Abstract:
:DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshifts in whole extracts. This method correlates purified factor seen indirectly by bandshift with factor that is purified and released, because they are the same.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Ren L,Chen H,Sternberg EAsubject
Has Abstractpub_date
1994-05-01 00:00:00pages
852-5issue
5eissn
0736-6205issn
1940-9818journal_volume
16pub_type
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