Abstract:
:A novel method for large-scale plasmid preparation is described. Crude extracts are subjected to acidic phenol extraction to remove any contaminants present in the aqueous phase. The supercoiled plasmid DNA, which preferentially remains in the organic phase and inter-phase, is extracted back into the aqueous phase with 1.5 M TRIZMA base, from which it is precipitated. The resultant plasmid DNA is highly pure and satisfactory for any subsequent procedures. The method is extremely economical and takes only 3-4 h.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Wang Z,Rossman TGsubject
Has Abstractpub_date
1994-03-01 00:00:00pages
460-3issue
3eissn
0736-6205issn
1940-9818journal_volume
16pub_type
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