Abstract：
:The effect of lactic acidosis on calcium influx, accumulation and efflux was studied in primary cultures of neonatal cortical rat astrocytes. Treatment of cultures with 20 mM sodium lactate, pH 6.0, for 10-60 min resulted in a 35% reduction of 45Ca2+ influx. The decrease in calcium influx was pH dependent because a similar reduction was observed in cultures exposed to pH 6.0 without lactate, while no difference was observed in cultures treated with sodium lactate at pH 7.4. Calcium accumulation was also decreased by lactic acidosis (20% reduction), while calcium efflux was unaffected. Studies with lanthanum, an inhibitor of calcium transport, indicated that the effect of lactic acidosis was not due to non-specific leakage of calcium. The reduction in calcium influx was reversible, thereby indicating that the cells were not permanently damaged by lactic acidosis. In addition to basal calcium influx, stimulated influx (mediated by extracellular ATP, 100 microM) was also reduced by 20 mM sodium lactate, pH 6. These findings suggest that protonization of calcium channels or other calcium entry pathways leads to a reduction in calcium influx in astrocytes. This diminished calcium entry, by affecting calcium-dependent mechanisms necessary for such processes as volume regulation, glycogen metabolism, or regulation of ionic permeability, may alter the ability of astrocytes to elicit appropriate responses following CNS injury.
journal_name
Brain Resjournal_title
Brain researchauthors
Neary JT,Fu Q,Bender AS,Norenberg MDdoi
10.1016/0006-8993(93)90371-ssubject
Has Abstractpub_date
1993-02-26 00:00:00pages
211-6issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(93)90371-Sjournal_volume
604pub_type
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