Abstract:
:Recombinant alphaviruses have been used as vehicles for delivery and expression of heterologous genes in mammalian, avian and insect cell lines. We have used a Sindbis replicon virus (Sinreplac) able to express the E. coli lacZ gene to compare the efficiency of transduction in one insect, six mammalian cell lines and cultured rat dorsal neurons which apparently express beta-galactosidase over a 30-day time period. Results show that different cell lines were transduced with varying degrees of efficiency and that this efficiency could be improved in some cell lines by packaging the replicon with a helper derived from a more neurovirulent strain of Sindbis.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Corsini J,Traul DL,Wilcox CL,Gaines P,Carlson JOdoi
10.2144/96213rr02subject
Has Abstractpub_date
1996-09-01 00:00:00pages
492-7issue
3eissn
0736-6205issn
1940-9818journal_volume
21pub_type
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