Abstract:
:Inadequate co-stimulation of tumor reactive T cells may contribute to the fact that antigenic tumors are not normally rejected by the immune system. We recently reported that the induction of profound unresponsiveness in a T cell clone by melanoma cells expressing MHC class II antigens may provide a mechanism for these tumor cells to escape immunosurveillance. Here we demonstrate that two T cell clones (sTC3 and sTS5) produced high amounts of IL-10 after being rendered anergic by autologous melanoma cells. Co-culture of these T cell clones with melanoma cell transfectants expressing B7, which failed to induce anergy, resulted in a significantly lower production of IL-10. IL-10 production by the anergic T cell clones correlated with an impaired ability to produce IL-2 in response to TCR mediated activation. Neutralization of IL-10 reduced the duration of T cell unresponsiveness from 14 to only 4 days, but inhibition of IL-10 production during initiation of anergy showed no effect on it. Induction of the unresponsive state, as well as the subsequent IL-10 production in sTC3 cells, could be prevented by the addition of cyclosporin A to the primary co-culture of sTC3 and the autologous melanoma. Taken together, these results indicate that IL-10 is important for maintenance of T cell anergy induced by contact with nonprofessional antigen presenting cells such as MHC class II+ melanoma cells. Furthermore, we were able to detect IL-10 in serum from melanoma patients and IL-10 mRNA in tumor infiltrating lymphocytes isolated from melanoma metastases, suggesting an in vivo relevance of our in vitro findings.
journal_name
Int Immunoljournal_title
International immunologyauthors
Becker JC,Czerny C,Bröcker EBdoi
10.1093/intimm/6.10.1605subject
Has Abstractpub_date
1994-10-01 00:00:00pages
1605-12issue
10eissn
0953-8178issn
1460-2377journal_volume
6pub_type
杂志文章abstract::Human endothelial cells (ECs) constitutively express OX40L and co-stimulate memory CD4(+) T cell proliferation that is dependent upon OX40-OX40L interaction. In vivo, OX40 prolongs T cell survival; however, an unanswered question is whether it can also prolong synthesis of proliferation-sustaining cytokines such as IL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh255
更新日期:2005-06-01 00:00:00
abstract::In this study, we show that IFNalpha increases the chemotaxis of human B cells to CCL20, CCL21 and CXCL12 in a dose- and time-dependent manner. The effect was maximal with 2000 IU ml(-1) IFNalpha. It peaked at 24 h and decreased thereafter. At 24 h, IFNalpha had increased B-cell chemotaxis to CCL20 by 20 +/- 6.2% (n =...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh227
更新日期:2005-04-01 00:00:00
abstract::Lipid mediators, such as prostanoids and leukotrienes (LTs), exert a range of actions through their own receptors on cell surfaces in various pathophysiological conditions. It has been reported that the production of prostanoids and LTs is significantly elevated in the skin lesions of some chronic inflammatory skin di...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy087
更新日期:2019-08-23 00:00:00
abstract::Dendritic cells (DCs) are responsible for the initiation of immune responses. Our study demonstrates a new pathway for generating a large quantity of stimulatory monocyte-derived DCs (Mo-DCs) from human monocytes using anti-4-1BB ligand (4-1BBL) mAb to trigger reverse signaling. The anti-4-1BBL-driven Mo-DCs (DCs(alph...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp077
更新日期:2009-10-01 00:00:00
abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.11.1605
更新日期:2000-11-01 00:00:00
abstract::Regulatory T cells (Tregs), which could be down-regulated by IL-28B, were reported to suppress T-cell-mediated immunity. The aim of this study was to investigate the role of IL-28B on the immune responses and protective efficacy of a tuberculosis (TB) subunit vaccine. First, a recombinant adenoviral vector expressing ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxv061
更新日期:2016-02-01 00:00:00
abstract::Src homology region 2-domain-containing phosphatase-1 (SHP-1) plays an important role in the regulation of signaling from various receptors in hematopoietic cells. In mast cells, SHP-1 has been shown to negatively regulate the initial signaling triggered by high-affinity receptor for IgE (FcepsilonRI) and positively r...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm070
更新日期:2007-09-01 00:00:00
abstract::Previous evidence from several laboratories suggests that CD8+ T suppressor cells may be important regulatory elements governing specific unresponsiveness of lepromatous lepromatous leprosy patients to M.leprae. To analyse the mechanism of suppression, CD8+ Ts clones were established from lesions and peripheral blood ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.2.121
更新日期:1989-01-01 00:00:00
abstract::HLA class II molecules present superantigens more efficiently than their murine counterpart. Therefore, transgenic mice expressing HLA-DQ8 with and without CD28 were used to address the role of CD28 in staphylococcal enterotoxin B (SEB)-driven immune responses. SEB-induced in vitro proliferation of naive DQ8.CD28(-/-)...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf047
更新日期:2002-07-01 00:00:00
abstract::The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle control or cellular response to...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf060
更新日期:2002-08-01 00:00:00
abstract::Eye-derived antigen-presenting cells (APCs) are known to contribute to the immune privilege status of the eye by inducing a form of peripheral tolerance that deviates T(h)1 type of pro-inflammatory immune responses. Similar systemic tolerance can also be induced by non-ocular APCs exposed to transforming growth factor...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl006
更新日期:2006-05-01 00:00:00
abstract::Toll-like receptors (TLRs) recognize a variety of microbial products and induce defense responses. Pathogen sensing by TLRs occurs either on the cell surface or in endolysosomes. TLR-dependent responses are greatly influenced by the site of pathogen sensing. TLR heterodimers TLR1/TLR2 and TLR2/TLR6 recognize tri- or d...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu054
更新日期:2014-10-01 00:00:00
abstract::IL-7 is a required factor for T-cell homeostasis. Because of low expression levels and poor reagent availability, the cellular sources of IL-7 have proven challenging to characterize. In this study, we describe a reporter mouse in which enhanced GFP is expressed from the endogenous Il7 locus. We show that IL-7 is prod...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt012
更新日期:2013-08-01 00:00:00
abstract::A subclone (160b) of the human B cell (Karpas 160) was shown to produce a novel cytotoxic lymphokine [Factor 2 (F2)] in addition to tumour necrosis factor alpha (TNF-alpha) and beta (TNF-beta). F2 was found to have selective toxicity to numerous human tumour cell lines, particularly the erythroleukaemic cell line K562...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.4.519
更新日期:1992-04-01 00:00:00
abstract::Soluble recombinant (r) P-selectin and rP-selectin immobilized on plastic surfaces were tested for their capacity to activate neutrophils to produce superoxide anion. Soluble rP-selectin was incapable of activating leukocytes, whereas immobilized rP-selectin was able to induce leukocyte activation. When neutrophils we...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.4.487
更新日期:2000-04-01 00:00:00
abstract::In vaccine development, a major objective is to induce strong, specific T cell responses. This might be obtained by targeting antigen to cell surface molecules that efficiently channel the antigen into endocytic compartments for loading of MHC molecules. Antibodies have been used to deliver antigen; however, it is imp...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf110
更新日期:2002-12-01 00:00:00
abstract::CD70 and CD80 are co-stimulatory molecules which belong to the tumor necrosis factor family and the B7 family respectively. When they are co-expressed by gene-modified TS/A tumor cells, they provide an efficient protective and long-lasting T-dependent antitumor response. We first showed that when CD70 and CD80 were de...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg038
更新日期:2003-03-01 00:00:00
abstract::Epitope spreading has been best characterized as an exacerbating factor in CD4(+) T cell-dependent autoimmune disease models and is believed to occur via presentation of antigens liberated by tissue destruction initiated by CD4(+) T cells specific for a primary epitope. The growing evidence that exogenous antigens can...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.5.625
更新日期:2001-05-01 00:00:00
abstract::Chronic viral infections are often characterized by CD8 T-cell responses with poor cytokine secretion potential and limited expansion of the CD8 T-cell pool, collectively referred to as CD8 T-cell exhaustion. Exhaustion of lymphocytic choriomeningitis virus (LCMV)-specific CD8 T cells was shown to be partially regulat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp107
更新日期:2010-01-01 00:00:00
abstract::Dendritic cells (DC) are key players in the initiation and modulation of adaptive immune responses due to their ability to acquire and present antigen and stimulate T cells. For the induction of effector T cell functions, antigen must be presented by activated DC. In this study, we have compared uptake of antigen by m...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp053
更新日期:2009-07-01 00:00:00
abstract::Blocking T cell co-stimulatory signals by anti-B7-1/B7-2 mAb is an attractive approach to treat autoimmune diseases. However, anti-B7-1/B7-2 mAb treatment is known to exacerbate autoimmune diseases through mechanisms not fully understood. Tumor necrosis factor alpha (TNF-alpha) and reactive oxygen species (ROS) also p...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm012
更新日期:2007-04-01 00:00:00
abstract::Mouse CD4+ T cells were subdivided into two subpopulations, naive (CD44low CD45RBhigh) and memory (CD44high CD45RBlow) T cells, by flow cytometric analysis. Examination of spleen and peripheral blood of C57BL/6 mice of various ages revealed that there was a reciprocal age-associated change in these two subpopulations,...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.1.97
更新日期:1995-01-01 00:00:00
abstract::Human CD83 is a cell surface protein expressed predominantly by dendritic cells (DC) and lymphoid cells. So far, there exists no information on the function and distribution of mCD83. Here we demonstrate that mCD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells f...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.9.1347
更新日期:2000-09-01 00:00:00
abstract::The course and severity of lupus in spontaneous murine lupus models varies among laboratories, which may be due to variations in diet, housing and/or local environmental conditions. In this study, we investigated the influence of common rodent diets while keeping other factors constant. Female lupus-prone MRL/lpr (MRL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxx033
更新日期:2017-06-01 00:00:00
abstract::The specificity of self MHC-restricted T cells responding to peptide fragments of processed antigen has been well characterized. However, the means by which alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We now provide evidence that the recognition of class I human leukocyte antigen (HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.1.103
更新日期:1993-01-01 00:00:00
abstract::Despite intense recent interest, the suppressive mechanisms of regulatory CD25+CD4 T cells remain poorly understood. One deficiency in the field is the lack of in vivo models where the effects of regulatory CD25+CD4 T cells on antigen-specific responder T cells can be measured quantitatively. We describe one such mode...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh213
更新日期:2005-04-01 00:00:00
abstract::CD161(++)IL-18Rα(+)CD8(+) human T cells have recently been identified as a new subset of memory cells but their exact role remains unclear. CD161(++)IL-18Rα(+)CD8(+), mucosal-associated invariant T cells express a semi-invariant TCR Vα7.2-Jα33, which recognizes the MHC-related protein 1. On the basis of properties inc...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs069
更新日期:2012-10-01 00:00:00
abstract::Our aim was to investigate the quantitative relationship between inoculation doses and physiological responses to infection by Salmonella enterica serovar Enteritidis. Rats were orally inoculated with 10-10(9) c.f.u. of S. Enteritidis and monitored for 6 days. Neutrophil and delayed-type hypersensitivity (DTH) respons...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.2.111
更新日期:2002-02-01 00:00:00
abstract::O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1409
更新日期:1994-09-01 00:00:00
abstract::The mature dendritic cell (DC) is considered to be the most potent antigen-presenting cell. Regulation of the DC, particularly its survival, is therefore critical. Mature DC are markedly more sensitive to HLA-DR-mediated apoptosis than immature DC. To further characterize this key survival difference, we compared the ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf058
更新日期:2002-08-01 00:00:00