Abstract:
:High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Yoon JR,Laible PD,Gu M,Scott HN,Collart FRdoi
10.2144/02336bc03subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1328-33issue
6eissn
0736-6205issn
1940-9818journal_volume
33pub_type
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