Abstract:
:We have developed an automated purification method for dye-terminator-based DNA sequencing products using a magnetic bead approach. This 384-well protocol generates sequence fragments that are essentially free of template DNA, salt, and excess dye-terminator products. In comparison with traditional ethanol precipitation protocols, this method uses no centrifugation, is rapid, completely automated, and increases the phred-20 read length by an average of 40 bases. To date, we have processed over 4 million samples with 94% averaging 641 phred-20 bases on the MegaBACE 1000 and 4000 and the ABI PRISM 3700 capillary instruments.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Elkin C,Kapur H,Smith T,Humphries D,Pollard M,Hammon N,Hawkins Tdoi
10.2144/02326st05subject
Has Abstractpub_date
2002-06-01 00:00:00pages
1296, 1298-1300, 1302issue
6eissn
0736-6205issn
1940-9818journal_volume
32pub_type
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