听力与言语-语言病理学

行为科学

医学伦理学

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  • Antibody-mediated soluble CD14 stabilization prevents agitation-induced increases in presepsin levels in blood component specimens.

    abstract::Presepsin is a 13-kDa N-terminal glycoprotein of CD14. Previously, agitation-induced increases in presepsin levels have been reported; however, the mechanism remains poorly understood. In this study, we aimed to reveal the mechanism of presepsin increase. The agitated plasma or serum was separated using gel exclusion ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0136

    authors: Morino G,Takahashi G,Kan S,Inoue Y,Sato K,Shirakawa K

    更新日期:2021-01-29 00:00:00

  • New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria.

    abstract::New cloning vectors have been developed with features to enhance quick allelic exchange in gram-negative bacteria. The conditionally replicative R6K and transfer origins facilitate conjugation and chromosomal integration into a variety of bacterial species, whereas the sacB gene provides counterselection for allelic e...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0135

    authors: Mejia-Santana A,Lloyd CJ,Klose KE

    更新日期:2021-01-25 00:00:00

  • Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products.

    abstract::Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with 'difficult-to-express' proteins. Cell-free pr...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0155

    authors: Chiba CH,Knirsch MC,Azzoni AR,Moreira AR,Stephano MA

    更新日期:2021-01-20 00:00:00

  • CRISPR vs COVID-19: how can gene editing help beat a virus?

    abstract::[Formula: see text] Known to be a sturdy weapon in a scientist's arsenal, how has the gene editing tool CRISPR been applied in the fight against COVID-19? ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/btn-2020-0145

    authors: Straiton J

    更新日期:2020-11-01 00:00:00

  • Double venipuncture is not required for adequate S-100B determination in melanoma patients.

    abstract::S-100B is used in melanoma follow-up. This serum biomarker is also present in adipocytes; therefore, subcutaneous adipocytes trapped in the needle before performing a venipuncture could contaminate the serum. The aim was to study the influence of adipocyte contamination on blood samples used for S-100B analysis, possi...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0147

    authors: Damude S,Muller Kobold AC,Bastiaannet E,Kruijff S,Hoekstra HJ,Wevers KP

    更新日期:2020-11-01 00:00:00

  • Reduced serum methods for contact-based coculture of human dermal fibroblasts and epidermal keratinocytes.

    abstract::Direct contact-based coculture of human dermal fibroblasts and epidermal keratinocytes has been a long-standing and challenging issue owing to different serum and growth factor requirements of the two cell types. Existing protocols employ high serum concentrations (up to 10% fetal bovine serum), complex feeder systems...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0112

    authors: Kadam S,Vandana M,Kaushik KS

    更新日期:2020-11-01 00:00:00

  • A device for stereotaxic viral delivery into the brains of neonatal mice.

    abstract::The increasing interest in manipulating neural circuits in developing brains has created a demand for reliable and accurate methods for delivering viruses to newborn mice. Here we describe a novel 3D-printed mouse neonatal stereotaxic adaptor for intracerebral viral injection that provides enhanced precision and relia...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0050

    authors: Olivetti PR,Lacefield CO,Kellendonk C

    更新日期:2020-10-01 00:00:00

  • Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography.

    abstract::Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity module and the new...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0080

    authors: Dai Z,Juneja J,Schneeweis L,Cohen D,Marsilio F,Morin P,DasGupta R

    更新日期:2020-09-01 00:00:00

  • A novel method for removing polyethyleneimine from biopharmaceutical samples: improving assay sensitivity of residual DNA qPCR.

    abstract::Polyethyleneimine (PEI) is a flocculent that is widely used in the downstream purification of monoclonal antibodies. It is an in-process residual that is carried through the drug purification process and strongly inhibits residual DNA quantitation by real-time quantitative PCR assay. Very high sample dilutions (e.g., ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2020-0011

    authors: Zhang SM,Roberts M,Jones M,Zeitler J,Kilby G,Liu A,White JR

    更新日期:2020-06-01 00:00:00

  • Optimization of western blotting for the detection of proteins of different molecular weight.

    abstract::Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended to medium- and low-...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0124

    authors: Heda GD,Shrestha L,Thapa S,Ghimire S,Raut D

    更新日期:2020-06-01 00:00:00

  • Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene.

    abstract::Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations...

    journal_title:BioTechniques

    pub_type: 信件

    doi:10.2144/btn-2020-0012

    authors: Gil-Gálvez A,Carbonell-Corvillo P,Paradas C,Miranda-Vizuete A

    更新日期:2020-06-01 00:00:00

  • AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays.

    abstract::Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0148

    authors: Kumar JK,Holmgren S,Levedahl KH,Höglund M,Venge P,Eriksson S

    更新日期:2020-06-01 00:00:00

  • Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension.

    abstract::We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates. The technique is ideally suited for the generation of longer donor DNA sequences for CRISPR/Cas9-medi...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0104

    authors: Hejlesen R,Füchtbauer EM

    更新日期:2020-06-01 00:00:00

  • A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue.

    abstract::Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0172

    authors: Wei S,Levy B,Hoffman N,Cujar C,Rodney-Sandy R,Wapner R,D'Alton M,Williams Z

    更新日期:2020-05-01 00:00:00

  • Evaluation of DNA degradation and establishment of a degradation analysis model for Lepidoptera specimens.

    abstract::Millions of museum specimens are integral to biodiversity studies; however, DNA degradation may limit the ability to obtain DNA sequences. In this study, a degradation analysis model for Lepidoptera specimens was established. Based on this model, we revealed the characteristics of DNA fragment distribution caused by e...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0166

    authors: Xu Y,Ren X,Wang H,Wang M,Li G

    更新日期:2020-03-01 00:00:00

  • An improved Xer-cise technology for the generation of multiple unmarked mutants in Mycobacteria.

    abstract::Xer-cise is a technique using antibiotic resistance cassettes flanked by dif sites allowing spontaneous and accurate excision from bacterial chromosomes with a high frequency through the action of the cellular recombinase XerCD. Here, we report a significant improvement of Xer-cise in Mycobacteria. Zeocin resistance c...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0119

    authors: Boudehen YM,Wallat M,Rousseau P,Neyrolles O,Gutierrez C

    更新日期:2020-02-01 00:00:00

  • Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing.

    abstract::The CRISPR/Cas9 system is an efficient gene-editing method, but it is difficult to obtain mutants for some specific species and special genome structures. A previously reported multiplexed, semi-nested PCR target-enrichment approach, which does not rely on transgenic technology, has been shown to be an effective and a...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0001

    authors: Zhang Y,Chi X,Feng L,Wu X,Qi X

    更新日期:2019-12-01 00:00:00

  • Focused size selection of cell-free DNA samples for liquid biopsy applications that rely on next-generation sequencing.

    abstract::Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0071

    authors: Raymond CK

    更新日期:2019-10-01 00:00:00

  • Efficient and robust preparation of tyrosine phosphorylated intrinsically disordered proteins.

    abstract::Intrinsically disordered proteins (IDPs) are subject to post-translational modifications. This allows the same polypeptide to be involved in different interaction networks with different consequences, ranging from regulatory signalling networks to the formation of membrane-less organelles. We report a robust method fo...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2019-0033

    authors: Brázda P,Šedo O,Kubíček K,Štefl R

    更新日期:2019-07-01 00:00:00

  • Optimizing leading edge F-actin labeling using multiple actin probes, fixation methods and imaging modalities.

    abstract::We systematically evaluated the performance and reliability of several widely used, commercially available actin-filament probes in a highly motile breast adenocarcinoma cell line to optimize the visualization of F-actin-rich dynamic lamellipodia. We evaluated four Phalloidin-fluorophores, two anti-actin antibodies, a...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0112

    authors: DesMarais V,Eddy RJ,Sharma VP,Stone O,Condeelis JS

    更新日期:2019-03-01 00:00:00

  • A method for visualizing fluorescence of flavonoid therapeutics in vivo in the model eukaryote Dictyostelium discoideum.

    abstract::Naturstoff reagent A (diphenylboric acid 2-aminoethyl ester [DPBA]) has been used historically in plant science to observe polyphenolic pigments, such as flavonoids, whose fluorescence requires enhancement to be visible by microscopy. Flavonoids are common dietary constituents and are the focus of considerable attenti...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0084

    authors: Ferrara BT,Thompson EP

    更新日期:2019-02-01 00:00:00

  • How to transition from 'early career' to 'established'.

    abstract::PhDs, postdocs, fellows and newly appointed lecturers all fall into the category of early-career researchers (ECRs). The life of researchers in early stages of their career is far from straightforward. Funding is a huge barrier for many ECRs looking to make the next step in their career, but it's not the only challeng...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/btn-2018-0182

    authors:

    更新日期:2019-01-01 00:00:00

  • Samasy: an automated system for sample selection and robotic transfer.

    abstract::Sample automation and management is increasingly important as the number and size of population-scale and high-throughput projects grow. This is particularly the case in large-scale population studies where sample size is far outpacing the commonly used 96-well plate format. To facilitate management and transfer of sa...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0090

    authors: Cario CL,Witte JS

    更新日期:2018-12-01 00:00:00

  • Software to improve transfer and reproducibility of cell culture methods.

    abstract::Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax® as a tool for st...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0062

    authors: Canfield SG,Jin G,Palecek SP,Sampsell T

    更新日期:2018-11-01 00:00:00

  • A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria.

    abstract::A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this wor...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0074

    authors: Texier P,Coddeville M,Bordes P,Genevaux P

    更新日期:2018-09-01 00:00:00

  • Generation of Flp-intm-ready DG44 and Lec 3.2.8.1 CHO cell lines for quick and easy constitutive protein expression.

    abstract::The well-characterized cell line Chinese hamster ovary (CHO) has been used to produce numerous biopharmaceuticals and is an important tool for basic research. However, introducing foreign DNA into specially modified CHO cells such as DG44 and Lec 3.2.8.1 can sometimes be an arduous process. Here we show that the Flp-i...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0075

    authors: Soler DC,Young AE,Vahedi-Faridi A,McCormick TS

    更新日期:2018-07-01 00:00:00

  • A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings.

    abstract::One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsi...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0044

    authors: Vang S,Seitz K,Krysan PJ

    更新日期:2018-06-01 00:00:00

  • Protein analysis: key to the future.

    abstract::Protein analysis is crucial to elucidating the function of proteins and understanding the impact of their presence, absence and alteration. This is key to advancing knowledge about diseases, providing the opportunity for biomarker discovery and development of therapeutics. In this issue of Tech News, Nawsheen Boodhun ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/btn-2018-0055

    authors: Boodhun N

    更新日期:2018-05-01 00:00:00

  • Development of a cost effective and robust AlphaScreen® platform for application.

    abstract::The use of AlphaScreen® detection has allowed researchers to examine a wide variety of molecular interactions for use in high-throughput screening. However, the cost of Alpha reagents can often be prohibitory for extended screening campaigns or for young investigators with limited funding. To reduce assay costs, many ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-2001

    authors: Veloria JR,Devkota AK,Cho EJ,Dalby KN

    更新日期:2018-04-01 00:00:00

  • BUILDING THE FUTURE FROM 3D BLUEPRINTS.

    abstract::3D printed biomaterials are increasingly used in cell cultures and drug screens. Given the ease of creating artificial tissues, will this technique revolutionize biomedicine and organ implants in the future? ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/btn-2017-0113

    authors: Prabhune M

    更新日期:2018-03-01 00:00:00

  • GOING LONG AND DIRECT.

    abstract::Sarah Webb explores how RNA sequencing innovations help scientists tackle the complexity of the transcriptome. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/btn-2017-0105

    authors: Webb S

    更新日期:2018-02-01 00:00:00

  • PART I: FIGHTING CANCER WITH DEADLY BACTERIA.

    abstract::A 200-year-old observation might provide a new way to eliminate tumors by infecting cancer patients with bacteria. Kristie Nybo explores a new approach that could transform cancer treatment. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/000114624

    authors: Nybo K

    更新日期:2018-01-01 00:00:00

  • DIRECTING DRUG EVOLUTION.

    abstract::Directed evolution is poised to change small molecule discovery and provide greater access to "drug space." Sarah Webb looks into the evolving drug discovery landscape. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/000114616

    authors: Webb S Ph D

    更新日期:2017-12-01 00:00:00

  • A TB40/E-derived human cytomegalovirus genome with an intact US-gene region and a self-excisable BAC cassette for immunological research.

    abstract::For immunological research on the human cytomegalovirus (HCMV), a virus that combines the broad cell tropism of clinical isolates, efficient replication in cell culture, the complete set of MHC-I modulator genes, and suitability for genetic engineering is desired. Here, we aimed to generate a genetically complete deri...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114606

    authors: Sampaio KL,Weyell A,Subramanian N,Wu Z,Sinzger C

    更新日期:2017-11-01 00:00:00

  • Genotyping live fish larvae: Non-lethal and noninvasive DNA isolation from 3-5 day old hatchlings.

    abstract::Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individuall...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114598

    authors: Espinoza GJ,Poland JM,Bremer JRA

    更新日期:2017-10-01 00:00:00

  • Preparation of highly multiplexed small RNA sequencing libraries.

    abstract::MicroRNAs (miRNAs) are ~22-nucleotide-long small non-coding RNAs that regulate the expression of protein-coding genes by base pairing to partially complementary target sites, preferentially located in the 3´ untranslated region (UTR) of target mRNAs. The expression and function of miRNAs have been extensively studied ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114574

    authors: Persson H,Søkilde R,Pirona AC,Rovira C

    更新日期:2017-08-01 00:00:00

  • Transient dominant host-range selection using Chinese hamster ovary cells to generate marker-free recombinant viral vectors from vaccinia virus.

    abstract::Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selec...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114537

    authors: Liu L,Cooper T,Eldi P,Garcia-Valtanen P,Diener KR,Howley PM,Hayball JD

    更新日期:2017-04-01 00:00:00

  • Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.

    abstract::S-palmitoylation (S-acylation) is emerging as an important dynamic post-translational modification of cysteine residues within proteins. Current assays for protein S-palmitoylation involve either in vivo labeling or chemical cleavage of S-palmitoyl groups to reveal a free cysteine sulfhydryl that can be subsequently l...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114516

    authors: Hurst CH,Turnbull D,Plain F,Fuller W,Hemsley PA

    更新日期:2017-02-01 00:00:00

  • BIG LABS AND TINY INSTRUMENTS.

    abstract::At many research institutions, lab space is more valuable than gold. Developers are taking note by designing smaller instruments with enhanced capabilities. Nathan Blow looks inside today's tiny lab. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/000114490

    authors: Blow NS Ph D

    更新日期:2017-01-01 00:00:00

  • [Letter to the Editor] Many commercial hot-start polymerases demonstrate activity prior to thermal activation.

    abstract::Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: martin.kennedy@otago.ac.nz. ...

    journal_title:BioTechniques

    pub_type: 信件

    doi:10.2144/000114481

    authors: Stevens AJ,Appleby S,Kennedy MA

    更新日期:2016-12-01 00:00:00

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