Abstract:
:Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Johansson P,Muslimovic A,Hultborn R,Fernström E,Hammarsten Odoi
10.2144/000113738subject
Has Abstractpub_date
2011-09-01 00:00:00pages
185-9issue
3eissn
0736-6205issn
1940-9818pii
000113738journal_volume
51pub_type
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