Abstract：
:The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight. In the absence of Cre, the red fluorescent protein is expressed; when Cre catalyzes a recombination event, the green fluorescent protein is produced. Testing this system in transiently transfected cells showed that it produced robust signals (90% of the cells converted from red to green) when equal amounts of the plasmids encoding Cre recombinase and the Cre Stoplight were used. A 1:100 ratio of enzyme to reporter plasmid produced similar results, and a 1:10000 ratio was necessary to significantly reduce the number of cells converting to green (1%).
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Yang YS,Hughes TEdoi
10.2144/01315st03subject
Has Abstractpub_date
2001-11-01 00:00:00pages
1036, 1038, 1040-1issue
5eissn
0736-6205issn
1940-9818journal_volume
31pub_type
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