Abstract:
:The CC chemokine receptor 7 (CCR7) and its two ligands, CCL21 and CCL19, play an important role in migration of immune cells to lymphoid tissue. To analyze the function of CCR7 in T cell immunity to infectious agents in vivo, transgenic (tg) mice expressing CCL21 in an ubiquitous fashion were generated. These mice contained high amounts of CCL21 in the serum ( approximately 0.3 microg/ml that resulted in CCR7 down-regulation and in a strongly impaired migration of T cells toward CCL21 in vitro. Lymph nodes in CCL21-tg mice were reduced in size but with intact microanatomy and normal distribution of T and B cells. CCL21-tg mice showed a significantly decreased CD8 T cell response to lymphocytic choriomeningitis virus after footpad infection, whereas the response after systemic infection was not altered. Likewise, the CD4 T cell response to footpad infection with Leishmania major was considerably lowered and CCL21-tg mice failed to clear parasites from infected skin. Taken together, these data demonstrate the importance of CCR7 in mediating T cell immunity to viral and parasitic pathogens after local infection.
journal_name
Int Immunoljournal_title
International immunologyauthors
Unsoeld H,Mueller K,Schleicher U,Bogdan C,Zwirner J,Voehringer D,Pircher Hdoi
10.1093/intimm/dxm098subject
Has Abstractpub_date
2007-11-01 00:00:00pages
1281-9issue
11eissn
0953-8178issn
1460-2377pii
dxm098journal_volume
19pub_type
杂志文章abstract::At birth, virtually all peripheral CD8(+) T cells express the CD28 co-stimulatory molecule, but healthy human adults accumulate CD28(-)CD8(+) T cells that often express the CD57 marker. While these CD28(-) subpopulations are known to exert effector-type functions, the generation, maintenance and regulation of CD28(-) ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.8.1327
更新日期:1999-08-01 00:00:00
abstract::We have isolated two cDNA clones encoding the guinea pig receptor for the Fc portion of IgG2 (Fc gamma 2R) from a guinea pig peritoneal macrophage cDNA library. Analysis of the predicted amino acid sequence of the one cDNA clone indicated that the guinea pig Fc gamma 2R is a type I transmembrane protein and has approx...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.9.1335
更新日期:1996-09-01 00:00:00
abstract::Dendritic cells (DCs) are responsible for the initiation of immune responses. Our study demonstrates a new pathway for generating a large quantity of stimulatory monocyte-derived DCs (Mo-DCs) from human monocytes using anti-4-1BB ligand (4-1BBL) mAb to trigger reverse signaling. The anti-4-1BBL-driven Mo-DCs (DCs(alph...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp077
更新日期:2009-10-01 00:00:00
abstract::Responsiveness of dendritic cells (DC) to inflammatory CC chemokines is down-regulated during their maturation. We analyzed the mechanism underlying these events. Cell-surface expression of CC chemokine receptor (CCR)-1, -3 and -5 was increased during differentiation of immature DC (iDC) from monocytes. In contrast, t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.2.167
更新日期:2001-02-01 00:00:00
abstract::Under physiological conditions immune complexes (IC) are efficiently cleared from the circulation and meanwhile provide important feedback signals for the immune system via Fc gamma Rs and complement receptors. Dysregulation of these mechanisms have been implicated in conditions where IC concentrations reach pathologi...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm132
更新日期:2008-02-01 00:00:00
abstract::We have generated a high copy number transgenic mouse line in which expression of mouse IL-7 cDNA is under the control of the mouse MHC class II E alpha promoter. These mice were generated in order to see if IL-7 over-production in the thymus altered either thymocyte differentiation or the process of negative selectio...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.3.401
更新日期:1995-03-01 00:00:00
abstract::The human chymase (HC) is a major granule constituent of mast cells (MCs) residing in the connective tissue and the sub-mucosa. Although many potential substrates have been described for this important MC enzyme, its full range of in vivo substrates has most likely not yet been identified. A major step toward a better...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn128
更新日期:2009-01-01 00:00:00
abstract::The previously defined binding motif of MHC class I H-2Kd-restricted antigenic peptides consists of a Y residue in position P2 and a hydrophobic residue with a large aliphatic side chain (L, I, or V) in position P9/P10 of optimal 9- or 10-mer peptides. We show now that the presence of a charged or a F residue in posit...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.11.1489
更新日期:1993-11-01 00:00:00
abstract::BALB.xid mice, carrying an X-linked mutation leading to the absence of CD5+ B cells, are highly resistant to Trypanosoma cruzi infection. These mice clear blood parasites in the acute phase of infection and do not develop the inflammatory infiltration characteristically observed in the chronic phase of susceptible str...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
abstract::In order to develop predictive markers for a beneficial humoral immune response, we evaluated the in vivo and in vitro response to the pandemic (p)H1N1 vaccine in young and elderly individuals. We measured serum antibody response and associated this with the in vitro B-cell response to the vaccine, measured by activat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxr123
更新日期:2012-03-01 00:00:00
abstract::Recent data suggest that initiation of signal transduction via type 1 Fc epsilon receptor (Fc epsilon RI) and other immunoreceptors is spatially constrained to lipid rafts. In order to better understand the complexity and function of these structures, we prepared mAb against lipid rafts from the rat basophilic leukemi...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.2.213
更新日期:2002-02-01 00:00:00
abstract::Malaria infection induces the production of serum antibodies to a variety of malaria antigens but the prevalence of antibodies to any particular antigen is typically much less than 100%. It has been assumed that non-responsiveness to defined antigens in malaria immune subjects is due to HLS-mediated restriction of the...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.905
更新日期:1996-06-01 00:00:00
abstract::NK cells were first identified in 1975 and represent the prototypical group 1 innate lymphoid cell (ILC). More recently, the discovery of new members of the ILC family has highlighted the complexity of this innate lymphoid lineage. Importantly, it has been recognized that different subsets exist within the group 1 ILC...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxv051
更新日期:2016-01-01 00:00:00
abstract::Human monocytes isolated from peripheral blood of healthy donors show a time-dependent differentiation into macrophages upon in vitro cultivation, closely mimicking their in vivo migration and maturation into extravascular tissues. The mediator(s) of this maturation process has not been yet defined. We investigated th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.7.1075
更新日期:1999-07-01 00:00:00
abstract::The distribution of IgE FcR (Fc epsilon R)-positive and -negative B cells was examined in normal adult mice. Using three-color flow cytometry, the expression of the Fc epsilon R was analyzed on various B-cell subsets present in the peritoneum and spleen. The results demonstrate that in the peritoneal cavity, the Fc ep...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.4.305
更新日期:1991-04-01 00:00:00
abstract::Using Ia antigen-containing lipid vesicles, we investigated by stopped-flow fluorometry the requirements for helper T cell recognition and activation. When azobenzenearsonate-L-tyrosine (ABA-L-Tyr)-specific, I-Ak-restricted helper T cell hybridomas 2-45-12 were mixed with ABA-L-Tyr and purified I-Ak molecules on lipid...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.6.509
更新日期:1990-01-01 00:00:00
abstract::Human CD83 is a cell surface protein expressed predominantly by dendritic cells (DC) and lymphoid cells. So far, there exists no information on the function and distribution of mCD83. Here we demonstrate that mCD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells f...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.9.1347
更新日期:2000-09-01 00:00:00
abstract::Previous evidence from several laboratories suggests that CD8+ T suppressor cells may be important regulatory elements governing specific unresponsiveness of lepromatous lepromatous leprosy patients to M.leprae. To analyse the mechanism of suppression, CD8+ Ts clones were established from lesions and peripheral blood ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.2.121
更新日期:1989-01-01 00:00:00
abstract::We have shown that the dominance of CD8+ T cells expressing TCR Vbeta17 in the adult HLA-A*0201-restricted influenza A/M1(58-66)-specific response is acquired following first antigen exposure. Despite the acquired dominance of Vbeta17+ cells, subdominant M1(58-66)-specific clones expressing non-Vbeta17+ TCR persist in...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.11.1383
更新日期:2001-11-01 00:00:00
abstract::It has been demonstrated that in vivo T cell priming requires CD154-CD40 interaction, which is suggested to be critical in the induction of co-stimulatory activities on antigen-presenting cells (APC). In the current study, we demonstrate that in vivo administration of a high dose of a superantigen, staphylococcal ente...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg080
更新日期:2003-07-01 00:00:00
abstract::Impaired lymphocyte function is a common feature of human diabetes. Nucleoside transport across the plasma membrane is an essential step during lymphocyte growth and activation. In our study, we evaluated the impact of diabetic conditions on nucleoside transport system in B lymphocytes. Examination of the nucleoside t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh195
更新日期:2005-02-01 00:00:00
abstract::Hepatitis C virus (HCV) has the ability to persist in the majority of infected people. Strong, multispecific and sustained T-cell response is correlated with viral clearance. The mechanisms of chronicity by HCV are unclear. HCV could restrain the immune system and establish chronic infection by modulating dendritic ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq033
更新日期:2010-06-01 00:00:00
abstract::Investigation of the process of Peyer's patch (PP) formation has been hampered by difficulties in identifying its initial step in the embryo. In this study, we overcame this problem by means of whole-mount immunohistochemistry using mAb against the molecules which were expressed in the cells accumulated at the site of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.4.507
更新日期:1997-04-01 00:00:00
abstract::CD100/Sema4D belongs to the semaphorin family, factors known to act as repulsive cues for axons during neuronal development. Mouse CD100 plays a crucial role in both humoral and cellular immunity through ligation of the lymphocyte receptor, CD72. It remains controversial, however, whether human CD100 can function thro...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg098
更新日期:2003-08-01 00:00:00
abstract::Adhesion molecules of the integrin family are implicated not only in leukocyte migration but also in leukocyte activation. Here we characterize the expression and function of fibronectin receptor integrins on rat mast cells. A rat basophilic leukemia cell line (RBL-2H3) and phorbol ester-stimulated rat peritoneal mast...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.2.251
更新日期:1995-02-01 00:00:00
abstract::Interleukin-15 (IL-15) is a cytokine critical for maintenance of intestinal intra-epithelial lymphocytes (IELs), especially CD8αα + IELs (CD8αα IELs). In the intestine, IL-15 is produced by intestinal epithelial cells (IECs), blood vascular endothelial cells (BECs) and hematopoietic cells. However, the precise role of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz082
更新日期:2020-05-08 00:00:00
abstract::Seeligeriolysin O (LSO), a member of cholesterol-dependent cytolysins of Listeria seeligeri, exhibits cytokine-inducing activity. In this study, we examined the profile of cytokines expressed in macrophages of mice after stimulation with full-length form of recombinant LSO (rLSO530), C-terminal-truncated protein (rLSO...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh341
更新日期:2005-12-01 00:00:00
abstract::Schnurri (Shn)-2 is a large zinc finger-containing protein, which plays a critical role in cell growth, signal transduction and lymphocyte development. In Shn-2-deficient (Shn-2(-/-)) CD4 T cells, the activation of nuclear factor-kappaB is up-regulated and their ability to differentiate into Th2 is enhanced. Here, we ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm042
更新日期:2007-06-01 00:00:00
abstract::BALB/c mice injected at birth with 10(8) semi-allogeneic (C57BL/6 x BALB.IgHb)F1 spleen cells develop a lupus-like syndrome in which autoantibodies bear exclusively the donor allotype. We have analyzed the evolution of donor B cell chimerism and the autoimmune manifestations during the first year of life in these mice...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.1.67
更新日期:1992-01-01 00:00:00
abstract::Ig are multifunctional molecules with distinct properties assigned to individual domains. To assess the importance of IgM domain assembly in B cell development we generated two transgenic mouse lines with truncated muH chains by homologous integration of the neomycin resistance gene (neo(r)) into exons C(mu)1 and C(mu...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.12.1489
更新日期:2001-12-01 00:00:00