Abstract:
:We have developed a novel vector pTCS, as a tool for efficient selection of open reading frame (ORF)-containing inserts. In pTCS clones containing an insert with an ORF a downstream marker gene (immE3, conferring resistance to colicin) is activated via translational coupling with the insert, and transformed cells can then be selected by exposure to colicin E3. Our method differs from previous methods in that the marker gene is activated without protein fusion, and that selection occurs irrespective of the reading frame of the insert.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ohashi-Kunihiro S,Yohda M,Masaki H,Machida Mdoi
10.2144/000112629subject
Has Abstractpub_date
2007-12-01 00:00:00pages
751-2, 754issue
6eissn
0736-6205issn
1940-9818pii
000112629journal_volume
43pub_type
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