Abstract:
:The initiating mechanisms involved in colonic injury are currently unknown. The goal of the current study was to examine the role of the inflammatory mediators reactive oxygen metabolites and proteases in an ex vivo model of selective epithelial permeability. Rats were prepared with exteriorized colonic chambers to which the protein kinase C (PKC) activator phorbol myristate acetate (PMA) was added in doses ranging from 5 to 800 micrograms. PMA caused a dose-dependent transient increase in epithelial permeability, but had no significant effect on microvascular permeability. There was no accumulation of neutrophils and no apparent histological changes. PMA acts via a PKC-dependent mechanism, as assessed using the PKC-inactive phorbol analog 4 alpha-phorbol didecanoate, and the response is tachyphylactic. The mechanism is independent of reactive oxygen metabolites and proteases, as shown by the lack of effect of the free radical scavengers superoxide dismutase and catalase and the general serine protease inhibitor soybean trypsin inhibitor. The classic inflammatory process does not appear to be involved in the PMA-induced epithelial permeability changes. This finding suggests that noninflammatory mechanisms may regulate the increased epithelial permeability induced by PMA. Further study to elucidate these mechanisms is of importance for understanding both normal gastrointestinal physiology and initiation of pathology.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Berin MC,Buell MGdoi
10.1007/BF02209017subject
Has Abstractpub_date
1995-10-01 00:00:00pages
2268-79issue
10eissn
0163-2116issn
1573-2568journal_volume
40pub_type
杂志文章abstract:BACKGROUND:Oxaliplatin is one of the most effective chemotherapeutic drugs used for the treatment of colorectal cancer (CRC). However, intervention that attenuates the resistance of oxaliplatin is still required in the treatment of CRC. AIMS:To investigate the role of miR-325 in changing the oxaliplatin sensitivity to...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-020-06579-7
更新日期:2020-09-10 00:00:00
abstract::After gastrectomy, Roux-Y limb reconstruction leads to duodenojejunal motor disturbances. Because motilin and pancreatic polypeptide (PP) play a role in the regulation of digestive motility, their plasma concentrations were determined in rats after Roux-Y gastrectomy. Three months after a distal Roux-Y gastrectomy, co...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1005548524334
更新日期:2000-08-01 00:00:00
abstract::Despite progress in therapeutic approaches for the elimination of hepatitis C, chronic hepatitis C virus infection remains an important cause of liver disease. Therapeutic intervention with the currently available interferon-based treatment regimens is quite successful, but treatment is difficult to tolerate and is co...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,评审
doi:10.1007/s10620-006-9484-7
更新日期:2007-04-01 00:00:00
abstract::We have studied pepsinogen synthesis and secretion in primary monolayer cultures of canine gastric chief cells. Monolayers were formed after approximately 48 hr. Pepsinogen synthesis was studied by adding 14C-labeled amino acids to the culture medium. Basal secretion of de novo synthesized pepsinogen after 4 hr was 4 ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01535950
更新日期:1988-12-01 00:00:00
abstract::Two new preparations are under clinical trial for use in the treatment of ulcerative proctitis and left-sided colitis. One is 5-aminosalicylate, a component of sulfasalazine. This substance has virtually no immunomodulating capabilities. Evidence for efficacy equal to that of sulfasalazine in acute colitis has been ob...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章
doi:10.1007/BF01538136
更新日期:1988-03-01 00:00:00
abstract::The aims of our randomized, single-blind trial involving 49 patients were to study the efficacy, side effects, and tolerance of metronidazole and satranidazole in patients of amebic liver abscess. Twenty-five patients received metronidazole (800 mg TID) and 24 received satranidazole (300 mg TID with placebo at mealtim...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s10620-006-9111-7
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:Numerous experiments have shown that alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in cells of various cancer and can play role in carcinogenesis. In previous investigations we have found elevated levels of alcohol dehydrogenase class III activity in pancreatic cancer cells. It ca...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-007-0144-3
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:Fulminant hepatic failure (FHF) is a devastating syndrome, which sometimes results in death or liver transplantation, in which inflammation would aggravate the development of fetuin-A which would act as an anti-inflammatory factor and may be an available approach to attenuate FHF. AIMS:The purpose of this s...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-014-3071-0
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Accumulating evidence has indicated that variations of mitochondrial DNA (mtDNA) content may affect the susceptibility to hepatocellular carcinoma (HCC). However, no study has been conducted to evaluate the association of circulating mtDNA content and the risk of liver cirrhosis, a leading cause of ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-015-3523-1
更新日期:2015-06-01 00:00:00
abstract::We have compared the [14C]triolein breath test for fat malabsorption with fecal fat excretion corrected for marker pellet recovery in 23 subjects with chronic liver disease. The breath test identified 15 of the 17 subjects with abnormal fecal fat excretion (sensitivity 88%). However, four of the six subjects with norm...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01798358
更新日期:1988-05-01 00:00:00
abstract::Cyclic vomiting syndrome is a disorder characterized by recurrent episodes of nausea and vomiting with complete resolution of symptoms between attacks. Nitric oxide plays a critical role in regulating several components of gastrointestinal mucosal defense and injury. Interleukin-6 has a wide variety of actions in the ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1010712703685
更新日期:2001-04-01 00:00:00
abstract::Helicobacter pylori infection has been linked with gastric carcinoma. Epithelial cell proliferation is an indicator of cancer risk. The aim of this study was to assess gastric epithelial cell proliferation before and after eradication therapy and to assess the efficacy of treatment of H. pylori infection using lanzopr...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章
doi:10.1007/BF02212681
更新日期:1995-08-01 00:00:00
abstract::In a retrospective study, 50 patients with obvious necrotizing pancreatitis (NP) were allocated in four groups according to the morphological aspects of the necrosis. Appearance of ascite (N1), extrapancreatic spread of necrosis towards neighboring organs (N2), a large amount of necrosis (N3), and infected necrosis (N...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1018890221620
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND:Histological remission represents a target distinct from endoscopic healing in ulcerative colitis (UC) and seems a better predictor of clinical outcomes. AIMS:The aim of this study was to assess the ability of adalimumab to achieve histological remission in UC patients. METHODS:Single-center, retrospective...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-018-4935-5
更新日期:2018-03-01 00:00:00
abstract::Clinical and experimental evidence indicates that estrogens are involved in the control of hepatocyte proliferation both in normal and in neoplastic conditions. Thirty-two cirrhotic patients with unresectable or otherwise untreatable hepatocellular carcinoma were allocated to receive either tamoxifen (30 mg/day) or no...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01296419
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:IL-1β is a cytokine involved in mediating epithelial barrier dysfunction in the gut. It is known that IL-1β mediates activation of non-muscle myosin light chain kinase in epithelial cells, but the precise mechanism by which epithelial barrier dysfunction is induced by IL-1β is not understood. METHODS AND RE...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-016-4145-y
更新日期:2016-08-01 00:00:00
abstract::On the basis of previous studies in our laboratory we postulated that the ethanol-induced alteration in jejunal morphology was the result of its effect on the microcirculation. The present study was undertaken to examine the validity of this hypothesis. Accordingly, the effects of intraluminal ethanol perfusion (3.0 a...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01311730
更新日期:1983-11-01 00:00:00
abstract::We describe the first reported case of generalized tonic-clonic seizures induced by meperidine premedication for a colonoscopy procedure in a 63-year-old woman with Alzheimer's disease. The active metabolite of meperidine, normeperidine, is postulated to be the precipitating cause of the seizures, although a cholinest...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-007-9822-4
更新日期:2008-01-01 00:00:00
abstract::The purpose of this study is to determine the long-term relapse rate and associated risk factors in HBeAg-positive chronic hepatitis B (CHB) patients who had maintained virologic response (VR) for 1 year after lamivudine (LMV) discontinuation. We enrolled 55 treatment-naive HBeAg-positive CHB patients who achieved and...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-008-0508-3
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:S-isomer (S) pantoprazole is known to be more effective and less dependent on cytochrome 2C19 than R-isomer (R)-pantoprazole. AIM:The purpose of this study was to compare the efficacy and safety of S-pantoprazole 20 mg versus pantoprazole 40 mg for treatment of reflux esophagitis. METHODS:This multi-center...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s10620-012-2297-y
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:The age to stop screening or surveillance colonoscopy is not well established, and unplanned hospital use after colonoscopy in the elderly is not well understood. AIMS:To evaluate unplanned emergency department (ED) visits and hospitalization in patients over 75 within 7 days of outpatient colonoscopy. MET...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-019-05962-3
更新日期:2020-07-01 00:00:00
abstract::Using conventional statistical analysis and multiple regression analysis, we investigated the viral and host factors that influence the response to recombinant interferon-alpha 2a therapy in patients with chronic hepatitis C. A total of 36 patients was randomly assigned to three administration schedules, 12 patients i...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02093793
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:We conducted a systematic review and meta-analysis of meat intake and esophageal cancer risk, with subgroup analyses based on meat type and histological type of cancer. AIMS:The purpose of this study was to investigate the association between meat intake and risk of esophageal cancer. METHODS:We searched M...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s10620-013-2928-y
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Although endoscopic submucosal dissection (ESD)-induced ulcers heal faster and recur less often than non-iatrogenic gastric ulcers, the optimal dosage and duration of proton pump inhibitor treatment for ESD-induced ulcers remain unclear. AIMS:To evaluate the efficacy of half-dose rabeprazole on endoscopic s...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10620-012-2436-5
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Restoring normal fecal flora through intestinal microbiota transplantation (IMT) was successful in curing recurrent Clostridium difficile infection (CDI). However, only a few cases have been reported of IMT being utilized for the treatment of severe or fulminant CDI. AIM:Is IMT a simple and effective treatm...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-014-3296-y
更新日期:2015-01-01 00:00:00
abstract::Cholecystokinin (CCK) decreases lower esophageal sphincter pressure (LESP) in man. Cholestyramine, a nonabsorbable bile salt binding resin, stimulates endogenous CCK secretion. We have investigated the effect of oral ingestion of 16 g cholestyramine without and with infusion of the CCK receptor antagonist CR1505 (loxi...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296114
更新日期:1993-10-01 00:00:00
abstract:BACKGROUND:Intestinal failure is the most critical complication of Crohn's disease. Intestinal failure requires home parenteral nutrition, which worsens the quality of life of the patients and sometimes causes life-threatening complications. AIMS:The purpose of this study was to investigate the incidence and risk fact...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-019-05553-2
更新日期:2019-08-01 00:00:00
abstract::We assessed the onset of malignant and nonmalignant complications in a cohort of celiac disease (CD) patients under gluten-free diet (GFD). Five hundred and forty-nine CD patients were retrospectively assessed. Two hundred and fifty-one (45.7%) showed classical, 262 (47.7%) subclinical, and 36 (6.6%) silent form of CD...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-008-0595-1
更新日期:2009-10-01 00:00:00
abstract::In conscious dogs with gastric and duodenal Thomas fistulas, we studied the effect of ethanol on plasma concentrations of gastrin. Ethanol was given either as an infusion into a peripheral vein (1.95 M, 200 ml/hr) or into the duodenum (0.95 M, 400 ml/hr) or as an intragastric bolus injection. The effect of the intraga...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296856
更新日期:1984-01-01 00:00:00
abstract:UNLABELLED:There is a wide choice of fecal occult blood tests (FOBTs) for colorectal cancer screening. GOAL:To highlight the issues applicable when choosing a FOBT, in particular which FOBT is best suited to the range of screening scenarios. Four scenarios characterize the constraints and expectations of screening pro...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,评审
doi:10.1007/s10620-014-3445-3
更新日期:2015-03-01 00:00:00