Abstract:
:Germline CH transcripts initiate from a non-coding I exon and terminate downstream of the associated CH exons. Ig heavy chain class switch recombination from the VDJ-C mu gene to particular downstream CH genes appears to be regulated by a process that involves mitogen and/or cytokine induction of germline CH transcripts from the downstream genes. We have examined the expression of germline C mu transcripts (I mu-C mu transcripts) in splenic B cells and pre-B cell lines after cytokine and mitogen stimulation. In contrast to the expression of the germline transcripts from downstream CH genes, expression of germline C mu transcripts was constitutive and unaffected by mitogen and cytokine treatment. After a primary switch recombination event, the germline I mu promoter, which is now associated with a downstream CH gene, continues to be active--leading to the generation of a novel germline transcript consisting of the I mu exon spliced to the CH exons of the switched CH gene. We discuss the potential role of the expression of hybrid I mu-containing transcripts in the class switch process. We also describe a novel and sensitive assay, based on the detection of the hybrid I mu-containing transcripts, that allows detection of class switch recombination events even in heterogeneous populations of cells.
journal_name
Int Immunoljournal_title
International immunologyauthors
Li SC,Rothman PB,Zhang J,Chan C,Hirsh D,Alt FWdoi
10.1093/intimm/6.4.491subject
Has Abstractpub_date
1994-04-01 00:00:00pages
491-7issue
4eissn
0953-8178issn
1460-2377journal_volume
6pub_type
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journal_title:International immunology
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更新日期:1997-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/6.11.1671
更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/intimm/8.2.159
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh233
更新日期:2005-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.3.369
更新日期:1995-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh291
更新日期:2005-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.5.619
更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/dxn003
更新日期:2008-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/6.10.1585
更新日期:1994-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf025
更新日期:2002-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm109
更新日期:2007-12-01 00:00:00
abstract::We have shown that the dominance of CD8+ T cells expressing TCR Vbeta17 in the adult HLA-A*0201-restricted influenza A/M1(58-66)-specific response is acquired following first antigen exposure. Despite the acquired dominance of Vbeta17+ cells, subdominant M1(58-66)-specific clones expressing non-Vbeta17+ TCR persist in...
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pub_type: 杂志文章
doi:10.1093/intimm/13.11.1383
更新日期:2001-11-01 00:00:00
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更新日期:2001-09-01 00:00:00
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更新日期:2009-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/3.7.673
更新日期:1991-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp082
更新日期:2009-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn035
更新日期:2008-06-01 00:00:00
abstract::Chronic viral infections are often characterized by CD8 T-cell responses with poor cytokine secretion potential and limited expansion of the CD8 T-cell pool, collectively referred to as CD8 T-cell exhaustion. Exhaustion of lymphocytic choriomeningitis virus (LCMV)-specific CD8 T cells was shown to be partially regulat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp107
更新日期:2010-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
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更新日期:1995-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2001-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/8.6.905
更新日期:1996-06-01 00:00:00