解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Presepsin is a 13-kDa N-terminal glycoprotein of CD14. Previously, agitation-induced increases in presepsin levels have been reported; however, the mechanism remains poorly understood. In this study, we aimed to reveal the mechanism of presepsin increase. The agitated plasma or serum was separated using gel exclusion ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0136
更新日期:2021-01-29 00:00:00
abstract::New cloning vectors have been developed with features to enhance quick allelic exchange in gram-negative bacteria. The conditionally replicative R6K and transfer origins facilitate conjugation and chromosomal integration into a variety of bacterial species, whereas the sacB gene provides counterselection for allelic e...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0135
更新日期:2021-01-25 00:00:00
abstract::Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with 'difficult-to-express' proteins. Cell-free pr...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0155
更新日期:2021-01-20 00:00:00
abstract::[Formula: see text] Known to be a sturdy weapon in a scientist's arsenal, how has the gene editing tool CRISPR been applied in the fight against COVID-19? ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/btn-2020-0145
更新日期:2020-11-01 00:00:00
abstract::S-100B is used in melanoma follow-up. This serum biomarker is also present in adipocytes; therefore, subcutaneous adipocytes trapped in the needle before performing a venipuncture could contaminate the serum. The aim was to study the influence of adipocyte contamination on blood samples used for S-100B analysis, possi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0147
更新日期:2020-11-01 00:00:00
abstract::Direct contact-based coculture of human dermal fibroblasts and epidermal keratinocytes has been a long-standing and challenging issue owing to different serum and growth factor requirements of the two cell types. Existing protocols employ high serum concentrations (up to 10% fetal bovine serum), complex feeder systems...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0112
更新日期:2020-11-01 00:00:00
abstract::The increasing interest in manipulating neural circuits in developing brains has created a demand for reliable and accurate methods for delivering viruses to newborn mice. Here we describe a novel 3D-printed mouse neonatal stereotaxic adaptor for intracerebral viral injection that provides enhanced precision and relia...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0050
更新日期:2020-10-01 00:00:00
abstract::Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities. This work describes application of the Gyrolab solution affinity module and the new...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0080
更新日期:2020-09-01 00:00:00
abstract::Polyethyleneimine (PEI) is a flocculent that is widely used in the downstream purification of monoclonal antibodies. It is an in-process residual that is carried through the drug purification process and strongly inhibits residual DNA quantitation by real-time quantitative PCR assay. Very high sample dilutions (e.g., ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2020-0011
更新日期:2020-06-01 00:00:00
abstract::Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended to medium- and low-...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0124
更新日期:2020-06-01 00:00:00
abstract::Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations...
journal_title:BioTechniques
pub_type: 信件
doi:10.2144/btn-2020-0012
更新日期:2020-06-01 00:00:00
abstract::Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0148
更新日期:2020-06-01 00:00:00
abstract::We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates. The technique is ideally suited for the generation of longer donor DNA sequences for CRISPR/Cas9-medi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0104
更新日期:2020-06-01 00:00:00
abstract::Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0172
更新日期:2020-05-01 00:00:00
abstract::Millions of museum specimens are integral to biodiversity studies; however, DNA degradation may limit the ability to obtain DNA sequences. In this study, a degradation analysis model for Lepidoptera specimens was established. Based on this model, we revealed the characteristics of DNA fragment distribution caused by e...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0166
更新日期:2020-03-01 00:00:00
abstract::Xer-cise is a technique using antibiotic resistance cassettes flanked by dif sites allowing spontaneous and accurate excision from bacterial chromosomes with a high frequency through the action of the cellular recombinase XerCD. Here, we report a significant improvement of Xer-cise in Mycobacteria. Zeocin resistance c...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0119
更新日期:2020-02-01 00:00:00
abstract::The CRISPR/Cas9 system is an efficient gene-editing method, but it is difficult to obtain mutants for some specific species and special genome structures. A previously reported multiplexed, semi-nested PCR target-enrichment approach, which does not rely on transgenic technology, has been shown to be an effective and a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0001
更新日期:2019-12-01 00:00:00
abstract::Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0071
更新日期:2019-10-01 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are subject to post-translational modifications. This allows the same polypeptide to be involved in different interaction networks with different consequences, ranging from regulatory signalling networks to the formation of membrane-less organelles. We report a robust method fo...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0033
更新日期:2019-07-01 00:00:00
abstract::We systematically evaluated the performance and reliability of several widely used, commercially available actin-filament probes in a highly motile breast adenocarcinoma cell line to optimize the visualization of F-actin-rich dynamic lamellipodia. We evaluated four Phalloidin-fluorophores, two anti-actin antibodies, a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0112
更新日期:2019-03-01 00:00:00
abstract::Naturstoff reagent A (diphenylboric acid 2-aminoethyl ester [DPBA]) has been used historically in plant science to observe polyphenolic pigments, such as flavonoids, whose fluorescence requires enhancement to be visible by microscopy. Flavonoids are common dietary constituents and are the focus of considerable attenti...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0084
更新日期:2019-02-01 00:00:00
abstract::PhDs, postdocs, fellows and newly appointed lecturers all fall into the category of early-career researchers (ECRs). The life of researchers in early stages of their career is far from straightforward. Funding is a huge barrier for many ECRs looking to make the next step in their career, but it's not the only challeng...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/btn-2018-0182
更新日期:2019-01-01 00:00:00
abstract::Sample automation and management is increasingly important as the number and size of population-scale and high-throughput projects grow. This is particularly the case in large-scale population studies where sample size is far outpacing the commonly used 96-well plate format. To facilitate management and transfer of sa...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0090
更新日期:2018-12-01 00:00:00
abstract::Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax® as a tool for st...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0062
更新日期:2018-11-01 00:00:00
abstract::A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this wor...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0074
更新日期:2018-09-01 00:00:00
abstract::The well-characterized cell line Chinese hamster ovary (CHO) has been used to produce numerous biopharmaceuticals and is an important tool for basic research. However, introducing foreign DNA into specially modified CHO cells such as DG44 and Lec 3.2.8.1 can sometimes be an arduous process. Here we show that the Flp-i...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0075
更新日期:2018-07-01 00:00:00
abstract::One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0044
更新日期:2018-06-01 00:00:00
abstract::Protein analysis is crucial to elucidating the function of proteins and understanding the impact of their presence, absence and alteration. This is key to advancing knowledge about diseases, providing the opportunity for biomarker discovery and development of therapeutics. In this issue of Tech News, Nawsheen Boodhun ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/btn-2018-0055
更新日期:2018-05-01 00:00:00
abstract::The use of AlphaScreen® detection has allowed researchers to examine a wide variety of molecular interactions for use in high-throughput screening. However, the cost of Alpha reagents can often be prohibitory for extended screening campaigns or for young investigators with limited funding. To reduce assay costs, many ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-2001
更新日期:2018-04-01 00:00:00
abstract::3D printed biomaterials are increasingly used in cell cultures and drug screens. Given the ease of creating artificial tissues, will this technique revolutionize biomedicine and organ implants in the future? ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/btn-2017-0113
更新日期:2018-03-01 00:00:00
abstract::Sarah Webb explores how RNA sequencing innovations help scientists tackle the complexity of the transcriptome. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/btn-2017-0105
更新日期:2018-02-01 00:00:00
abstract::A 200-year-old observation might provide a new way to eliminate tumors by infecting cancer patients with bacteria. Kristie Nybo explores a new approach that could transform cancer treatment. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114624
更新日期:2018-01-01 00:00:00
abstract::Directed evolution is poised to change small molecule discovery and provide greater access to "drug space." Sarah Webb looks into the evolving drug discovery landscape. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114616
更新日期:2017-12-01 00:00:00
abstract::For immunological research on the human cytomegalovirus (HCMV), a virus that combines the broad cell tropism of clinical isolates, efficient replication in cell culture, the complete set of MHC-I modulator genes, and suitability for genetic engineering is desired. Here, we aimed to generate a genetically complete deri...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114606
更新日期:2017-11-01 00:00:00
abstract::Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individuall...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114598
更新日期:2017-10-01 00:00:00
abstract::MicroRNAs (miRNAs) are ~22-nucleotide-long small non-coding RNAs that regulate the expression of protein-coding genes by base pairing to partially complementary target sites, preferentially located in the 3´ untranslated region (UTR) of target mRNAs. The expression and function of miRNAs have been extensively studied ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114574
更新日期:2017-08-01 00:00:00
abstract::Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selec...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114537
更新日期:2017-04-01 00:00:00
abstract::S-palmitoylation (S-acylation) is emerging as an important dynamic post-translational modification of cysteine residues within proteins. Current assays for protein S-palmitoylation involve either in vivo labeling or chemical cleavage of S-palmitoyl groups to reveal a free cysteine sulfhydryl that can be subsequently l...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114516
更新日期:2017-02-01 00:00:00
abstract::At many research institutions, lab space is more valuable than gold. Developers are taking note by designing smaller instruments with enhanced capabilities. Nathan Blow looks inside today's tiny lab. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114490
更新日期:2017-01-01 00:00:00
abstract::Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: martin.kennedy@otago.ac.nz. ...
journal_title:BioTechniques
pub_type: 信件
doi:10.2144/000114481
更新日期:2016-12-01 00:00:00