Abstract：
:The enzymatic and regulatory properties of Ca2+/calmodulin-dependent protein kinase in the postsynaptic density (mPSDp CaM kinase) of the rat forebrain was compared with those of soluble Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). mPSDp CaM kinase was different from soluble CaM kinase II in terms of substrate specificity, regulatory consequences and sites of autophosphorylation. Both soluble and PSD kinases generated Ca(2+)-independent activity by autophosphorylation and Ca(2+)-independent activity almost reached the maximum during the first minute of autophosphorylation. Ca(2+)-independent activity of mPSDp CaM kinase was more stable than that of the soluble kinase under autophosphorylating conditions. Autophosphorylation of the kinases decreased the mobility of the kinases on SDS-polyacrylamide gels. The mobility shift and determination of 32P phosphate incorporation into the kinases demonstrated that there were three species in mPSDp CaM kinase alpha isoform: two active forms with and without the mobility shift (about 22 and 19%, respectively), and an inactive form (about 59%). However, there was only one species in the soluble kinase alpha isoform, which was active. The maximum incorporation of 32P phosphate into mPSDp CaM kinase alpha isoform was less than that of the soluble kinase. Tryptic peptide analysis indicated that the phosphorylation sites of mPSDp CaM kinase alpha isoform differed from those of the soluble kinase.
journal_name
Brain Resjournal_title
Brain researchauthors
Ochiishi T,Sugiura H,Yamauchi Tdoi
10.1016/0006-8993(93)91222-esubject
Has Abstractpub_date
1993-04-30 00:00:00pages
97-107issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(93)91222-Ejournal_volume
610pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Gulf War Illness (GWI) is a chronic disease that affects the 1991 Gulf War (GW) veterans for which treatment is lacking. It has been hypothesized that drugs used to protect military personnel from chemical attacks and insects during the war: pyridostigmine bromide (PB),N, N-diethyl-m-toluamide (DEET), and permethrin (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.12.030
更新日期：2018-02-15 00:00:00
abstract：:Nerve growth factor (NGF) treatment, given as a single 200 BU intraocular injection at the time of optic nerve transection, was found to significantly accelerate the retinal ganglion cell response to axotomy in the newt (Notophthalmus viridescens). In the control series the per cent of neurons in the retinal ganglion ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90327-5
更新日期：1979-08-03 00:00:00
abstract：:To determine if aging in rats is associated with insensitivity of cerebral tissue to thyroid hormones (TH), the expression of a TH responsive protein or (THRP) in cerebral tissue was studied in male Fischer rats at 4, 12 and 24 months of age during euthyroid, hypothyroid and hyperthyroid states. The basal levels of TH...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00144-9
更新日期：1998-05-18 00:00:00
abstract：:Interaural intensity and time differences (IID and ITD) are two binaural auditory cues for localizing sounds in space. This study investigated the spatio-temporal brain mechanisms for processing and integrating IID and ITD cues in humans. Auditory-evoked potentials were recorded, while subjects passively listened to n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.03.095
更新日期：2006-05-30 00:00:00
abstract：:This study has presented cells, fibers and non-neuronal elements as seen in 3 dimensions using the SEM. Our discussion on possible artifacts which can occur with this method is offered in the hope that others will develop better techniques of dissection and specimen preparation. With the picture of normal tissue in mi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-0173(84)90006-7
更新日期：1984-12-01 00:00:00
abstract：:In the brain, nitric oxide (NO) has been identified as a messenger molecule and a mediator of excitatory amino acid-induced neurotoxicity. In this study, the effects of NO on serum-induced mitogenesis and cell proliferation of the cerebellar glial cells were assessed. NO-generating agent, S-nitroso-N-acetylpenicillami...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91678-8
更新日期：1992-10-02 00:00:00
abstract：:Concentrations of monoamines and metabolites and uric acid were measured in sequential 15-min microdialysis samples taken from the area of the supraoptic nucleus (SON) in 9 urethane-anaesthetized rats. A decrease in blood pressure of 40 mm Hg was induced by withdrawing 3-4 ml of blood from an indwelling jugular cathet...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91016-5
更新日期：1988-02-09 00:00:00
abstract：:The effect of lactic acidosis on calcium influx, accumulation and efflux was studied in primary cultures of neonatal cortical rat astrocytes. Treatment of cultures with 20 mM sodium lactate, pH 6.0, for 10-60 min resulted in a 35% reduction of 45Ca2+ influx. The decrease in calcium influx was pH dependent because a si...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90371-s
更新日期：1993-02-26 00:00:00
abstract：:Previous studies showed that hyperbaric oxygen (HBO) promoted cell proliferation in hypoxic-ischemic (HI) neonate rats. Neural stem cells (NSC) existed in the brain lifelong and can be activated. This study was undertaken to assess whether HBO treatment promoted the proliferation of NSC and repaired the brain damage r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.05.016
更新日期：2008-07-30 00:00:00
abstract：:Previous studies have shown that one of the genetic variants BDNF val66met polymorphism is associated with drug addiction. Age at onset for drugs abuse has also been regularly cited as a factor associated with high genetic predispositions. We conducted an exploratory research to investigate the association of BDNF val...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.07.022
更新日期：2010-09-24 00:00:00
abstract：:Rats were administered haloperidol (0.5 mg/kg/day) for 21 days. Twenty percent of locus coeruleus (LC) neurons recorded were not spontaneously active, while no such neuron was observed in control animals. A further 22% of neurons (spontaneously active) had rates greater than 2 standard deviations from the mean of LC c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90346-4
更新日期：1985-01-28 00:00:00
abstract：:Neurons immunoreactive for the calcium-binding protein calretinin (CR) were visualized in the substantia nigra (SN) pars compacta (SNc), reticulata (SNr) and lateralis (SNl), the ventral tegmental area (VTA) and the subthalamic nucleus (STN) of squirrel monkeys. The density of CR perikarya was high in VTA, moderate in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01374-1
更新日期：1996-02-05 00:00:00
abstract：:The location and nature of the changes underlying long-term potentiation (LTP) remain controversial issues. In this study, we tested the possibility that changes in binding properties of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA/quisqualate and N-methyl-D-aspartate (NMDA) subtype of glutamate rec...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90767-4
更新日期：1992-02-28 00:00:00
abstract：:Transgenic mice expressing human APPswe and PS1-A264E mutations mimic certain neuropathological features of Alzheimer's disease (AD). These mice have elevated levels of the highly fibrillogenic amyloid beta1-42 peptide (Abeta42) and develop amyloid plaques around the age of 9 months. Our aim was to find whether these ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02617-3
更新日期：2002-07-12 00:00:00
abstract：:Endothelin-1 (ET-1) has been implicated in hematoma-induced cerebral vasoconstriction and modification of cerebral microvascular reactivity, particularly attenuation of vasodilation to cAMP-dependent dilators and enhanced vasoconstriction to ET-1. We examined effects of the ET-1 antagonist, BQ-123, on hematoma-induced...
journal_title：Brain research
pub_type： 杂志文章
doi：
更新日期：1996-09-23 00:00:00
abstract：:Angiotensin peptides are thought to act as neurotransmitters or neuromodulators in central osmoregulation. We tested the hypothesis that angiotensin peptides are released in the paraventricular nucleus (PVN) of the hypothalamus upon local osmotic stimulation. Brain microdialysis and radioimmunoassay (RIA) techniques w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91215-7
更新日期：1994-02-21 00:00:00
abstract：:Calcium-binding proteins play a critical role in vertebrate sensory cells, and some of them have been detected in mechanosensory and chemosensory cells of bony and cartilaginous fishes. In this study immunohistochemistry and Western blot were used to investigate the occurrence and the distribution of S100 protein and ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.05.070
更新日期：2007-08-08 00:00:00
abstract：:5-Hydroxytryptamine (5-HT)-like immunoreactive neurones were studied during the development of myenteric plexus explant cultures from the guinea-pig taenia coli over a period of 3 weeks in vitro, using immunofluorescence histochemistry. Brightly fluorescent 5-HT-like immunoreactive neuronal cell bodies and fibres were...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90865-5
更新日期：1984-06-18 00:00:00
abstract：:Temperature dysregulation is well known in generalized convulsive status epilepticus but so far has not been reported in non-convulsive forms. In order to detect possible subtle alterations, we have analyzed the capability to compensate for external cooling in an animal model of limbic status epilepticus. Rats with el...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.10.034
更新日期：2007-01-05 00:00:00
abstract：:Dendritic architecture and distribution of dendritic protrusions were studied on Golgi-impregnated neurons in the medial preoptic area (MPOA) of infant rats (7 and 20 days old), of animals at puberty (34 days old) and of postpubertal rats (90 days old) using computerized image analysis. The protrusions showed a peak d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90006-4
更新日期：1986-04-01 00:00:00
abstract：:The concentration of extracellular Ca2+ has been shown to enhance or attenuate [3H]acetylcholine (ACh) release subsequent to a conditioning stimulus in rat brain hippocampal slices. Slices were incubated in vitro in [3H]choline solution. Subsequently the slices were subjected to two consecutive electrical stimulations...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90261-7
更新日期：1987-09-08 00:00:00
abstract：:The deposition of amyloid beta-peptide (Abeta) plays a crucial role in the pathogenesis of Alzheimer's disease (AD). Beta-site APP cleaving enzyme (BACE) is the rate-limiting enzyme in the Abeta formation. BACE mutations/polymorphisms may be associated with AD. We searched the BACE coding region mutations/polymorphism...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03849-0
更新日期：2003-01-24 00:00:00
abstract：:The melanocortins (MC) can affect interscapular brown adipose tissue (IBAT) thermogenesis via its sympathetic nervous system (SNS) innervation. We chose a site of high MC4-receptor (MC4-R) mRNA co-localization with SNS outflow neurons to IBAT, the subzona incerta (subZI) to test whether IBAT thermogenesis could be inc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.07.003
更新日期：2011-09-09 00:00:00
abstract：:Neuromuscular patterns associated with the development of hindlimb stepping behaviors were studied from birth to postnatal day 60 in normal kittens. Hindlimb muscles were chronically implanted with EMG electrodes at birth to characterize interlimb coordination and intralimb synergies during development of overground a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(88)90084-3
更新日期：1988-01-01 00:00:00
abstract：:A substantial number of patients do not respond sufficiently to antidepressant drugs and are therefore often co-medicated with lithium as an augmentative strategy. Since lithium has been demonstrated to affect 5-HT neurotransmission, we examined the effect of acute and subchronic lithium on 5-HT levels after a challen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02461-6
更新日期：2000-07-21 00:00:00
abstract：:This study investigated the role of strain and vendor differences on the outcome of focal cerebral ischemia in the rat induced by occlusion of the middle cerebral artery by an intraluminal thread. The cortical infarct volumes (Mean +/- S.E.M.) were: 14.2 +/- 6.2 mm3 in Simonsen Laboratories Sprague-Dawley rats; 84.0 +...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00033-m
更新日期：1995-03-27 00:00:00
abstract：:The purpose of this study was to determine whether treatment with high-dose human serum albumin (HSA) would offer protection in a model of high-grade transient forebrain ischemia. Twenty-six fasted Wistar rats underwent bilateral common carotid artery occlusion and severe hypotension (50 mmHg) for 10 min. The agent (2...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01952-6
更新日期：1999-10-16 00:00:00
abstract：:SC1 is an extracellular matrix glycoprotein that is related to the multifunctional protein SPARC. These matricellular members play regulatory roles in modulating cellular interactions. SC1 expression is enriched in the central nervous system during embryonic and postnatal development as well as in the adult brain. In ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02270-9
更新日期：2002-03-29 00:00:00
abstract：:A gentle long-lasting handling produces persistent neurochemical and behavioural changes and attenuates the impairment in the behavioural reactivity to novelty induced by the prenatal exposure to diazepam (DZ) in adult male rat progeny. This study investigated the consequences of a late prenatal treatment with three G...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03282-1
更新日期：2002-10-25 00:00:00
abstract：:The possibility that the motor cortex receives peripheral input directly from the thalamus was examined using the evoked potential method and the following results were obtained. Potentials in the motor cortex evoked by stimulation of superficial radial (SR) or group II deep radial (DR) nerve were neither abolished no...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90532-8
更新日期：1979-08-24 00:00:00
