respons...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.2.111
更新日期:2002-02-01 00:00:00
abstract::Immunoglobulin (IG:) genes are hypermutated in mature B cells after interaction with antigen and T cells in a germinal center reaction. We and others have recently shown that the human BCL6 gene is also hypermutated in human peripheral blood memory B cells and tonsils. A preliminary analysis of other non-Ig genes (c-M...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.7.1085
更新日期:2000-07-01 00:00:00
abstract::Deletion of alpha i2 subunit of heterotrimeric G proteins induces a 2-4-fold increase in the proportions of CD4 and CD8 single-positive (SP) thymocytes as compared with wild-type littermates, but how G alpha i2 is involved in thymocyte development is unknown. To determine a role for G alpha i2 in a specific developmen...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh204
更新日期:2005-03-01 00:00:00
abstract::A recipient who received a renal graft from a living related donor showed good renal function for as long as 15 years after the withdrawal of azathioprine. The patient's creatinine clearance was maintained at 60 ml/min in spite of the administration of only 10 mg of predonisolon per day. Anti-idiotypic antibodies in t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.5.503
更新日期:1989-01-01 00:00:00
abstract::Peptide-MHC class II complexes (pMHC II) are degraded by MARCH-I-mediated ubiquitination, and the stabilization of pMHC II by loss of its ubiquitination is one phenotype defining the activation of conventional dendritic cells (cDCs). However, the role of such stabilization of pMHC II in the context of T-cell activatio...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt066
更新日期:2014-05-01 00:00:00
abstract::We have searched the human genome for genes that predispose to rheumatoid arthritis (RA) using fluorescence-based microsatellite marker analysis and affected sib-pair linkage study. A panel of 41 Japanese families, each with at least two affected siblings, was typed for genome-wide 358 polymorphic microsatellite marke...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.12.1891
更新日期:1998-12-01 00:00:00
abstract::Pathogenic intracellular bacteria often hijack macrophages for their propagation. The infected macrophages release IL-1β and IL-18 and simultaneously commit suicide, which is called pyroptosis; both responses require caspase-1. Here, we found that pyroptotic cells induced by microbial infection were efficiently engulf...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs161
更新日期:2013-06-01 00:00:00
abstract::Although there has been extensive analysis on the capacity of MHC-peptide tetramers to bind antigen-specific TCR, there have been comparatively few studies regarding the role of the CD4 and CD8 co-receptors in binding and activation by these multimeric molecules. Here, we start from the observation that different anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.1.39
更新日期:2002-01-01 00:00:00
abstract::Oral administration of large doses of protein antigen generally induces a state of systemic unresponsiveness currently termed mucosally induced tolerance. In this study, we used human milk protein (HMP) without casein as a multi-protein antigen for the study of mucosally induced tolerance. The HMP utilized in this stu...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.4.537
更新日期:1998-04-01 00:00:00
abstract::A subclone (160b) of the human B cell (Karpas 160) was shown to produce a novel cytotoxic lymphokine [Factor 2 (F2)] in addition to tumour necrosis factor alpha (TNF-alpha) and beta (TNF-beta). F2 was found to have selective toxicity to numerous human tumour cell lines, particularly the erythroleukaemic cell line K562...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.4.519
更新日期:1992-04-01 00:00:00
abstract::Acute graft-versus-host disease (GvHD) is a serious complication after allogeneic bone marrow transplantation. Donor-derived T cells infiltrate recipient target organs and cause severe tissue damage, often leading to death of the affected patient. Tissue destruction is a direct result of donor CD8+ T cell activation a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.5.503
更新日期:2002-05-01 00:00:00
abstract::Several cis-acting elements regulate the expression of germline transcripts of heavy chain constant region genes and their subsequent switch recombination. To study such elements in the murine gamma1 gene, we have utilized a transgenic approach. In this study we focused on a DNase I hypersensitive site (termed 'Site I...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.12.1705
更新日期:2000-12-01 00:00:00
abstract::Man has been found to produce highly conserved chitinases. The most prominent is the phagocyte-derived chitotriosidase, the plasma levels of which are markedly elevated in some pathological conditions. Here, we report that both polymorphonuclear neutrophils (PMNs) and macrophages (m) are a source of chitotriosidase. T...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh328
更新日期:2005-11-01 00:00:00
abstract::Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A bot...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxv014
更新日期:2015-09-01 00:00:00
abstract::Unlike splenic B cells from normal BALB/c mice, those from autoimmune-prone NZB x NZW (B/W) F1 young mice were hyperresponsive to interleukin 2 (IL2) and proliferated even in the absence of prior stimulation signals. We found that the B cell subpopulation responsible for this event belongs to that with 6B2 (B220)-dull...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.1.99
更新日期:1989-01-01 00:00:00
abstract::The CC chemokine receptor 7 (CCR7) and its two ligands, CCL21 and CCL19, play an important role in migration of immune cells to lymphoid tissue. To analyze the function of CCR7 in T cell immunity to infectious agents in vivo, transgenic (tg) mice expressing CCL21 in an ubiquitous fashion were generated. These mice con...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm098
更新日期:2007-11-01 00:00:00
abstract::Streptococcus suis serotype 2 is an important porcine bacterial pathogen and a zoonotic agent responsible for sudden death, septic shock and meningitis, with exacerbated inflammation being a hallmark of the systemic and central nervous system (CNS) infections. However, S. suis serotype 2 strains are genetically and ph...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz033
更新日期:2019-10-16 00:00:00
abstract::The anti-CD20 chimeric monoclonal antibody rituximab mediates cytotoxicity in malignant B cells via multiple mechanisms, including complement-dependent cytotoxicity (CDC), antibody-dependent cellular cytotoxicity (ADCC) and direct induction of apoptosis. To optimize treatment of non-Hodgkin's lymphoma, a fuller unders...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs048
更新日期:2012-08-01 00:00:00
abstract::CD25(+)CD4(+) regulatory T cells inhibit the activation of autoreactive T cells in vitro and in vivo, and suppress organ-specific autoimmune diseases. The mechanism of CD25(+)CD4(+) T cells in the regulation of experimental autoimmune encephalomyelitis (EAE) is poorly understood. To assess the role of CD25(+)CD4(+) T ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh029
更新日期:2004-02-01 00:00:00
abstract::T-cell immune responses are critical for protection of the host and for disease pathogenesis during infection with Plasmodium species. We examined the regulation of CD4(+) T-cell cytokine responses during infection with Plasmodium berghei ANKA (PbA). CD4(+) T cells from PbA-infected mice produced IFN-γ, IL-4 and IL-10...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq448
更新日期:2010-12-01 00:00:00
abstract::Defects of early complement components (C1, C4 and C2) are associated with the development of systemic lupus erythematosus (SLE). The availability of complement knockout mice has increased our knowledge on the role of complement in the regulation of adaptive immunity. An impaired removal of apoptotic bodies, a disturb...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh159
更新日期:2004-12-01 00:00:00
abstract::Dendritic cells (DCs) are specialized antigen-presenting cells that play pivotal roles in initiating immune responses. However, DC maturation is usually strongly restricted by the stromal microenvironment, especially in non-lymphoid tissues, such as skin and mucosa. Although suppression of DC maturation by stromal cel...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn078
更新日期:2008-09-01 00:00:00
abstract::HLA class II molecules present superantigens more efficiently than their murine counterpart. Therefore, transgenic mice expressing HLA-DQ8 with and without CD28 were used to address the role of CD28 in staphylococcal enterotoxin B (SEB)-driven immune responses. SEB-induced in vitro proliferation of naive DQ8.CD28(-/-)...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf047
更新日期:2002-07-01 00:00:00
abstract::Adhesion molecules of the integrin family are implicated not only in leukocyte migration but also in leukocyte activation. Here we characterize the expression and function of fibronectin receptor integrins on rat mast cells. A rat basophilic leukemia cell line (RBL-2H3) and phorbol ester-stimulated rat peritoneal mast...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.2.251
更新日期:1995-02-01 00:00:00
abstract::Trypanosoma cruzi, the agent of Chagas disease contains a major cysteine proteinase, cruzipain (Cz), with an unusual carboxyl-terminal extension (C-T). We have previously reported the presence of sulfate groups in the N-linked oligosaccharide chains of this domain. In order to evaluate the immune responses to sulfated...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm149
更新日期:2008-04-01 00:00:00
abstract::Fc receptors play important roles for a wide array of immune responses. In contrast to the well-defined Fcγ and Fcε receptors, the molecular and functional characteristics of Fc receptors for IgA and IgM have remained incompletely understood for years. Recent progress has unveiled the characteristics of Fc receptors f...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx059
更新日期:2017-12-30 00:00:00
abstract::Natural killer (NK) cells are a major FcγRIIIA-expressing lymphocyte population that mediate antibody-dependent cellular cytotoxicity. Although NK cells are critical for immunity against viruses and tumors, they are also activated in the joints of patients with rheumatoid arthritis (RA) and may be involved in disease ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz010
更新日期:2019-04-26 00:00:00
abstract::A human neoplastic B cell line SSK41 that expresses IgM on its surface switches spontaneously to IgG-producing cells. The SSK41 line contains a single immunoglobulin heavy-chain locus, the constant region (C) genes of which retain the germline configuration. The IgG-producing SSK41 line was purified by sorting, and sh...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.6.631
更新日期:1989-01-01 00:00:00