Abstract：
:Recombinant retroviral vectors usually encode the genes of interest in place of the viral structural genes, which must be provided in trans. These viruses are therefore defective for replication: infected cells cannot produce progeny virus. However, in some cases it may be desirable to generate virus from an infected cell clone displaying a phenotype of interest. We describe a rapid method for producing virus, which involves fusing the infected cells to fresh packaging cells. Stable producer lines are generated after fusion by co-selecting for the Ecogpt+ marker in the packaging cells and the G418 resistance (neor) marker in the infected cells. We have used this method to develop cell lines that produce retroviruses encoding a Leu 61-activated c-Ha-ras oncogene as well as a neor gene. The viruses confer oncogenic transformation on 95%-100% of infected target cells as assayed by altered morphology, focus formation and soft agar growth.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Corbley MJ,Cherington V,Feig LA,Cooper GM,Roberts TMsubject
Has Abstractpub_date
1994-12-01 00:00:00pages
1102-3, 1106-9issue
6eissn
0736-6205issn
1940-9818journal_volume
17pub_type
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