Samasy: an automated system for sample selection and robotic transfer.

Abstract:

:Sample automation and management is increasingly important as the number and size of population-scale and high-throughput projects grow. This is particularly the case in large-scale population studies where sample size is far outpacing the commonly used 96-well plate format. To facilitate management and transfer of samples in this format, we present Samasy, a web-based application for the construction of a sample database, intuitive display of sample and batch information, and facilitation of automated sample transfer or subset. Samasy is designed with ease-of-use in mind, can be quickly set up, and runs in any web browser.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Cario CL,Witte JS

doi

10.2144/btn-2018-0090

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

357-360

issue

6

eissn

0736-6205

issn

1940-9818

journal_volume

65

pub_type

杂志文章
  • Genetically encoded far-red fluorescent sensors for caspase-3 activity.

    abstract::Caspase-3 is a key effector caspase that is activated in both extrinsic and intrinsic pathways of apoptosis. Available fluorescent sensors for caspase-3 activity operate in relatively short wavelength regions and are nonoptimal for multiparameter microscopy and whole-body imaging. In the present work, we developed new...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114377

    authors: Zlobovskaya OA,Sergeeva TF,Shirmanova MV,Dudenkova VV,Sharonov GV,Zagaynova EV,Lukyanov KA

    更新日期:2016-02-01 00:00:00

  • Antibiotic-free bacterial strain selection using antisense peptide nucleic acid.

    abstract::Antibiotics are widely useful in medicine, agriculture, and industrial fermentations. However, increasing problems with resistant strains call for restrained use and alternative strategies. Antisense peptide nucleic acids (PNAs) show potent bactericidal effects when targeted against the essential Escherichia coli acpP...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Dryselius R,Nekhotiaeva N,Nielsen PE,Good L

    更新日期:2003-11-01 00:00:00

  • Detection and molecular mass determination of an HIV replication-enhancing female genital tract factor using a blot bioassay.

    abstract::We previously reported that cervicovaginal lavages (CVL) contain a factor that enhances the replication of human immunodeficiency virus (HIV) by increasing virus transcription in T cells and monocytoid cells. This factor was named the HIV-inducing factor (HIF). To determine the molecular mass of HIF, we adapted a blot...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/00283st05

    authors: Hashemi FB,Spear GT,Madsen L,Mollenhauer J

    更新日期:2000-03-01 00:00:00

  • Development and application of a nonradioactive phosphorescent autoradiograph marker.

    abstract::A simple and rapid method for permanently marking autoradiographs is described. This procedure is based on the phosphorescence of light-activated zinc sulfide and the subsequent exposure of x-ray films by this light emission. A lacquer-based carrier allows the zinc sulfide to remain in suspension and permits permanent...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Seto D,Rohrabacher C

    更新日期:1991-05-01 00:00:00

  • A novel yeast-based recombination method to clone and propagate diverse HIV-1 isolates.

    abstract::Replication studies on human immunodeficiency virus 1 (HIV-1) rely on a few laboratory strains that are divergent from dominant HIV-1 subtypes in the epidemic. Several phenotypic differences between diverse HIV-1 isolates and subtypes could affect vaccine development and treatment, but this research field lacks robust...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000113119

    authors: Dudley DM,Gao Y,Nelson KN,Henry KR,Nankya I,Gibson RM,Arts EJ

    更新日期:2009-05-01 00:00:00

  • Quantitation of the ras gene product in leukemic blast cells using enzymatic staining.

    abstract::Few studies have focused on the significance of ras protein levels in human malignancy, in part because of the inherent difficulty in quantitation of the ras gene product. We have developed a method for the enzymatic determination of the ras gene product and have used this method for the quantitation of ras gene produ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Gutheil JC,Mane SM,Bass KA,Lee EJ,Needleman SW

    更新日期:1990-08-01 00:00:00

  • Detection of Glu-Glu-tagged proteins in mammalian cell culture media by dot immunoblotting.

    abstract::A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatment were used to optimize the signal-to-noise ratio of the defined pro...

    journal_title:BioTechniques

    pub_type:

    doi:10.2144/00285st07

    authors: Ellsworth JL,Hamacher N,Bagwell N,West JW

    更新日期:2000-05-01 00:00:00

  • Sea urchin embryo as a model organism for the rapid functional screening of tubulin modulators.

    abstract::Identification of antimitotic molecules that affect tubulin dynamics is a multistep procedure. It includes in vitro tubulin polymerization assay, studies of a cell cycle effect, and general cytotoxicity assessment. To simplify this lengthy screening protocol, we have introduced and validated an assay system based on t...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000112193

    authors: Semenova MN,Kiselyov A,Semenov VV

    更新日期:2006-06-01 00:00:00

  • PBXL-1: a new fluorochrome applied to detection of proteins on membranes.

    abstract::An easy, sensitive and direct fluorescent immunodetection method for proteins is described using the new fluorochrome PBXL-1 imaged with the FMBIO II Laser Scanning Imaging System. PBXL-1 is derived from a protein supra-molecular complex that contains a large number of chromophores. This complex, the phycobilisome, is...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/99263pf02

    authors: Morseman JP,Moss MW,Zoha SJ,Allnutt FC

    更新日期:1999-03-01 00:00:00

  • Rapid regeneration of virus from cells infected with a retroviral vector.

    abstract::Recombinant retroviral vectors usually encode the genes of interest in place of the viral structural genes, which must be provided in trans. These viruses are therefore defective for replication: infected cells cannot produce progeny virus. However, in some cases it may be desirable to generate virus from an infected ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Corbley MJ,Cherington V,Feig LA,Cooper GM,Roberts TM

    更新日期:1994-12-01 00:00:00

  • Use of short-lived green fluorescent protein for the detection of proteasome inhibition.

    abstract::Human embryonic kidney (HEK293) cells were stably transduced with a retroviral vector containing an expression cassette for a short-lived green fluorescent protein (d2EGFP) and the neomycin resistance gene (Neor). When Neor HEK293 clones were treated with proteasome inhibitors, lactacystin or MG132, an increase in the...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01303dd03

    authors: Andreatta C,Nahreini P,Hovland AR,Kumar B,Edwards-Prasad J,Prasad KN

    更新日期:2001-03-01 00:00:00

  • Green fluorescent protein as a reporter of gene expression and protein localization.

    abstract::The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is rapidly becoming an important reporter molecule for monitoring gene expression and protein localization in vivo, in situ and in real time. GFP emits bright green light (lambda max = 509 nm) when excited with UV or blue light (lambda max = 395 ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Kain SR,Adams M,Kondepudi A,Yang TT,Ward WW,Kitts P

    更新日期:1995-10-01 00:00:00

  • Absolute quantitation of cancer-related proteins using an MS-based peptide chip.

    abstract::New technologies are needed that can diagnose cancer more rapidly and accurately. These technologies must also have the ability to identify the particular cellular abnormalities contributing to the malignancy, thus directing the appropriate treatments. Such technologies should permit absolute quantitation of specific ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/05386su01

    authors: Warren EN,Jiang J,Parker CE,Borchers CH

    更新日期:2005-06-01 00:00:00

  • Protein aggregation mediated by cysteine oxidation during the stacking phase of discontinuous buffer SDS-PAGE.

    abstract::The resolution of complex protein mixtures by discontinuous buffer SDS-PAGE is accomplished by their concentration into thin bands in the stacking gel, followed by their separation during migration through the resolving gel. Recombinant human interferon-inducible protein-10 (IP-10), a 10-kDa C-X-C chemokine with four ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01302st04

    authors: Crow MK,Karasavvas N,Sarris AH

    更新日期:2001-02-01 00:00:00

  • Destabilized green fluorescent protein detects rapid removal of transcription blocks after genotoxic exposure.

    abstract::High stabilities of reporter proteins and their messenger RNAs (mRNAs) interfere with the detection of rapid transient changes in gene expression, such as transcriptional blocks posed by genotoxic DNA lesions. We have modified a green fluorescent protein (GFP) gene within the episomal pMARS vector by addition of a fra...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000112479

    authors: Kitsera N,Khobta A,Epe B

    更新日期:2007-08-01 00:00:00

  • Real-time measurement of PMA-induced cellular alterations by microelectrode array-based impedance spectroscopy.

    abstract::For a feasible and cost-effective impedance measurement of cellular alterations in real-time, we combined commercially available microelectrode arrays (MEAs), consisting of 60 microelectrodes, with a conventional impedance analyzer. For proof of principle, a breast carcinoma cell line (MCF-7) was cultured on MEAs, and...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000112254

    authors: Rothermel A,Nieber M,Müller J,Wolf P,Schmidt M,Robitzki AA

    更新日期:2006-10-01 00:00:00

  • Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

    abstract::Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small blo...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Walsh PS,Metzger DA,Higuchi R

    更新日期:1991-04-01 00:00:00

  • Use of an ALFexpress DNA sequencer to analyze protein-nucleic acid interactions by band shift assay.

    abstract::Gel retardation analysis, or band shift assay, is technically the simplest method to investigate protein-nucleic acid interactions. In this report, we describe a nonradioactive band shift assay using a fluorescent DNA target and an ALFexpress automatic DNA sequencer in place of the current method that utilizes radioac...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01305rr03

    authors: Filée P,Delmarcelle M,Thamm I,Joris B

    更新日期:2001-05-01 00:00:00

  • Transient dominant host-range selection using Chinese hamster ovary cells to generate marker-free recombinant viral vectors from vaccinia virus.

    abstract::Recombinant vaccinia viruses (rVACVs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. Two common methods for selection during construction of rVACV clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selec...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114537

    authors: Liu L,Cooper T,Eldi P,Garcia-Valtanen P,Diener KR,Howley PM,Hayball JD

    更新日期:2017-04-01 00:00:00

  • Measurement of isolated myocyte volume using the Coulter models Z2 and ZM/C256: a comparison of instrument function.

    abstract::Changes in cardiac structure that depart from normal have generally been termed "remodeling". Assessment of ventricular remodeling at the cellular level should include measurement of myocyte dimensions. A well-established and reliable method to assess myocyte remodeling uses isolated cells and the Coulter Counter/Chan...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/98253pf02

    authors: Said S,Tamura T,Gerdes AM

    更新日期:1998-09-01 00:00:00

  • Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.

    abstract::S-palmitoylation (S-acylation) is emerging as an important dynamic post-translational modification of cysteine residues within proteins. Current assays for protein S-palmitoylation involve either in vivo labeling or chemical cleavage of S-palmitoyl groups to reveal a free cysteine sulfhydryl that can be subsequently l...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114516

    authors: Hurst CH,Turnbull D,Plain F,Fuller W,Hemsley PA

    更新日期:2017-02-01 00:00:00

  • A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis.

    abstract::A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Fernández-Silva P,Enriquez JA,Montoya J

    更新日期:1992-04-01 00:00:00

  • An improved Huffman coding method for archiving text, images, and music characters in DNA.

    abstract::An improved Huffman coding method for information storage in DNA is described. The method entails the utilization of modified unambiguous base assignment that enables efficient coding of characters. A plasmid-based library with efficient and reliable information retrieval and assembly with uniquely designed primers is...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000113218

    authors: Ailenberg M,Rotstein O

    更新日期:2009-09-01 00:00:00

  • Eliciting antigen-specific egg-yolk IgY with naked DNA.

    abstract::Immunization with naked DNA was used to elicit chicken egg yolk antibodies (IgY). Layer hens were inoculated with plasmid DNA encoding the enhanced green fluorescent protein, the fusion protein of Newcastle disease virus, and VP2 of African horse sickness virus. IgY was extracted from egg yolks by polyethylene glycol ...

    journal_title:BioTechniques

    pub_type:

    doi:10.2144/01313dd05

    authors: Romito M,Viljoen GJ,Du Plessis DH

    更新日期:2001-09-01 00:00:00

  • Hematopoietic cells from mice that are deficient in both Bcrp1/Abcg2 and Mdr1a/1b develop normally but are sensitized to mitoxantrone.

    abstract::Hematopoietic stem cells (HSCs) express Mdr1a/1b and Bcrp1/Abcg2, which are members of the ATP binding cassette transporter family. Mice lacking both Mdr1-type genes (Mdr1a and Mdr1b) or Bcrp1 had normal hematopoietic development, but it has been unclear whether Mdr1a/1b and Bcrp1 play redundant roles in hematopoiesis...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/03356ss04

    authors: Zhou S,Zong Y,Lu T,Sorrentino BP

    更新日期:2003-12-01 00:00:00

  • Generation and characterization of recombinant vascular targeting agents from hybridoma cell lines.

    abstract::Vascular targeting agents (VTAs) can be produced by linking antibodies or antibody fragments directed against endothelial cell markers to effector moieties. So far, it has been necessary to produce the components of VTAs (antibody, antibody fragment, linker, and effector) separately and, subsequently, to conjugate the...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01301dd03

    authors: Gottstein C,Wels W,Ober B,Thorpe PE

    更新日期:2001-01-01 00:00:00

  • Single cell analysis of transfected gene expression in primary heart cultures containing multiple cell types.

    abstract::Transfection experiments involving mixed cell cultures pose special problems because of differential uptake and expression of DNA by different cell types. In order to evaluate the expression of transfected DNA in primary cultures of embryonic myocardial cells, we have used immunofluorescence microscopy to analyze expr...

    journal_title:BioTechniques

    pub_type: 杂志文章,评审

    doi:

    authors: Antin PB,Mar JH,Ordahl CP

    更新日期:1988-07-01 00:00:00

  • Generation of high-titer retroviral vectors following receptor-mediated, adenovirus-augmented transfection.

    abstract::A new procedure is described for the generation of high-titer, helper-free retrovirus vectors employing receptor-mediated, adenovirus-augmented transfection into a standard packaging cell line. Viral titers are increased 30-fold to 100-fold in transiently (> 10(5) infectious units per mL) and stable (> 10(7) infectiou...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: von Rüden T,Stingl L,Cotten M,Wagner E,Zatloukal K

    更新日期:1995-03-01 00:00:00

  • Efficacy of an amphipathic oligopeptide to shuttle and release a cis-acting DNA decoy into human cells.

    abstract::We investigated the ability of an amphipathic oligopeptide to carry a synthetic dsDNA oligonucleotide inside human cells. The oligonucleotide was designed as a decoy binding site for the transcriptional activator of the methylguanine-DNA methyltransferase (MGMT) gene. The complex oligopeptide and decoy were administer...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/02321dd03

    authors: Citti L,Rovero P,Colombo MG,Mariani L,Poliseno L,Rainaldi G

    更新日期:2002-01-01 00:00:00

  • A simple method for genetic typing of transferrins in nonhuman primates.

    abstract::The serum protein transferrin (Tf) is a valuable marker for genetic studies of primates, because it is usually polymorphic, exhibiting as many as 13 allelic forms with high heterozygosity. The standard procedure to detect the different phenotypes requires vertical electrophoresis on polyacrylamide gels for 18 h at 4 d...

    journal_title:BioTechniques

    pub_type:

    doi:

    authors: Manis GS,Samples NK,Stone WH

    更新日期:1993-11-01 00:00:00