Abstract:
:cDNA clones which strongly hybridized with a 3.1 kb mRNA from mouse macrophages and macrophage cell lines and weakly with mRNA from P815 but not from a variety of other cell lines and tissues were isolated from cDNA libraries constructed using mRNA from murine macrophage cell lines and peritoneal macrophages. Treatment of a macrophage cell line with macrophage stimulators significantly enhanced transcription of the mRNA. Sequencing analysis of these clones demonstrated that the cDNA consisted of 3036 bp insert containing a 2478 bp open reading frame followed by a 538 bp 3' untranslated region. The amino acid sequence, deduced from the nucleotide sequence of the cDNA, predicted a protein containing a signal peptide, an extracellular region, a transmembrane domain, and a cytoplasmic tail. The extracellular region had five putative N-glycosylation sites and a cysteine-rich domain, whereas the cytoplasmic region consisted of a proline-rich amino acid sequence significantly similar to CD2. SDS-PAGE and NEPHGE SDS-PAGE analysis of the immunoprecipitated membrane of the macrophage cell lines prepared by using rabbit anti-MS2 peptide antibody raised against a synthetic peptide preparation relative to a hydrophilic region of the MS2 amino acid sequence confirmed that MS2 protein is a cross-linked protein having approximate molecular sizes of 89 kd and pl 6.5-7.0. These results show that MS2 protein is a novel cell surface antigen expressed mainly in monocytic lineages.
journal_name
Int Immunoljournal_title
International immunologyauthors
Yoshida S,Setoguchi M,Higuchi Y,Akizuki S,Yamamoto Sdoi
10.1093/intimm/2.6.585subject
Has Abstractpub_date
1990-01-01 00:00:00pages
585-91issue
6eissn
0953-8178issn
1460-2377journal_volume
2pub_type
杂志文章abstract::The dynamic behaviour of isolated populations of peritoneal LPS-reactive (LPSr) B lymphocytes was studied upon transfer of peritoneal cells (PerC) from C57BL/6 LPS responder into C57BL/10ScCr LPS non-responder mice. We have followed the persistence and life-span of the transferred LPSr donor B cells in the spleen and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.1.73
更新日期:1990-01-01 00:00:00
abstract::The invertebrate pattern-recognition protein named coelomic cytolytic factor (CCF) and the mammalian cytokine tumor necrosis factor (TNF) share functional analogies that are based on a similar saccharide recognition specificity. In particular, CCF and TNF have been shown to interact with ion channels on the surface of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl100
更新日期:2006-12-01 00:00:00
abstract::To investigate the involvement of retinoic acid receptor (RAR)-alpha in allograft rejection, we investigated the effect of a novel selective agonist to the receptor, ER-38925, in a mouse cardiac allograft model. Prophylactic treatment with ER-38925 inhibited the acute rejection of the mouse cardiac allograft (BALB/c -...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh066
更新日期:2004-05-01 00:00:00
abstract::Natural killer (NK) cells are a major FcγRIIIA-expressing lymphocyte population that mediate antibody-dependent cellular cytotoxicity. Although NK cells are critical for immunity against viruses and tumors, they are also activated in the joints of patients with rheumatoid arthritis (RA) and may be involved in disease ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz010
更新日期:2019-04-26 00:00:00
abstract::Under equilibrium conditions, the affinities of five anti-IgG2a mAb isolated from virus-infected mice were comparable to other high-affinity auto-antibodies. Similar to rheumatoid factors, these anti-IgG2a auto-antibodies bound to aggregated or complexed IgG2a with 50 to 1500-fold higher avidity than their monomeric c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1125
更新日期:1995-07-01 00:00:00
abstract::The mucosa is one of the initial sites of group A streptococcal (GAS) infection and salivary IgA (sIgA) is thought to be critical to immunity. However, the target epitopes of sIgA and the function of sIgA in GAS immunity, in particular the role of accessory cells and complement, is largely unknown. We studied the aqui...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.4.569
更新日期:1999-04-01 00:00:00
abstract::SKG mice, a newly established model of rheumatoid arthritis (RA), spontaneously develop autoimmune arthritis accompanying extra-articular manifestations, such as interstitial pneumonitis. To examine possible roles of T cells for mediating this systemic autoimmunity, we generated T cell clones from arthritic joints of ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn091
更新日期:2008-10-01 00:00:00
abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.11.1605
更新日期:2000-11-01 00:00:00
abstract::The immediate responses to aerosolized staphylococcal enterotoxin B (SEB) in respiratory toxic shock were studied in the circulation of rhesus monkeys with low antibody levels following immunization with SEB toxoid-containing microspheres. Both the surviving and dying monkeys had toxic shock syndrome 4-48 h after SEB ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.12.1825
更新日期:1997-12-01 00:00:00
abstract::Mice with a targeted deletion of the T-bet gene exhibit spontaneous airway hyperresponsiveness (AHR), airway inflammation, enhanced recovery of T(h)2 cytokines from bronchoalveolar lavage fluid, sub-epithelial collagen deposition and myofibroblast transformation. Here we analyze the mechanisms responsible for the chro...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh281
更新日期:2005-08-01 00:00:00
abstract::We have shown that the dominance of CD8+ T cells expressing TCR Vbeta17 in the adult HLA-A*0201-restricted influenza A/M1(58-66)-specific response is acquired following first antigen exposure. Despite the acquired dominance of Vbeta17+ cells, subdominant M1(58-66)-specific clones expressing non-Vbeta17+ TCR persist in...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.11.1383
更新日期:2001-11-01 00:00:00
abstract::CD18-deficient PL/J mice develop dermatitis characterized by hyperkeratosis, and a mixed dermal and epidermal inflammatory infiltrate. The development of this disease requires low-level CD18 expression and at least two PL/J loci. Currently, the mechanisms by which decreased beta(2) integrin expression on leukocytes pr...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh028
更新日期:2004-02-01 00:00:00
abstract::The class II trans-activator (CIITA) is a bi- or multi-functional domain protein which plays a critical role in the expression of MHC class II genes. We report that removal of the N-terminal 151 amino acids, encompassing all of the acidic domain but leaving intact the proline/serine/threonine-rich domain, results in a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1545
更新日期:1997-10-01 00:00:00
abstract::IFN-regulatory factor (IRF)-4 is a member of the IRF family of transcription factors expressed in lymphocytes and macrophages. The previous studies using mice deficient in the IRF-4 gene showed profound defects in function of both B and T cells. To further investigate the role of IRF-4 in CD4(+) T cell function, IRF-4...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg001
更新日期:2003-01-01 00:00:00
abstract::Lymphocyte migration from the blood into specific tissues is directed by their expression of adhesion molecules referred to as homing receptors. The homing receptor L-selectin, for example, directs the migration of lymphocytes into peripheral lymph nodes (PLN). Since bovine gamma delta T cells, a major lymphocyte subs...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.1.81
更新日期:1994-01-01 00:00:00
abstract::MicroRNAs 125a and 125b are predicted to be able to bind to the B lymphocyte-induced maturation protein-1 (BLIMP-1) and IFN regulatory protein-4 (IRF-4) transcription factors, which are essential for plasma cell differentiation. A computational survey of the human and mouse genomes revealed that miR-125a and miR-125b ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq042
更新日期:2010-07-01 00:00:00
abstract::J11d is expressed on a variety of hemopoietic cells, including B cells. In previous studies, we have shown that B cells are heterogeneous with respect to their levels of J11d expression and that memory B cells are contained within the J11dlo B cell population. In this report, we have further examined the relationship ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.6.691
更新日期:1992-06-01 00:00:00
abstract::The transcription factor interferon regulatory factor-5 (IRF5) plays an important role in innate immune responses via the TLR-MyD88 (Toll-like receptor - myeloid differentiation primary response 88) pathway. IRF5 is also involved in the pathogenesis of the autoimmune disease systemic lupus erythematosus (SLE). Recent ...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy032
更新日期:2018-10-29 00:00:00
abstract::Metazoan species, from sponges to insects and mammals, possess successful defence systems against their pathogens and parasites. The evolutionary origins of these diverse systems are beginning to be more comprehensively investigated and mapped out. We have collected 1811 metazoan immunity genes from literature and gen...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm109
更新日期:2007-12-01 00:00:00
abstract::Rheumatic fever (RF) follows a throat infection with different M-serotypes of beta-hemolytic group A streptococci (GAS) and can affect different tissues, predominantly the heart. It is thought to be an autoimmune illness. Although histological examination of affected heart shows an infiltrate consisting mainly of T ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1235
更新日期:1994-08-01 00:00:00
abstract::T lymphocyte responses to the MHC of an evolutionarily distant species are known to be weak compared with responses against allogeneic MHC products within a species. This fact was used to examine the regions of human MHC class II molecules required for the stimulation of strong primary immune responses against MHC all...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.8.935
更新日期:1992-08-01 00:00:00
abstract::The mature dendritic cell (DC) is considered to be the most potent antigen-presenting cell. Regulation of the DC, particularly its survival, is therefore critical. Mature DC are markedly more sensitive to HLA-DR-mediated apoptosis than immature DC. To further characterize this key survival difference, we compared the ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf058
更新日期:2002-08-01 00:00:00
abstract::Murine complement receptor type 2 (MCR2) is homologous to human CR2, whereas murine CR1 (MCR1) is structurally and evolutionary different from human CR1. Since ligand specificities of MCR1 and MCR2 are not completely clarified, we analyzed their functional characteristics to correlate them with structural information ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.4.337
更新日期:1993-04-01 00:00:00
abstract::The thymus leukemia antigens (TL) belong to the MHC class Ib family and can be recognized by CD8-dependent or -independent cytotoxic T lymphocytes (CTL) showing TL, but not H-2, restriction. We previously reported that the CTL epitope is TAP independent and in the present study we further characterize the recognition ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg131
更新日期:2003-11-01 00:00:00
abstract::It is increasingly evident that neutrophils are able to cross-talk with other leukocytes to shape ongoing inflammatory and immune responses. In this study, we analyzed whether human NK cells may influence the survival and activation of neutrophils under co-culture conditions. We report that NK cells exposed to either ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq434
更新日期:2010-10-01 00:00:00
abstract::Streptococcus suis capsular type 2 is an important swine pathogen and an agent of zoonosis. Although meningitis is the most common form of disease, septicemia and septic shock are also frequently reported. Despite reports that CD14 is involved in the recognition of encapsulated S. suis by host cells, the mechanisms un...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm003
更新日期:2007-04-01 00:00:00
abstract::P-selectin is a Ca(2+)-dependent lectin that participates in leukocyte adhesion to vascular endothelium and platelets. Myeloid cells and a subset of T lymphocytes express carbohydrate ligands at the cell surface. Previously, we suggested that heat stable antigen (HSA/mouse CD24), an extensively glycosylated cell surfa...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.10.1557
更新日期:1995-10-01 00:00:00
abstract::Human dendritic cells (DC) generated from CD34+ hematopoietic progenitors cultured in the presence of granulocyte macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha are related to Langerhans cells (DLC) and have been shown to induce a strong proliferation of allogeneic CD4+ T cells. Th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1177
更新日期:1994-08-01 00:00:00
abstract::Organs of transgenic pigs that express human complement regulatory proteins are under assessment as an alternative to transplantation. A major barrier to the transplantation of pig organs is the hyperacute rejection caused by pre-existing antibodies and complement. Pig cells are very susceptible to human complement, p...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.6.869
更新日期:1997-06-01 00:00:00
abstract::In contrast to most organs, the anatomy of the liver may allow naive CD8(+) T cells to make direct contact with liver parenchymal cells. We have previously shown, using a combination of TCR transgenic T cells specific for H-2 K(b) and hepatocytes expressing a transgenic H-2 K(b) molecule, that hepatocytes can induce a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.8.1225
更新日期:1999-08-01 00:00:00