Software to improve transfer and reproducibility of cell culture methods.

Abstract:

:Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax® as a tool for standardizing and transferring a complex cell culture protocol. The software workflow and template were used to electronically format a cardiomyocyte differentiation protocol and share a digitally executable copy with a different lab user. While the protocol was unfamiliar to the recipient, they executed the experiment by solely using CultureTrax and successfully derived cardiomyocytes from human induced pluripotent stem cells. This software tool significantly reduced the time and resources required to effectively transfer and implement a novel protocol.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Canfield SG,Jin G,Palecek SP,Sampsell T

doi

10.2144/btn-2018-0062

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

289-292

issue

5

eissn

0736-6205

issn

1940-9818

journal_volume

65

pub_type

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