Abstract:
:Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification of transgenic founders. Given its speed and low cost, we anticipate that the adoption of this method will streamline DNA isolation for zebrafish research.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Meeker ND,Hutchinson SA,Ho L,Trede NSdoi
10.2144/000112619subject
Has Abstractpub_date
2007-11-01 00:00:00pages
610, 612, 614issue
5eissn
0736-6205issn
1940-9818pii
000112619journal_volume
43pub_type
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