Abstract:
:A method was developed for the detection of bacterial mRNAs using reverse transcriptase followed by the polymerase chain reaction (PCR) and Southern blot analysis. The method involves brief inhibition of protein synthesis with chloramphenicol, followed by reverse transcription, PCR amplification of cDNA and Southern blot hybridization. Detection of mRNAs by reverse transcription-PCR-Southern blot analysis is orders of magnitude more sensitive than Northern blot hybridization.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Mahbubani MH,Bej AK,Miller RD,Atlas RM,DiCesare JL,Haff LAsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
48-9issue
1eissn
0736-6205issn
1940-9818journal_volume
10pub_type
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