Characterization of STAT3-expressing cells in the postnatal rat brain.


:Signal transducer and activator of transcription 3 (STAT3) is a transcription factor abundantly expressed in the postnatal brain that is involved in the differentiation of cultured astrocytes. Thus far, the cellular identity and anatomical distribution of STAT3-expressing cells in the postnatal brain is poorly known. This study identifies the cell type(s), anatomical location, and temporal distribution of STAT3-expressing cells by using immunohistochemistry and confocal microscopy on postnatal day 3 (P3), 10 (P10), and 21 (P21) rat brain sections. Furthermore, the phosphorylation of STAT3 on tyrosine and serine residues was analyzed at these different stages by immunoprecipitation followed by Western blot. STAT3 immunoreactivity was observed in the cytoplasm and nucleus of many maturating astrocytes positive for nestin (at P3) or positive for GFAP (at P10) distributed throughout the white and grey matter. Moreover, robust nuclear immunoreactivity was observed in brainstem motoneurons. Phosphorylation on tyrosine and serine was observed at P3 and increased at P10, which suggests an augmented activation of STAT3 at the mid-postnatal period. At P21, STAT3 immunoreactivity dramatically decreased to remain visible only in the cytoplasm of white matter astrocytes and hypothalamic and brainstem neuronal groups. Furthermore, while the phosphorylation of tyrosine residues tended to decrease, that of serine residues further increased. In summary, our study reveals a complex regulation of STAT3 phosphorylation in the postnatal brain and provides in vivo evidence of the specific expression of STAT3 in maturating astrocytes and brainstem motoneurons.


Brain Res


Brain research


Gautron L,De Smedt-Peyrusse V,Layé S




Has Abstract


2006-07-07 00:00:00














  • Neuroblastoma cells in neural transplants: a neuroanatomical and behavioral analysis.

    abstract::The present experiments were aimed to determine the extent to which differentiated neuroblastoma cells may serve as a donor source for neural transplantation studies. Rodent-derived C1300 and human-derived LA-N-2 cells stained positively for choline acetyltransferase in vitro whether left untreated or rendered amitoti...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kordower JH,Notter MF,Gash DM

    更新日期:1987-08-04 00:00:00

  • Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair.

    abstract::A basic experiment of peripheral nerve regeneration using neuronal progenitor cells embedded in collagen gel was performed in a rat sciatic nerve defect. First, when neuronal progenitor cells derived from the fetal rat hippocampus were cultured in atelocollagen-containing medium, neurospheres positive for anti-nestin ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Murakami T,Fujimoto Y,Yasunaga Y,Ishida O,Tanaka N,Ikuta Y,Ochi M

    更新日期:2003-06-06 00:00:00

  • Distribution of [3H]AMPA binding sites in rat brain as determined by quantitative autoradiography.

    abstract::Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) is a potent neuroexcitatory compound which acts at the quisqualate class of excitatory amino acid receptors. In this study we describe the pharmacological characteristics and anatomical distribution of [3H]AMPA binding sites in rat brain using quantitativ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Monaghan DT,Yao D,Cotman CW

    更新日期:1984-12-17 00:00:00

  • Maturation but not survival of dopaminergic nigrostriatal neurons is affected in developing and aging BDNF-deficient mice.

    abstract::Brain-derived neurotrophic factor (BDNF) promotes survival of injured dopaminergic nigrostriatal neurons of the adult rodent substantia nigra pars compacta, as well their development in vitro. BDNF deficiency may play a role in Parkinson's disease, as the surviving dopaminergic nigrostriatal neurons have reduced level...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Baker SA,Stanford LE,Brown RE,Hagg T

    更新日期:2005-03-28 00:00:00

  • Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity.

    abstract::Lycium barbarum is an oriental medicinal herb that has long been used for its anti-aging and cell-protective properties. Previous studies have shown that aqueous extracts from L. barbarum exhibit neuroprotection via inhibiting pro-apoptotic signaling pathways. Other active components can also be accomplished by novel ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ho YS,Yu MS,Lai CS,So KF,Yuen WH,Chang RC

    更新日期:2007-07-16 00:00:00

  • Long-lasting conditioning of the human soleus H reflex following quadriceps tendon tap.

    abstract::Percussion of the quadriceps tendon was used to test the hypothesis that knee extensor muscle spindle discharge initiates down-regulation of the gain of the soleus H reflex. Seven subjects participated. Soleus H reflex magnitude was observed for up to 15 s, following conditioning tendon taps of 60 N or 80 N force and ...

    journal_title:Brain research

    pub_type: 临床试验,杂志文章


    authors: Cheng J,Brooke JD,Staines WR,Misiaszek JE,Hoare J

    更新日期:1995-05-29 00:00:00

  • Glucose administration after traumatic brain injury exerts some benefits and no adverse effects on behavioral and histological outcomes.

    abstract::The impact of hyperglycemia after traumatic brain injury (TBI), and even the administration of glucose-containing solutions to head injured patients, remains controversial. In the current study adult male Sprague-Dawley rats were tested on behavioral tasks and then underwent surgery to induce sham injury or unilateral...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Shijo K,Ghavim S,Harris NG,Hovda DA,Sutton RL

    更新日期:2015-07-21 00:00:00

  • Effect of central and peripheral administration of a nitric oxide synthase inhibitor on morphine hyperthermia in rats.

    abstract::The effect of central and peripheral administration of a nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on morphine hyperthermia was studied in male Sprague-Dawley rats. The first series of experiments examined the effect of subcutaneous (s.c.) administration of L-NAME on the hyperthermia i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Benamar K,Xin L,Geller EB,Adler MW

    更新日期:2001-03-16 00:00:00

  • The role of the medial prefrontal cortex of rats in short-term memory functioning: further support for involvement of cholinergic, rather than dopaminergic mechanisms.

    abstract::The putative involvement of the dopaminergic innervation of the medial part of the prefrontal cortex (PFC) in short-term memory functioning was investigated by evaluating the effects of local infusions of dopaminergic drugs into the ventral part of the medial PFC of rats in an operant delayed-matching-to-position (DMT...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Broersen LM,Heinsbroek RP,de Bruin JP,Uylings HB,Olivier B

    更新日期:1995-03-20 00:00:00

  • Magnetic resonance spectroscopic analysis of neurometabolite changes in the developing rat brain at 7T.

    abstract::We utilized proton magnetic resonance spectroscopy to evaluate the metabolic profile of the hippocampus and anterior cingulate cortex of the developing rat brain from postnatal days 14-70. Measured metabolite concentrations were modeled using linear, exponential, or logarithmic functions and the time point at which th...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ramu J,Konak T,Liachenko S

    更新日期:2016-11-15 00:00:00

  • Differential blocking effects of a spider toxin on synaptic and glutamate responses in the afferent synapse of the acoustico-lateralis receptors of Plotosus.

    abstract::The hypothesis that glutamate is the afferent transmitter in the acoustico-lateralis receptors was examined in Plotosus electroreceptors. JSTX , a spider toxin known to specifically block glutamate receptors, irreversibly abolished afferent impulse discharges induced by iontophoretically applied glutamate, whereas tho...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Nagai T,Obara S,Kawai N

    更新日期:1984-05-21 00:00:00

  • [1-14C]Octanoate: a fast functional marker of brain activity.

    abstract::[1-14C]Octanoate (OCTO) is rapidly extracted from blood and metabolized by brain. Up to 6 min following an intravenous pulse injection the autoradiographic pattern of brain labeling reflects differences in blood flow and energy metabolism. When animals are given OCTO during visual or somatosensory stimulation the auto...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Rowley H,Collins RC

    更新日期:1985-06-03 00:00:00

  • Afferents to the central nucleus of the amygdala and functional subdivisions of the periaqueductal gray: neuroanatomical substrates for affective behavior.

    abstract::Evidence suggests the periaqueductal gray (PAG) is involved in the integration of behavioral and autonomic components of affective behavior. Our laboratory has shown that electrical stimulation of the ventrolateral periaqueductal gray (vl PAG) versus the dorsolateral periaqueductal gray (dl PAG), in the rabbit, elicit...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Paredes J,Winters RW,Schneiderman N,McCabe PM

    更新日期:2000-12-22 00:00:00

  • Glycine and GABAA receptor-mediated chloride fluxes in synaptoneurosomes from different parts of the rat brain.

    abstract::Strychnine-sensitive, inhibitory glycine receptors have not until lately been considered to play a significant role in neurotransmission in mammalian forebrain regions. In order to investigate the role of glycine as a neurotransmitter in brain we have measured glycine induced chloride fluxes in different adult rat for...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Engblom AC,Eriksson KS,Akerman KE

    更新日期:1996-03-11 00:00:00

  • Organization of layers II-III connections in human visual cortex revealed by in vitro injections of biocytin.

    abstract::In the search for cortical mechanisms subserving psychological phenomena, a better understanding of human cortical circuitry is crucial. In this report we describe aspects of intrinsic connectivity of supragranular layers in human visual cortex, revealed by extracellular injections of the anterograde tracer biocytin i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kenan-Vaknin G,Ouaknine GE,Razon N,Malach R

    更新日期:1992-10-30 00:00:00

  • Lithium modulates cortical excitability in vitro.

    abstract::The sometimes devastating mood swings of bipolar disorder are prevented by treatment with selected antiepileptic drugs, or with lithium. Abnormal membrane ion channel expression and excitability in brain neurons likely underlie bipolar disorder, but explaining therapeutic effects in these terms has faced an unresolved...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Butler-Munro C,Coddington EJ,Shirley CH,Heyward PM

    更新日期:2010-09-17 00:00:00

  • Presynaptic localization of omega-conotoxin-sensitive calcium channels at the frog neuromuscular junction.

    abstract::By the use of anti-omega-CTx antibodies in indirect immunofluorescence we demonstrated the presence of omega-CTx binding sites in the presynaptic compartment of frog nerve-muscle preparations. The images we obtained indicate that omega-CTx-sensitive channels are clustered at discrete sites corresponding in distributio...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Torri Tarelli F,Passafaro M,Clementi F,Sher E

    更新日期:1991-05-03 00:00:00

  • Ionic dependence of early adaptation in the crustacean stretch receptor.

    abstract::In the present study we have examined the effects of changes in potassium and calcium concentration on the early adaptation of the slowly adapting stretch receptor of the crayfish using intracellular recordings including the potential clamp technique. This was because previous studies had suggested that the early adap...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ottoson D,Swerup C

    更新日期:1985-06-10 00:00:00

  • The pharmacology of cholinergic excitatory responses in hippocampal pyramidal cells.

    abstract::The pharmacology of excitatory cholinergic responses in CA1 pyramidal cells was examined in detail using intracellular recording from the hippocampal slice preparation. Acetylcholine (ACh), carbachol, muscarine and pilocarpine depolarized the membrane potential with an associated increase in input resistance. In addit...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Cole AE,Nicoll RA

    更新日期:1984-07-09 00:00:00

  • The morphogenesis of glutamic acid decarboxylase in the neostriatum of the cat: neuronal and ultrastructural localization.

    abstract::Correlative light and electron microscopic immunohistochemical methods were adapted for a descriptive analysis of the normal time course and pattern of expression and intraneuronal localization of the enzyme glutamic acid decarboxylase (GAD) in the neostriatum (Ns) of fetal, postnatal and adult cats. The differentiati...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fisher RS,Levine MS,Adinolfi AM,Hull CD,Buchwald NA

    更新日期:1987-06-01 00:00:00

  • Effect of auditory neocortex ablation on identification of click rates in cats.

    abstract::Six experimental and 3 unoperated cats were trained with a go, no-go shock avoidance procedure to discriminate increases from decreases in the rate of presentation of all auditory cortex between the suprasylvian sulcus and rhinal fissure while 3 cats had bilateral auditory cortex lesions plus ablation of the cortex of...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Cranford JL,Igarashi M,Stramler JH

    更新日期:1976-10-29 00:00:00

  • Neuropeptides of Drosophila related to molluscan neuropeptides: dependence of the immunoreactivity pattern on the ontogenetic stage and functional state.

    abstract::The CNP neuropeptides (Command Neuron Peptides) were first found in the command neurons for withdrawal behavior in the terrestrial snail. Given the fact that certain peptides can be found in various invertebrates, we examined Drosophila brains to determine if CNP-like peptides were present. Two types of antisera were ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ierusalimsky VN,Balaban PM

    更新日期:2007-06-04 00:00:00

  • A comparison of human tactile stimulus velocity discrimination with the ability of S-I cortical neurons in awake rhesus monkeys to signal the same velocity differences before and after non-anesthetic doses of pentobarbital.

    abstract::Using psychophysical techniques, the ability of human subjects to discriminate the velocity of brush movement across the glabrous skin of the hand was determined. The same stimulus was then used in a series of neurophysiological experiments designed to determine the ability of neurons in the primary somatosensory cort...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Collins JG,Roppolo JR

    更新日期:1980-10-06 00:00:00

  • Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia.

    abstract::Ischemic stroke is caused by the interruption of cerebral blood flow that leads to brain damage with long-term sensorimotor deficits. Stem cell transplantation may recover functional deficit by replacing damaged brain. In this study, we attempted to test whether the human neural stem cells (NSCs) can improve the outco...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Chu K,Kim M,Park KI,Jeong SW,Park HK,Jung KH,Lee ST,Kang L,Lee K,Park DK,Kim SU,Roh JK

    更新日期:2004-08-06 00:00:00

  • Effect of thyrotropin releasing hormone on U-50,488H-induced pharmacological responses in mice.

    abstract::The effect of thyrotropin releasing hormone (TRH) administered either subcutaneously (s.c.) or intracerebroventricularly (i.c.v.) on the analgesic and hypothermic actions of U-50,488H, a highly selective kappa-opiate agonist, was determined in male Swiss Webster mice. Intraperitoneal administration of U-50,488H (8-32 ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Bhargava HN,Thorat SN

    更新日期:1993-10-15 00:00:00

  • Ultrastructural analysis of medial brain stem afferents to the superficial dorsal horn.

    abstract::Axonal projections of medial brain stem areas rich in serotonin-containing neurons were identified in layers I and II of cat medullary dorsal horn using EM autoradiography. Following [3H]amino acid injections into the brain stem, labeled axonal endings were found throughout layers I and II but were most numerous in la...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Ruda MA,Allen B,Gobel S

    更新日期:1981-01-26 00:00:00

  • Effects of early monocular deprivation on choline acetyltransferase and glutamic acid decarboxylase in pigeon visual Wulst.

    abstract::Choline acetyltransferase (ChAT) and glutamic acid decarboxylase (GAD) activities were measured in various visual structures of the pigeon brain after long-term monocular deprivation followed by short-term binocular presence or absence of light stimulation. The short-term phase (45 min) was coupled with a 2-deoxygluco...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Bagnoli P,Burkhalter A,Vischer A,Henke H,Cuénod M

    更新日期:1982-09-16 00:00:00

  • Modulation of the myotatic reflex gain in man during intentional movements.

    abstract::Human subjects were asked to perform sinusoidal tracking movements (0.5--3.0 Hz) with their forearms while external torque disturbances were applied at the elbow. The changes in angular position, velocity, and acceleration produced by these disturbances were found to be represented in the reflex changes in EMG activit...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Dufresne JR,Soechting JF,Terzuolo CA

    更新日期:1980-07-07 00:00:00

  • Immunohistochemical localization of calpains and calpastatin in the rabbit eye.

    abstract::The localization of the two Ca-activated extralysosomal proteases m-calpain and mu-calpain in the eye of the adult rabbit was examined by immunohistochemistry, using poly- and monoclonal antibodies against the corresponding rabbit antigens. Immunoreactivity against the two forms of calpains was observed in the epithel...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Persson H,Kawashima S,Karlsson JO

    更新日期:1993-05-21 00:00:00

  • Release of cholecystokinin from rat midbrain slices and modulatory effect of D2DA receptor stimulation.

    abstract::Cholecystokinin octapeptide (CCK-8) is colocalized within a majority of dopamine (DA)-containing neurons of the rat midbrain. Exogenous CCK-8 can modulate the electrophysiological activity of DA neurons, at least in part, by direct actions on the somatodendritic region of these cells. If CCK-8 is somatodendritically r...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Freeman AS,Chiodo LA,Lentz SI,Wade K,Bannon MJ

    更新日期:1991-08-02 00:00:00