Abstract：
:To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in the initial mRNA differential display. These included a restriction site to aid cloning. PCR began with one low-stringency cycle (40 degrees C for annealing) followed by 35 high-stringency cycles (60 degrees C for annealing). The modified method significantly improved the reproducibility and sensitivity of the mRNA differential display while still keeping the characteristics of the original method.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Zhao S,Ooi SL,Pardee ABsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
842-6, 848, 850issue
5eissn
0736-6205issn
1940-9818journal_volume
18pub_type
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