Abstract：
:A sustained increase in cytosolic Ca2+ can damage gastric mucosal cells. The present study has examined the role of Ca2+ in thromboxane B2 (TXB2)-mediated damage of rabbit isolated gastric mucosal cells. Cells were isolated from rabbit oxyntic mucosa by collagenase-EDTA digestion. Cell metabolic activity and cell damage were estimated by alamar blue dye absorbance and trypan blue uptake, respectively. Cellular Ca2+ was monitored by indo-1 dye fluorescence. Addition of TXB2 (10(-6) and 10(-8) M) to the cell suspension resulted in a decrease in metabolic activity, and this effect was reduced when Ca2+ was removed from the incubation medium. TXB2 addition to the incubation medium resulted in an increase in cytosolic Ca2+ and incubation of cells with the intracellular Ca2+ chelator, BAPTA-AM (20 microM), reduced cell injury in response to TXB2. Incubation of cells with the Ca2+ ionophore A23187 (1-25 microM) resulted in a dose-dependent increase in trypan blue uptake and a reduction in cell metabolism. Cell injury in response to A23187 were exacerbated by addition of TXB2 (10(-8) M) to the cell suspension. TXB2 treatment reduced cellular content of reduced glutathione (GSH), while exogenous GSH addition (10 mM) reduced TXB2-mediated cell injury. These data demonstrate that TXB2 can directly injure gastric mucosal cells. Gastric mucosal cellular damage in response to TXB2 is mediated in part by a disruption of Ca2+ homeostasis as well as a reduction in cellular GSH content.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Wong HM,Soper BD,Tepperman BLdoi
10.1007/BF02208674subject
Has Abstractpub_date
1995-09-01 00:00:00pages
2022-8issue
9eissn
0163-2116issn
1573-2568journal_volume
40pub_type
杂志文章abstract：:This study was undertaken to investigate the effect of ingestion of 80 g Vivonex on gallbladder volume, plasma cholecystokinin (CCK), and pancreatic polypeptide (PP) in eight healthy volunteers and to compare the results with those obtained after ingestion of 60 ml corn oil. Gallbladder volumes were measured by ultras...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01296686
更新日期：1987-01-01 00:00:00
abstract：:We describe the first reported case of generalized tonic-clonic seizures induced by meperidine premedication for a colonoscopy procedure in a 63-year-old woman with Alzheimer's disease. The active metabolite of meperidine, normeperidine, is postulated to be the precipitating cause of the seizures, although a cholinest...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-007-9822-4
更新日期：2008-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) arises from a number of cirrhosis-related and non-cirrhosis-related exposures and is one of the leading causes of cancer-related deaths worldwide. Achieving a durable cure currently relies on either resection or transplantation, but since most patients will be diagnosed with inoperable d...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-019-05582-x
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:The pentapeptide ghrelin agonist, relamorelin, accelerates colonic transit in patients with chronic constipation (CC). In a murine model, relamorelin decreased excitability of colonic circular smooth muscle cells and colonic intraluminal pressure. AIM:To determine short-term effects of relamorelin on coloni...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10620-015-3876-5
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND AND AIM:Atelectasis is one of the pulmonary complications associated with anesthesia. Little is known about atelectasis following endoscopic procedures under deep sedation. This study evaluated the frequency, risk factors, and clinical course of atelectasis after endoscopic resection. METHODS:A total of 349...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-015-3844-0
更新日期：2016-01-01 00:00:00
abstract：AIM:The aim of this case-control study was to investigate both the distribution of MCP-1, eNOS, tPA and PAI-1 gene polymorphism and correlation of genotypes and phenotypes. METHOD:Between September 1997-January 2005, 20 patients with HPS (group 1) were compared with a group of cirrhotic patients (group 2, n = 19) as w...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-007-0002-3
更新日期：2008-05-01 00:00:00
abstract：:In some patients, proton pump inhibitors do not abolish nocturnal gastric acidity and additional evening antisecretory medication may be required. In 16 subjects with chronic heartburn, 24-hr gastric and esophageal pH were measured at baseline and again after six days of 20 mg omeprazole alone at 08:00 hr followed by ...
journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章
doi：10.1023/a:1013749501241
更新日期：2002-02-01 00:00:00
abstract：INTRODUCTION:Crohn's disease (CD) follows a relapsing and remitting course incurring cumulative bowel damage over time. The question of whether or not the timing of the initiating biologic therapy affects long-term disease progression remains unanswered. Herein, we calculated rates of change in the Lémann index-which q...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-018-5434-4
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:Eradication therapies for Helicobacter pylori infection are advancing as new acid inhibitory reagents approved. The aim of this study was to assess the efficacy and safety of vonoprazan-based triple treatment. MATERIALS AND METHODS:Triple therapy with vonoprazan and two antibiotics (amoxicillin and clarithr...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,多中心研究,评审
doi：10.1007/s10620-017-4664-1
更新日期：2017-11-01 00:00:00
abstract：:Inhibition of monoamine oxidase B (MAO B) by selective inhibitors pargyline and L-deprenyl increases dopamine (DA) and norepinephrine (NE) concentrations in nucleus accumbens (NACB) and is associated with reduction in cold water restraint-induced gastric mucosal injury, inhibition of basal gastric acid output, and reg...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01537224
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:Percutaneous endoscopic gastrostomy (PEG) is a commonly performed procedure for patients with severe dysphagia leading to malnutrition. Improved knowledge of risk factors for PEG-related complications might decrease patient discomfort and healthcare costs. AIM:The aim of the present study was to investigate...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-013-2891-7
更新日期：2014-01-01 00:00:00
abstract：INTRODUCTION:Assessment of whole gut transit, by radio-opaque markers or scintigraphy, is used to evaluate patients with constipation for slow gastrointestinal transit. Wireless capsule motility, using the SmartPill GI monitoring system, samples and transmits intraluminal pH, pressure, and temperature data from a capsu...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-009-0899-9
更新日期：2009-10-01 00:00:00
abstract：:The effect of inhibition of acid secretion on parietal cell morphology and the concentration of H,K-ATPase alpha-subunit protein was determined by electron microscopy and western blotting. Omeprazole or famotidine alone or in combination were used. Control animals showed a morphological stimulation index (0 = resting,...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02090359
更新日期：1994-10-01 00:00:00
abstract：:The effects of ecabet sodium (ecabet), 12-sulfodehydroabietic acid monosodium salt, on gastric mucin biosynthesis in rat antrum were compared with those in the corpus. Intragastric administration of ecabet significantly increased [3H]glucosamine incorporation into antral mucin as well as into corpus mucin during five ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1005469913079
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Irritable bowel syndrome (IBS) pathophysiology has not been fully understood. Abnormalities of serine proteases have been identified in IBS patients. In addition, protease-activated receptors (PAR) activation interferes with several components of the pathogenesis of IBS, so, evaluating the PAR expression in ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-011-1827-3
更新日期：2012-01-01 00:00:00
abstract：:This paper reviews recent clinical evidence that suggests that aspirin prophylaxis against cardiac and cerebral vascular ischemia is associated with significant gastrointestinal complications. The clinical and experimental evidence to confirm the role of risk factors of concomitant use of nonsteroidal anti-inflammator...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1007/s10620-007-0178-6
更新日期：2008-10-01 00:00:00
abstract：BACKGROUND:The risk of pancreatic cancer is elevated among people with new-onset diabetes (NOD). Based on Rochester Epidemiology Project Data, the Enriching New-Onset Diabetes for Pancreatic Cancer (END-PAC) model was developed and validated. AIMS:We validated the END-PAC model in a cohort of patients with NOD using r...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-020-06139-z
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND AND AIM:The liver has a high capacity of its regeneration. Most hepatic cells are quiescent unless otherwise stimulated such as their injury or ablation. A previous study suggest that pre-activated hepatic cells have a positive effect on their regeneration. In this study, we examined whether the pre-activate...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-015-3536-9
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:Data on real-life patterns of biologic use for inflammatory bowel disease (IBD) are scarce. AIMS:We aimed to examine the patterns of biologic use and the factors associated with non-persistence and switching of biologics in Korean IBD patients. METHODS:Using National Health Insurance claims, we collected d...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-019-05867-1
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:CRP is a marker of inflammation and infection of the gastric mucosa with Helicobacter pylori, which causes an inflammatory reaction. It has been reported that CagA(+) H. pylori strains induce more severe gastric inflammation and are also associated with higher risks of peptic ulcer and gastric cancer. The pur...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-008-0686-z
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND:Major modification of the 7th tumor-node-metastasis (TNM) staging system for hepatocellular carcinoma (HCC) was divided into 6th stage IIIA to 7th IIIA (multiple tumors, any>5 cm) and IIIB (tumors involving a major vessel). This study aimed to validate 6th and 7th TNM systems in prognostic prediction, then a...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-013-2716-8
更新日期：2013-09-01 00:00:00
abstract：:Alterations in smooth muscle responsiveness and neural pathways in adjacent tissue may occur after local myenteric denervation. The in vitro contractile responses of both longitudinal and circular muscle to the mixed muscarinic and nicotinic cholinergic agonist carbachol were determined 15, 30, and 45 days after local...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01537588
更新日期：1990-09-01 00:00:00
abstract：:Stevens-Johnson Syndrome (SJS) is a rare but severe dermatological condition that typically occurs after the ingestion of medications such as nonsteroidal drugs, antibiotics, and anticonvulsants. Extracutaneous manifestations of the syndrome can occur and may involve the conjunctiva, trachea, buccal mucosa, gastrointe...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章,评审
doi：10.1023/a:1012351231143
更新日期：2001-11-01 00:00:00
abstract：:Azithromycin (Zithromax), an erythromycin derivative that belongs to a subgroup of the macrolides known as azolides, has generally been considered to be a very safe medication. Hepatic side effects are uncommon but may include jaundice, fever, and right upper quadrant pain. Herein we describe a patient who developed a...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1020170807742
更新日期：2002-10-01 00:00:00
abstract：:The peroxisome proliferator-activated receptor gamma (PPARgamma) is a nuclear receptor highly expressed in the colon and playing an anti-inflammatory role through inhibition of the NF-kappaB pathway. Toll-like receptor 4 (TLR4) has been known to mediate LPS-induced cellular signaling through activation of NF-kappaB pa...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-006-3193-0
更新日期：2006-04-01 00:00:00
abstract：:Fructose and lactose malabsorption are characterized by impaired duodenal fructose transport or by the deficiency of mucosal lactase, respectively. As a consequence, the nonabsorbed saccharides reach the colon, where they are broken down by bacteria to short fatty acids, CO2, and H2. Bloating, cramps, osmotic diarrhea...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1023/a:1005527230346
更新日期：2000-07-01 00:00:00
abstract：:The evaluation of agents potentially accelerating gastric emptying in gastric stasis syndromes is time-consuming. Since a previous study showed that emptying is slowed after antecedent fat ingestion and intravenous cisapride abolishes this effect, we investigated whether emptying delayed by fat incorporated into a mea...
journal_title：Digestive diseases and sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01307519
更新日期：1991-09-01 00:00:00
abstract：:Previous studies suggest that posture has relatively little effect on gastric emptying of high-nutrient liquids; these studies have, however, only assessed overall rates of gastric emptying, whereas gastric emptying is known to be predominantly a pulsatile phenomenon. In healthy subjects perceptions of appetite, such ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/s10620-005-9010-3
更新日期：2006-08-01 00:00:00
abstract：:Nitric oxide appears to play an important role in maintaining gastric mucosal integrity. This study aimed to investigate whether a nitric oxide donor (sodium nitroprusside) or stimulation of endogenous nitric oxide synthesis (with acetylcholine) protects against gastric ischemia-reperfusion injury. Rats were subjected...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF02090210
更新日期：1994-02-01 00:00:00
abstract：:We describe the first reported case of mantle zone lymphoma of the colon presenting as diffuse ileocolitis simulating severe inflammatory bowel disease. This case serves to illustrate the importance of immunohistochemistry in establishing the diagnosis of lymphoma in extranodal sites. ...
journal_title：Digestive diseases and sciences
pub_type： 杂志文章
doi：10.1007/BF01300394
更新日期：1992-06-01 00:00:00
