Abstract:
:A head-to-tail trimer of the SV40 Bcl I-Bam H1 DNA fragment, specifying polyadenylation of RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of the trimer into several expression vectors efficiently prevented spurious expression of reporter genes resulting from transcriptional initiation in prokaryotic plasmid sequences in transfected mammalian cells.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Maxwell IH,Harrison GS,Wood WM,Maxwell Fsubject
Has Abstractpub_date
1989-03-01 00:00:00pages
276-80issue
3eissn
0736-6205issn
1940-9818journal_volume
7pub_type
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