Abstract：
:Genetic immunization is a simple method for producing polyclonal antibodies in mice. To test if this approach could be used for monoclonal antibody production, biolistic transfection was used to immunize a mouse. High levels of polyclonal antibodies against human growth hormone (hGH) were elicited following three inoculations with the gene for hGH. When hybridoma cells were created from the mouse's splenocytes, approximately 17% secreted antibodies vs. hGH. Of these, some recognized only native or denatured hGH, while most recognized both forms of the protein. These findings demonstrate the utility of genetic immunization as a method to produce monoclonal antibodies.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Barry MA,Barry ME,Johnston SAsubject
Has Abstractpub_date
1994-04-01 00:00:00pages
616-8, 620issue
4eissn
0736-6205issn
1940-9818journal_volume
16pub_type
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