Abstract：
:A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from polysaccharide- and proteoglycan-rich material. In the modified procedure, RNA is precipitated from the aqueous phase by the combined action of isopropanol and a high-salt concentration. Under these conditions, RNA is effectively precipitated while contaminating polysaccharides and proteoglycans remain in the soluble form. The modified precipitation does not prolong or increase the complexity of the TRI Reagent procedure. The new procedure was tested by isolation of RNA from polysaccharide- and proteoglycan-rich tissues such as rat liver and aorta.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Chomczynski P,Mackey Ksubject
Has Abstractpub_date
1995-12-01 00:00:00pages
942-5issue
6eissn
0736-6205issn
1940-9818journal_volume
19pub_type
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