Abstract:
:When PCR is carried out in a polyacrylamide gel, each target molecule forms a molecular colony that comprises many copies of the original template. By counting the number of colonies, one can directly determine the target titer, with 100% of the DNA molecules and approximately 15% of the RNA molecules being detected. Furthermore, because of the spatial separation of the products in the gel, no interference is observedfrom another simultaneously amplified target even if it is present at a 106 higher amount orfrom human nucleic acids that outweigh the target by up to a factor of 1,012, which is often true of clinical samples. All these features provide for an accurate and reliable assay of viruses even at very low amounts, that is, in cases most important to diagnostics.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Chetverina HV,Samatov TR,Ugarov VI,Chetverin ABdoi
10.2144/02331md03subject
Has Abstractpub_date
2002-07-01 00:00:00pages
150-2, 154, 156issue
1eissn
0736-6205issn
1940-9818journal_volume
33pub_type
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