Abstract:
:We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made between the Luminex OLA and both pyrosequencing and direct sequencing. Experiments to assess conversion rates, call rates, and concordance across technical replicates are also presented.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Bruse S,Moreau M,Azaro M,Zimmerman R,Brzustowicz Ldoi
10.2144/000112960subject
Has Abstractpub_date
2008-11-01 00:00:00pages
559-71issue
5eissn
0736-6205issn
1940-9818pii
000112960journal_volume
45pub_type
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