Abstract:
:Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our method is fast, easy to execute, and economically advantageous compared to expensive commercial kits, making it useful for high-throughput experiments, incorporation of nonstandard amino acids, and cell-free metabolic engineering.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Caschera F,Noireaux Vdoi
10.2144/000114249subject
Has Abstractpub_date
2015-01-01 00:00:00pages
40-3issue
1eissn
0736-6205issn
1940-9818pii
000114249journal_volume
58pub_type
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