Abstract:
:5-Hydroxytryptamine (5-HT)-like immunoreactive neurones were studied during the development of myenteric plexus explant cultures from the guinea-pig taenia coli over a period of 3 weeks in vitro, using immunofluorescence histochemistry. Brightly fluorescent 5-HT-like immunoreactive neuronal cell bodies and fibres were found in all ages of cultures examined. In mature cultures, where the histotypic organization resembles that of the plexus in vivo, the pattern of immunoreactivity was strikingly similar to that previously described for in situ preparations. These culture preparations may therefore be useful models for the study of the development of putative serotonergic neurones. High-performance liquid chromatography was used to measure 5-HT levels in fetal calf serum, a supplement of the culture medium used in this study. 5-HT levels of 0.48 X 10(-5) M to 1.74 X 10(-4) M were found in 4 batches of this serum, indicating that some of the immunoreactive neurones observed in the cultures may have selectively taken up 5-HT during development in vitro.
journal_name
Brain Resjournal_title
Brain researchauthors
Saffrey MJ,Legay C,Burnstock Gdoi
10.1016/0006-8993(84)90865-5subject
Has Abstractpub_date
1984-06-18 00:00:00pages
105-16issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(84)90865-5journal_volume
304pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::We previously reported that the antinociceptive effect of morphine on the thermal tail withdrawal reflex (tail flick, TF) was reduced in rats that had practiced the TF response prior to morphine administration. This phenomenon, termed behavioral tolerance, was observed even when rats were spinally transected following...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90342-7
更新日期:1992-05-22 00:00:00
abstract::The phenomenon whereby the relative timing between presynaptic and postsynaptic spiking determines the direction and extent of synaptic changes in a critical temporal window is known as spike timing-dependent synaptic plasticity (STDP). We have previously reported that STDP profiles can be classified into two types de...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2013.05.023
更新日期:2013-08-14 00:00:00
abstract::To determine whether the sympathoexcitatory projection from the rostral ventrolateral medulla (RVL) to the sympathetic intermediolateral nucleus (IML) of the spinal cord might use glutamate as an excitatory transmitter, we performed a dual-label, transport and immunocytochemical ultrastructural study. Axon terminals w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91196-8
更新日期:1991-10-18 00:00:00
abstract::The putative neurotransmitter substance P was localized in the embryonic and neonatal mouse trigeminal nerve by indirect immunofluorescence. Central terminals in the nucleus caudalis gave a positive substance P-like immunofluorescence (SPLI) reaction of E13-E14. SPLI subsequently appeared in the peripheral mucous and ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(82)90147-x
更新日期:1982-07-01 00:00:00
abstract::The avian pineal exhibits a daily rhythm in the synthesis and secretion of the hormone, melatonin, which is involved in maintaining temporal order within the circadian system of some species. The pineal is richly innervated by sympathetic nerves which originate in the superior cervical ganglia (SCG) and, in the chicke...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90636-n
更新日期:1992-01-13 00:00:00
abstract::Astrocytes associated with beta-amyloid (Aβ)-deposited senile plaques are a common neuropathological feature of Alzheimer's disease (AD). There is little doubt that the association of Aβ with the major component in the central nervous system cells significantly influences disease progression, however, the molecular me...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.12.062
更新日期:2012-03-02 00:00:00
abstract::Spinal cord injury (SCI) often results in some form of paralysis. Recently, SCI therapy has been focused on preventing secondary injury to reduce both neuroinflammation and lesion size so that functional outcome after an SCI may be improved. Previous studies have shown that adenosine receptors (AR) are a major regulat...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.12.053
更新日期:2016-03-01 00:00:00
abstract::Histochemical investigation by means of light and electron microscopy revealed the presence of dipeptidylpeptidase IV activity in Meissner corpuscles of macaque glabrous skin. The enzyme activity was found in fibroblast-like cells forming an incomplete capsule around the Meissner corpuscle. Distinct electron-dense rea...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90739-1
更新日期:1988-09-27 00:00:00
abstract::This study investigated the effects of REM (rapid eye movement) sleep deprivation (RSD) on the activity of central cholinergic receptors and passive avoidance retention in rats treated neonatally with clomipramine. Male rat pups treated with clomipramine (15 mg/kg, s.c.) twice daily from postnatal day 5 to 21 were sub...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02248-4
更新日期:2000-06-09 00:00:00
abstract::Signal transducer and activator of transcription 3 (STAT3) is a transcription factor abundantly expressed in the postnatal brain that is involved in the differentiation of cultured astrocytes. Thus far, the cellular identity and anatomical distribution of STAT3-expressing cells in the postnatal brain is poorly known. ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.04.115
更新日期:2006-07-07 00:00:00
abstract::We have previously described a rodent model of brief (30 min) middle cerebral artery occlusion followed by reperfusion, in which infarction develops gradually, reaching completion more than 3 days after ischemia, accompanied by morphological, biochemical, and pharmacological evidence of apoptosis. In the present study...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02822-0
更新日期:2001-11-02 00:00:00
abstract::Large bilateral aspiration lesions of the hippocampus had no significant effect on septal kindling, whereas large bilateral DC lesions of the pyriform lobe resulted in a small but significant increase in the number of septal stimulations required to complete kindling. Bilateral aspiration lesions of the dorsal hippoca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90826-8
更新日期:1988-06-28 00:00:00
abstract::Experimental studies and clinical observations show that spinal cord lesions are greatly enlarged by a process called secondary cell death. A detailed understanding of the molecular and cellular processes underlying these events is still lacking. In clinical studies using methylprednisolone in spinal cord injured pati...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01410-j
更新日期:1995-02-20 00:00:00
abstract::The present experiments were designed to evaluate pharmacologically the role of three neuroanatomically related systems--dopamine, glutamate and GABA--in the motor-stimulant response to amphetamine and cocaine. The data indicate that stimulant-induced stereotypy is blocked by antagonists of all three systems and that ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01334-e
更新日期:1995-02-06 00:00:00
abstract::This study investigated the relationship between central cholinergic neurons and insulin secretion in bilateral adrenalectomized fed rats. Neostigmine (a cholinesterase inhibitor, 5 x 10(-8) mol) administered into the third cerebral ventricle produced significant increases in hepatic venous plasma insulin and glucose ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91003-2
更新日期:1989-07-24 00:00:00
abstract::Anandamide, the ethanolamide of arachidonic acid, is an endogenous cannabinoid. It is an agonist at CB1 and CB2 cannabinoid receptors as well as the vanilloid receptor, VR1. It is analgesic in inflammatory and neuropathic pain. Both central and peripheral mechanisms are considered to participate in its analgesia. Prim...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.09.004
更新日期:2005-11-16 00:00:00
abstract::The purpose of the present study is to characterize the striatum of the lamprey by immunohistochemical and tracing techniques. Cells immunoreactive for GABA and substance P (SP), and positive for acetylcholinesterase, are present in the lamprey striatum. Immunoreactive (ir) fibers were detected by antisera raised agai...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00701-4
更新日期:1997-08-22 00:00:00
abstract::Intracellular assessments of the physiological actions of cannabinoid receptor agonists and antagonists on adult hippocampal CA1 pyramidal cells in the in vitro slice preparation were performed using current clamp and conventional sharp-electrode intracellular recording procedures. Several manipulations were performed...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02110-7
更新日期:2000-04-28 00:00:00
abstract::In the present study we evaluated the role of NMDA receptors on the pressor and bradycardic responses to L-glutamate (L-Glu) microinjected into the nucleus tractus solitarius (NTS) of unanesthetized rats. L-Glu (1 nmol/100 nl) was microinjected into the NTS before and 10 min after microinjection of phosponovaleric aci...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/S0006-8993(96)01169-9
更新日期:1997-02-28 00:00:00
abstract::Following injection of the acylating reagent N-succinimidyl [3H]propionate into the optic nerve of goldfish, labeled protein appeared in the ipsilateral retina and contralateral tectum in a time-dependent manner. Autoradiography indicated the presence of the labeled material in the neuroplasm of the retinal ganglion c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)91251-9
更新日期:1983-01-24 00:00:00
abstract::Here we describe a sustained outward potassium current (IK) in retinal horizontal cells (HCs). IK is unusually small over the range of membrane potentials normally experienced by these cells, which are chronically depolarized. We hypothesize that this unique IK will reduce the amount of neurotransmitter required to sh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90204-o
更新日期:1990-09-24 00:00:00
abstract::Glia represents the most numerous group of nervous system cells and CNS development and function depend on glial cells. We developed a purified Muller glia culture to investigate the expression of several neurotransmitter markers on these cells, such as dopaminergic, cholinergic, GABAergic and peptidergic receptors or...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.01.031
更新日期:2005-03-21 00:00:00
abstract::Animal and cell experiments showed that inositol hexaphosphate (IP6) was protective on neurons in parkinson's disease (PD) model, but the underlying mechanism of this action was not extensively elucidated. To address this question, we established 6-hydroxydopamine (6-OHDA) induced human dopaminergic cell line SH-SY5Y ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.12.035
更新日期:2016-02-15 00:00:00
abstract::In the present study we analyzed the effect of ascorbate (0.8 mM)/Fe2+ (2.5 microM)-induced membrane lipid peroxidation on the levels of intracellular free calcium,[Ca2+]i and on the possible mechanisms involved in the perturbation of intracellular calcium homeostasis during oxidative stress. For this purpose, the inf...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01554-x
更新日期:1996-03-25 00:00:00
abstract::In the present investigation, electron probe X-ray microanalysis was used to characterize the effects of in vitro ouabain (2 mM) or anoxia on elemental composition (e.g. Na, K, Ca) and water content of rat peripheral (tibial) nerve myelinated axons and Schwann cells. Results showed that independent of axon size, both ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00855-k
更新日期:1995-10-02 00:00:00
abstract::Perfusion of artificial CSF from a lateral ventricle to the cerebral cortical subarachnoid space in anaesthetized cats was used to study the contribution of the cerebral cortex to the concentration of 5-hydroxyindoleacetic acid (5-HIAA) in CSF. The technique did not cause rupture of the meninges. The use of [14C]5-HIA...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91187-2
更新日期:1981-03-23 00:00:00
abstract::The D stereoisomer of dihydroxyphenylalanine (D-DOPA) and its alpha-keto acid metabolite 3,4-dihydroxyphenylpyruvic acid (DHPPA) when infused into the striatum, significantly increased in vivo extracellular dopamine levels. Following D-DOPA administration, the cumulative increase in dopamine levels was 30% of the incr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00116-3
更新日期:1996-04-29 00:00:00
abstract::Recent evidence suggests that cocaine addiction may involve progressive drug-induced neuroplasticity of the dorsal striatum. Here, we examined the effects of a) dorsolateral caudate putamen (dlCPu) lesions on cocaine self-administration, extinction of responding, and subsequent reinstatement to cocaine-seeking, and b)...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.08.030
更新日期:2011-10-12 00:00:00
abstract::Several studies have recently shown that basal and stress-induced secretion of corticosterone may enhance vulnerability to drugs of abuse. In this report, we studied the effects of metyrapone, an inhibitor of the synthesis of corticosterone, on cocaine-induced locomotion and on the relapse of cocaine self-administrati...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(09)90034-8
更新日期:1994-09-26 00:00:00
abstract::The cholinergic presynaptic terminals of Torpedo electric organ have been examined morphometrically following stimulation by KCI and sucrose. The objective was to confirm correlations predicted by the vesicle hypothesis between miniature end-plate potentials (MEPPs) and morphometric changes in terminal ultrastructure....
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00109-1
更新日期:1997-04-25 00:00:00