Abstract:
:Fluorescent live imaging of cells and embryos at subcellular resolution poses significant challenges for biologists due to morbidity and mortality ensuing from phototoxicity. Here we report the use of a spinning-disk confocal microscope to image mouse and bovine preimplantation embryos without impairing their developmental potential. We also present data indicating that this imaging technique does not affect the functionality of subcellular components as assessed by reactive oxygen species (ROS) production, caspase activity, and DNA integrity. Spinning-disk confocal microscopy was also useful in determining cell number and allocation in transgenic bovine blastocysts. We conclude that this imaging method is suitable for monitoring preimplantation embryos.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ross PJ,Perez GI,Ko T,Yoo MS,Cibelli JBdoi
10.2144/000112310subject
Has Abstractpub_date
2006-12-01 00:00:00pages
741-50issue
6eissn
0736-6205issn
1940-9818pii
000112310journal_volume
41pub_type
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